http://2013.igem.org/wiki/index.php?title=Team:EPF_Lausanne/Calendar/5_September_2013&feed=atom&action=historyTeam:EPF Lausanne/Calendar/5 September 2013 - Revision history2024-03-29T10:45:49ZRevision history for this page on the wikiMediaWiki 1.16.5http://2013.igem.org/wiki/index.php?title=Team:EPF_Lausanne/Calendar/5_September_2013&diff=289447&oldid=prevSandelisa90 at 10:19, 4 October 20132013-10-04T10:19:54Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Primer designing for a new strategy to create the following fusion proteins: INP_YFP_strepta and INP_strepta_YFP.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Primer designing for a new strategy to create the following fusion proteins: INP_YFP_strepta and INP_strepta_YFP.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><font size = "4"> Sensing-Effector </font> <BR></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><font size = "4"> Sensing-Effector </font> <BR></div></td></tr>
</table>Sandelisa90http://2013.igem.org/wiki/index.php?title=Team:EPF_Lausanne/Calendar/5_September_2013&diff=289444&oldid=prevSandelisa90 at 10:19, 4 October 20132013-10-04T10:19:43Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Sending Gibson Alive Miniprep product for sequencing using VR and VF2 iGEM primers.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Sending Gibson Alive Miniprep product for sequencing using VR and VF2 iGEM primers.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>- Sending Gibson Dead and iGEM miniprep for sequencing using VF2 primer once we didn't do it last time. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>- Sending Gibson Dead and iGEM miniprep for sequencing using VF2 primer once we didn't do it last time. </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>''New Strategy''<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>''New Strategy''<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Primer designing for a new strategy to create the following fusion proteins: INP_YFP_strepta and INP_strepta_YFP.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Primer designing for a new strategy to create the following fusion proteins: INP_YFP_strepta and INP_strepta_YFP.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><br><br></ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><font size = "4"> Sensing-Effector </font> <BR></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><font size = "4"> Sensing-Effector </font> <BR></div></td></tr>
</table>Sandelisa90http://2013.igem.org/wiki/index.php?title=Team:EPF_Lausanne/Calendar/5_September_2013&diff=289441&oldid=prevSandelisa90 at 10:19, 4 October 20132013-10-04T10:19:20Z<p></p>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><font size = "4"> Cell Surface Display</font> <BR></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">''Sequencing''<br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">- Sending Gibson Alive Miniprep product for sequencing using VR and VF2 iGEM primers.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><br>- Sending Gibson Dead and iGEM miniprep for sequencing using VF2 primer once we didn't do it last time. </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><br>''New Strategy''<br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Primer designing for a new strategy to create the following fusion proteins: INP_YFP_strepta and INP_strepta_YFP.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><br><br></ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><font size = "4"> Sensing-Effector </font> <BR></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><font size = "4"> Sensing-Effector </font> <BR></div></td></tr>
</table>Sandelisa90http://2013.igem.org/wiki/index.php?title=Team:EPF_Lausanne/Calendar/5_September_2013&diff=281448&oldid=prevGigiGoesGugu at 19:09, 3 October 20132013-10-03T19:09:39Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><font size = "4"> Sensing </font> <BR></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><font size = "4"> Sensing<ins class="diffchange diffchange-inline">-Effector </ins></font> <BR></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''PCR of the two pH sensitive Promoters and the constitutive promoter from iGEM'' <BR></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''PCR of the two pH sensitive Promoters and the constitutive promoter from iGEM'' <BR></div></td></tr>
</table>GigiGoesGuguhttp://2013.igem.org/wiki/index.php?title=Team:EPF_Lausanne/Calendar/5_September_2013&diff=278823&oldid=prevGigiGoesGugu at 16:37, 3 October 20132013-10-03T16:37:16Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Purification of PCR products'' <BR></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Purification of PCR products'' <BR></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>After PCR we purified the products with the qiagen Kit according to the manufacturers instructions. As the pH promoter from iGEM is only 30 bp it was removed during purification. So we decided to amplify it again but with primers that had overhangs with the backbone, in order to have an insert of about 100 bp. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">-</ins>After PCR we purified the products with the qiagen Kit according to the manufacturers instructions. As the pH promoter from iGEM is only 30 bp it was removed during purification. So we decided to amplify it again but with primers that had overhangs with the backbone, in order to have an insert of about 100 bp. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The final concentrations of the purifications were all very low. I still did a DpnI digestion with 8 ul DNA and then a Gibson assembly but it did not work. As I had done only a 20 ul reaction of the PCR, I had no more Product left so I decided to do the PCR again but with a volume of 50 ul.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The final concentrations of the purifications were all very low. I still did a DpnI digestion with 8 ul DNA and then a Gibson assembly but it did not work. As I had done only a 20 ul reaction of the PCR, I had no more Product left so I decided to do the PCR again but with a volume of 50 ul.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><BR><BR></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><BR><BR></div></td></tr>
