Team:EPF Lausanne/Calendar/8 August 2013

From 2013.igem.org

(Difference between revisions)
Line 10: Line 10:
<br> Gel Electrophoresis to verify product.
<br> Gel Electrophoresis to verify product.
-
<BR><BR>
+
<BR>
<font size = "4"> Sensing-Effector</font>  
<font size = "4"> Sensing-Effector</font>  
''Amplifying Plasmids'' <BR>
''Amplifying Plasmids'' <BR>
Trasformed DH5 alpha cells with the Plasmids containing parts BBa_I746908 (Arabinose promoter) & BBa_J23119 (pH promoter). These two parts are going to be used later in our experiemnts.
Trasformed DH5 alpha cells with the Plasmids containing parts BBa_I746908 (Arabinose promoter) & BBa_J23119 (pH promoter). These two parts are going to be used later in our experiemnts.
-
<BR>
+
<BR><BR>
<font size = "4"> Nanoparticles</font>  
<font size = "4"> Nanoparticles</font>  
-
''Making nanoparticules, 1st try'' <BR>
+
 
 +
''Making Nanoparticles, 1st try'' <BR>
<br>- Measurement of the new GPs with the DLS: we got no results.
<br>- Measurement of the new GPs with the DLS: we got no results.
<br>- Measurement of the food dye we used diluted in water: we got the same results as for our first measurement; i.e. some 10nm and a few 100nm particles. We can conclude we never had nanoparticles, the protocol we used did not work!  
<br>- Measurement of the food dye we used diluted in water: we got the same results as for our first measurement; i.e. some 10nm and a few 100nm particles. We can conclude we never had nanoparticles, the protocol we used did not work!  
-
 
{{Template:EPFL2013Footer}}
{{Template:EPFL2013Footer}}

Revision as of 20:58, 3 October 2013

Taxi.Coli: Smart Drug Delivery iGEM EPFL

Header

Cell Surface Display

Streptavidin iGEM Cloning
Gradient PCR of Streptavidin iGEM miniprep with the following conditions: 68,70,72 °C ; mix without DMSO and mix with 3% and 5% DMSO.
Gel electrophoresis to visualise products.
The 72°C without DSMO showed least aspecific bands so we decided to go on with the larger amount PCR.
larger PCR of 50µl at 72°C without DMSO in the mix.
Gel Electrophoresis to verify product.


Sensing-Effector

Amplifying Plasmids
Trasformed DH5 alpha cells with the Plasmids containing parts BBa_I746908 (Arabinose promoter) & BBa_J23119 (pH promoter). These two parts are going to be used later in our experiemnts.

Nanoparticles

Making Nanoparticles, 1st try

- Measurement of the new GPs with the DLS: we got no results.
- Measurement of the food dye we used diluted in water: we got the same results as for our first measurement; i.e. some 10nm and a few 100nm particles. We can conclude we never had nanoparticles, the protocol we used did not work!