http://2013.igem.org/wiki/index.php?title=Team:EPF_Lausanne/Next_steps&feed=atom&action=historyTeam:EPF Lausanne/Next steps - Revision history2024-03-28T19:44:17ZRevision history for this page on the wikiMediaWiki 1.16.5http://2013.igem.org/wiki/index.php?title=Team:EPF_Lausanne/Next_steps&diff=354801&oldid=prevSandelisa90: /* Cell surface display of Streptavidin */2013-10-28T23:57:52Z<p><span class="autocomment">Cell surface display of Streptavidin</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We actually used the time between the Jamboree in Lyon and the one at MIT, to clone the two plasmids, and did microscopy using an anti YFP antibody. Check out Cell surface display for further information. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We actually used the time between the Jamboree in Lyon and the one at MIT, to clone the two plasmids, and did microscopy using an anti YFP antibody. Check out Cell surface display for further information. </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The results were promising, but we would need to repeat the assay several times, <del class="diffchange diffchange-inline">and </del>improve the antibody <del class="diffchange diffchange-inline">concentrationa </del>and incubation time, for better images.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The results were promising, but we would need to <ins class="diffchange diffchange-inline">sequence verify our constructs and </ins>repeat the assay several times, improve the antibody <ins class="diffchange diffchange-inline">concentration </ins>and incubation time, for better images.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Additionally, we would have inquired which other proteins could be fused to INP and still be used to bind nanoparticles.<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Additionally, we would have inquired which other proteins could be fused to INP and still be used to bind nanoparticles.<br></div></td></tr>
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</table>Sandelisa90http://2013.igem.org/wiki/index.php?title=Team:EPF_Lausanne/Next_steps&diff=354770&oldid=prevSandelisa90: /* Cell surface display of Streptavidin */2013-10-28T23:56:32Z<p><span class="autocomment">Cell surface display of Streptavidin</span></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>We actually used the time between the Jamboree in Lyon and the one at MIT, to clone the two plasmids, and did microscopy using an anti YFP antibody. Check out Cell <del class="diffchange diffchange-inline">surfac </del>display for further information. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>We actually used the time between the Jamboree in Lyon and the one at MIT, to clone the two plasmids, and did microscopy using an anti YFP antibody. Check out Cell <ins class="diffchange diffchange-inline">surface </ins>display for further information. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The results were promising, but we would need to repeat the assay several times, and improve the antibody concentrationa and incubation time, for better images.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The results were promising, but we would need to repeat the assay several times, and improve the antibody concentrationa and incubation time, for better images.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Additionally, we would have inquired which other proteins could be fused to INP and still be used to bind nanoparticles.<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Additionally, we would have inquired which other proteins could be fused to INP and still be used to bind nanoparticles.<br></div></td></tr>
</table>Sandelisa90http://2013.igem.org/wiki/index.php?title=Team:EPF_Lausanne/Next_steps&diff=354097&oldid=prevMareikeapelt: /* Cell surface display of Streptavidin */2013-10-28T23:25:00Z<p><span class="autocomment">Cell surface display of Streptavidin</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Cell surface display of Streptavidin==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Cell surface display of Streptavidin==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We weren't able to prove that the fusion protein between Inp and Streptavidin was exported to the outer membrane. The next step would be to clone a plasmid that encodes a fusion protein between Inp, streptavidin and YFP. Then, we would be able to follow the same immunofluorescent staining protocol we successfully used to characterize the Biobrick BBa_K523013. We actually started this cloning strategy, but didn't have enough time to optimize the PCRs.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We weren't able to prove that the fusion protein between Inp and Streptavidin was exported to the outer membrane. The next step would be to clone a plasmid that encodes a fusion protein between Inp, streptavidin and YFP. Then, we would be able to follow the same immunofluorescent staining protocol we successfully used to characterize the Biobrick BBa_K523013. We actually started this cloning strategy, but didn't have enough time to optimize the PCRs.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">We actually used the time between the Jamboree in Lyon and the one at MIT, to clone the two plasmids, and did microscopy using an anti YFP antibody. Check out Cell surfac display for further information. </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">The results were promising, but we would need to repeat the assay several times, and improve the antibody concentrationa and incubation time, for better images.</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Additionally, we would have inquired which other proteins could be fused to INP and still be used to bind nanoparticles.<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Additionally, we would have inquired which other proteins could be fused to INP and still be used to bind nanoparticles.<br></div></td></tr>
