Team:EPF Lausanne/Calendar/8 August 2013

From 2013.igem.org

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<font size = "4"> Cell Surface Display </font> <BR>
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''Streptavidin iGEM Cloning: ''
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<br> Gradient PCR of the Streptavidin BBa_K283010 received from iGEM registry with the following conditions: 68,70,72 °C ; mix without DMSO and mix with 3% and 5% DMSO.
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<br> Gel electrophoresis to visualise products.
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<br> The 72°C without DSMO showed least aspecific bands so we decided to go on with the larger amount PCR.
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<br> larger PCR of 50µl at 72°C without DMSO in the mix.
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<br> Gel Electrophoresis to verify product.
<font size = "4"> Sensing </font> <BR>
<font size = "4"> Sensing </font> <BR>

Revision as of 20:54, 1 October 2013

Taxi.Coli: Smart Drug Delivery iGEM EPFL

Header

Cell Surface Display

Streptavidin iGEM Cloning:
Gradient PCR of the Streptavidin BBa_K283010 received from iGEM registry with the following conditions: 68,70,72 °C ; mix without DMSO and mix with 3% and 5% DMSO.
Gel electrophoresis to visualise products.
The 72°C without DSMO showed least aspecific bands so we decided to go on with the larger amount PCR.
larger PCR of 50µl at 72°C without DMSO in the mix.
Gel Electrophoresis to verify product.

Sensing

Amplifying Plasmids
Trasformed DH5 alpha cells with the Plasmids containing parts BBa_I746908 (Arabinose promoter) & BBa_J23119 (pH promoter). These two parts are going to be used later in our experiemnts. The Cells