Team:EPF Lausanne/Calendar/8 September 2013

From 2013.igem.org

(Difference between revisions)
(Created page with "{{Template:EPFL2013Header}} Sensing <BR> '''PCR of MMP9 insert and GelE insert''' <BR> I did the PCR of the MMP9 and the gelE insert which worked. I then purified it which gave...")
Line 1: Line 1:
{{Template:EPFL2013Header}}
{{Template:EPFL2013Header}}
-
Sensing <BR>
+
<font size = "4"> Sensing </font> <BR>
-
'''PCR of MMP9 insert and GelE insert''' <BR>
+
''PCR of MMP9 insert and GelE insert'' <BR>
-
I did the PCR of the MMP9 and the gelE insert which worked. I then purified it which gave me concentrations around 100ng/ul.  
+
-I did the PCR of the MMP9 and the gelE insert which worked. I then purified it which gave me concentrations around 100ng/ul.  
-
'''DpnI digest of MMP2 insert and MMP2 Backbone''' <BR>
+
''DpnI digest of MMP2 insert and MMP2 Backbone'' <BR>
After the PCR of the previous days I digested both the MMP2 insert and Backbone for the Gibson Assembly.
After the PCR of the previous days I digested both the MMP2 insert and Backbone for the Gibson Assembly.
-
 
+
''Gibson assembly for the MMP2+GFP construct'' <BR>
-
'''Gibson assembly for the MMP2+GFP construct''' <BR>
+
After the digest I did a Gibson assembly of the construct that contains the MMP2 gelatinase and superfolded GFP whose combined expression is induced by arabinose.
After the digest I did a Gibson assembly of the construct that contains the MMP2 gelatinase and superfolded GFP whose combined expression is induced by arabinose.
-
 
+
''Transfromation of cells with the Cad-GFP Gibson Assembly Product (Sensing construct)'' <BR>
-
'''Transfromation of cells with the Cad-GFP Gibson Assembly Product (Sensing construct)''' <BR>
+
I transformed Cells with one cad-sensing Construct. They effectively grew, meaning that they did contain some sort of resistance. This indicated that the gibson had worked.
I transformed Cells with one cad-sensing Construct. They effectively grew, meaning that they did contain some sort of resistance. This indicated that the gibson had worked.
{{Template:EPFL2013Footer}}
{{Template:EPFL2013Footer}}

Revision as of 16:40, 3 October 2013

Taxi.Coli: Smart Drug Delivery iGEM EPFL

Header

Sensing

PCR of MMP9 insert and GelE insert
-I did the PCR of the MMP9 and the gelE insert which worked. I then purified it which gave me concentrations around 100ng/ul.

DpnI digest of MMP2 insert and MMP2 Backbone
After the PCR of the previous days I digested both the MMP2 insert and Backbone for the Gibson Assembly.

Gibson assembly for the MMP2+GFP construct
After the digest I did a Gibson assembly of the construct that contains the MMP2 gelatinase and superfolded GFP whose combined expression is induced by arabinose.

Transfromation of cells with the Cad-GFP Gibson Assembly Product (Sensing construct)
I transformed Cells with one cad-sensing Construct. They effectively grew, meaning that they did contain some sort of resistance. This indicated that the gibson had worked.