Team:EPF Lausanne/Calendar/6 August 2013

From 2013.igem.org

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'''Cell Surface Display'''
'''Cell Surface Display'''
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''First Gibson Transformation: ''  
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''Gibson Transformation: ''  
<br>Transformation of HB5α competent cells with ''INP_Strep-Dead'' gibson product previously obtained.  
<br>Transformation of HB5α competent cells with ''INP_Strep-Dead'' gibson product previously obtained.  
<br>Overnight incubation of the transformed cells in chloramphenicol paltes at 37°C.
<br>Overnight incubation of the transformed cells in chloramphenicol paltes at 37°C.
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<br>overnight Inoculation in LB + Amp medium of colonies from BBa_K283010 (05.08.10) ampicillin plates.
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Revision as of 21:53, 1 October 2013

Taxi.Coli: Smart Drug Delivery iGEM EPFL

Header

Cell Surface Display

Gibson Transformation:
Transformation of HB5α competent cells with INP_Strep-Dead gibson product previously obtained.
Overnight incubation of the transformed cells in chloramphenicol paltes at 37°C.


overnight Inoculation in LB + Amp medium of colonies from BBa_K283010 (05.08.10) ampicillin plates.