</table>GigiGoesGuguhttp://2013.igem.org/wiki/index.php?title=Team:EPF_Lausanne/Calendar/5_September_2013&diff=278810&oldid=prevGigiGoesGugu at 16:36, 3 October 20132013-10-03T16:36:31Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Sensing <BR></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><font size = "4"> </ins>Sensing <ins class="diffchange diffchange-inline"></font> </ins><BR></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">'</del>''PCR of the two pH sensitive Promoters and the constitutive promoter from iGEM<del class="diffchange diffchange-inline">'</del>'' <BR></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>''PCR of the two pH sensitive Promoters and the constitutive promoter from iGEM'' <BR></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>We used the same program as the day before in order to amplify the promoters. For the hya and the Cad promoters we put 3 min initial denaturation as they were contained in genomic DNA. Again the PCR was successful</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">-</ins>We used the same program as the day before in order to amplify the promoters. For the hya and the Cad promoters we put 3 min initial denaturation as they were contained in genomic DNA. Again the PCR was successful</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>''Purification of PCR products'' <BR></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">'</del>''Purification of PCR products<del class="diffchange diffchange-inline">'</del>'' <BR></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>After PCR we purified the products with the qiagen Kit according to the manufacturers instructions. As the pH promoter from iGEM is only 30 bp it was removed during purification. So we decided to amplify it again but with primers that had overhangs with the backbone, in order to have an insert of about 100 bp. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>After PCR we purified the products with the qiagen Kit according to the manufacturers instructions. As the pH promoter from iGEM is only 30 bp it was removed during purification. So we decided to amplify it again but with primers that had overhangs with the backbone, in order to have an insert of about 100 bp. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The final concentrations of the purifications were all very low. I still did a DpnI digestion with 8 ul DNA and then a Gibson assembly but it did not work. As I had done only a 20 ul reaction of the PCR, I had no more Product left so I decided to do the PCR again but with a volume of 50 ul.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The final concentrations of the purifications were all very low. I still did a DpnI digestion with 8 ul DNA and then a Gibson assembly but it did not work. As I had done only a 20 ul reaction of the PCR, I had no more Product left so I decided to do the PCR again but with a volume of 50 ul.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><BR><BR></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><font size = "4"> Nanoparticles</font> <BR></ins></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>'''<del class="diffchange diffchange-inline">Nanoparticles</del>'<del class="diffchange diffchange-inline">''</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>''<ins class="diffchange diffchange-inline">ELISA-like assay</ins>''<ins class="diffchange diffchange-inline"><BR></ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Continuing with the ELISA-like assay.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Continuing with the ELISA-like assay.</div></td></tr>
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</table>GigiGoesGuguhttp://2013.igem.org/wiki/index.php?title=Team:EPF_Lausanne/Calendar/5_September_2013&diff=271014&oldid=prevLeabernier at 21:18, 2 October 20132013-10-02T21:18:50Z<p></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 21:18, 2 October 2013</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The final concentrations of the purifications were all very low. I still did a DpnI digestion with 8 ul DNA and then a Gibson assembly but it did not work. As I had done only a 20 ul reaction of the PCR, I had no more Product left so I decided to do the PCR again but with a volume of 50 ul.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The final concentrations of the purifications were all very low. I still did a DpnI digestion with 8 ul DNA and then a Gibson assembly but it did not work. As I had done only a 20 ul reaction of the PCR, I had no more Product left so I decided to do the PCR again but with a volume of 50 ul.</div></td></tr>
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</table>Leabernierhttp://2013.igem.org/wiki/index.php?title=Team:EPF_Lausanne/Calendar/5_September_2013&diff=255499&oldid=prevGigiGoesGugu at 18:51, 30 September 20132013-09-30T18:51:09Z<p></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 18:51, 30 September 2013</td>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">'''Purification of PCR products''' <BR></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">After PCR we purified the products with the qiagen Kit according to the manufacturers instructions. As the pH promoter from iGEM is only 30 bp it was removed during purification. So we decided to amplify it again but with primers that had overhangs with the backbone, in order to have an insert of about 100 bp. </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">The final concentrations of the purifications were all very low. I still did a DpnI digestion with 8 ul DNA and then a Gibson assembly but it did not work. As I had done only a 20 ul reaction of the PCR, I had no more Product left so I decided to do the PCR again but with a volume of 50 ul.</ins></div></td></tr>
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</table>GigiGoesGuguhttp://2013.igem.org/wiki/index.php?title=Team:EPF_Lausanne/Calendar/5_September_2013&diff=255491&oldid=prevGigiGoesGugu: Created page with "{{Template:EPFL2013Header}} Sensing <BR> '''PCR of the two pH sensitive Promoters and the constitutive promoter from iGEM''' <BR> We used the same program as the day before in ..."2013-09-30T18:48:43Z<p>Created page with "{{Template:EPFL2013Header}} Sensing <BR> '''PCR of the two pH sensitive Promoters and the constitutive promoter from iGEM''' <BR> We used the same program as the day before in ..."</p>
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Sensing <BR><br />
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'''PCR of the two pH sensitive Promoters and the constitutive promoter from iGEM''' <BR><br />
We used the same program as the day before in order to amplify the promoters. For the hya and the Cad promoters we put 3 min initial denaturation as they were contained in genomic DNA. Again the PCR was successful<br />
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