Team:EPF Lausanne/Calendar/6 August 2013


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Taxi.Coli: Smart Drug Delivery iGEM EPFL


Cell Surface Display

Gibson Transformation
-Transformation of DH5α competent cells with INP_Strep-Dead gibson product previously obtained.
-Overnight incubation of the transformed cells in chloramphenicol plates at 37°C.
-Overnight Inoculation in LB + Amp medium of colonies from BBa_K283010 (05.08.10) ampicillin plates.


Making Nanoparticles, 1st try
- Purification of the digested GPs.
- Measurement of the GPs+dye with Dynamic Light Scattering (DLS).
Results: good, we got some 10 nm particles and a few 100 nm. We hope they are nanoparticles and not artifacts.
- Started a new stock of GPs; made the gelatin solution, cross-linked with glutaraldehyde and stirred overnight.