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</table>Mareikeapelthttp://2013.igem.org/wiki/index.php?title=Team:EPF_Lausanne/Next_steps&diff=340792&oldid=prevLeabernier: /* What we would have done with 1 month more */2013-10-27T21:59:20Z<p><span class="autocomment">What we would have done with 1 month more</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Up until now, we succeeded to produce nanoparticles and to load them, to clone a pH sensitive promoter and to engineer a fusion protein between ice nucleation protein (INP) and streptavidin.<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Up until now, we succeeded to produce nanoparticles and to load them, to clone a pH sensitive promoter and to engineer a fusion protein between ice nucleation protein (INP) and streptavidin.<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>But we neither had the time to characterize our parts extensively nor to assemble the parts to finalize the Taxi.Coli.<br\></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>But we neither had the time to characterize our parts extensively nor to assemble the parts to finalize the Taxi.Coli.<br\></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>==What we would have done with 1 <del class="diffchange diffchange-inline">month </del>more==</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>==What we would have done with 1 more <ins class="diffchange diffchange-inline">month</ins>==</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Nanoparticles==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Nanoparticles==</div></td></tr>
</table>Leabernierhttp://2013.igem.org/wiki/index.php?title=Team:EPF_Lausanne/Next_steps&diff=338993&oldid=prevLeabernier at 18:13, 27 October 20132013-10-27T18:13:35Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Nanoparticles==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Nanoparticles==</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>This part of our project is rather advanced, but there are still some assays that we would have loved to make. For example, to test if and how fast the nanoparticles are digested by GelatinaseE and Matrix metalloprotease 2 (MMP2), we would have performed a fluorescence release assay using a plate reader. First, we would have used commercial enzymes to <del class="diffchange diffchange-inline">ckeck </del>that the assay works and maybe adjust the protocol, later with the enzymes produced by the E.coli transformed with the effector plasmid.<br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>This part of our project is rather advanced, but there are still some assays that we would have loved to make. For example, to test if and how fast the nanoparticles are digested by GelatinaseE and Matrix metalloprotease 2 (MMP2), we would have performed a fluorescence release assay using a plate reader. First, we would have used commercial enzymes to <ins class="diffchange diffchange-inline">check </ins>that the assay works and maybe adjust the protocol, later with the enzymes produced by the E.coli transformed with the effector plasmid.<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Eventually, we would have also tried to load the nanoparticles with an actual drug.<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Eventually, we would have also tried to load the nanoparticles with an actual drug.<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
</table>Leabernierhttp://2013.igem.org/wiki/index.php?title=Team:EPF_Lausanne/Next_steps&diff=310765&oldid=prevLeabernier: /* Taxi.Coli */2013-10-05T00:35:12Z<p><span class="autocomment">Taxi.Coli</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Taxi.Coli==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Taxi.Coli==</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Once all the subparts worked, we would clone one Biobrick made out of our promoter and our gelatinase gene in one backbone. This backbone would then contain a different antibiotic resistance than our Cell-surface display plasmid so we could <del class="diffchange diffchange-inline">cotransform </del>bacteria with the plasmid responsible for sensing/effector and the plasmid that encodes the Inp-streptavidin fusion protein. Then we would bind the nanoparticles to those Taxi.Coli.<br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Once all the subparts worked, we would clone one Biobrick made out of our promoter and our gelatinase gene in one backbone. This backbone would then contain a different antibiotic resistance than our Cell-surface display plasmid so <ins class="diffchange diffchange-inline">that </ins>we could <ins class="diffchange diffchange-inline">co-transform </ins>bacteria with the plasmid responsible for sensing/effector and the plasmid that encodes the Inp-streptavidin fusion protein. Then<ins class="diffchange diffchange-inline">, </ins>we would bind the nanoparticles to those Taxi.Coli <ins class="diffchange diffchange-inline">bacteria</ins>.<br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The last step to complete the proof of principle we wanted to provide<del class="diffchange diffchange-inline">, </del>is to characterize extensively <del class="diffchange diffchange-inline">these </del>final version of Taxi.Coli smart drug delivery.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The last step to complete the proof of principle we wanted to provide is to characterize extensively <ins class="diffchange diffchange-inline">this </ins>final version of Taxi.Coli smart drug delivery.</div></td></tr>
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</table>Leabernierhttp://2013.igem.org/wiki/index.php?title=Team:EPF_Lausanne/Next_steps&diff=310709&oldid=prevLeabernier: /* Cell surface display of Streptavidin */2013-10-05T00:33:36Z<p><span class="autocomment">Cell surface display of Streptavidin</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Cell surface display of Streptavidin==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Cell surface display of Streptavidin==</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>We weren't able to <del class="diffchange diffchange-inline">proove </del>that the fusion protein between Inp and Streptavidin was exported to the outer membrane. The next step would be to clone a plasmid that encodes a fusion protein between Inp, streptavidin and YFP. Then, we would be able to follow the same immunofluorescent staining protocol we successfully used to characterize the Biobrick BBa_K523013. We actually started this cloning strategy, but didn't have enough time to optimize the PCRs.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>We weren't able to <ins class="diffchange diffchange-inline">prove </ins>that the fusion protein between Inp and Streptavidin was exported to the outer membrane. The next step would be to clone a plasmid that encodes a fusion protein between Inp, streptavidin and YFP. Then, we would be able to follow the same immunofluorescent staining protocol we successfully used to characterize the Biobrick BBa_K523013. We actually started this cloning strategy, but didn't have enough time to optimize the PCRs.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Additionally, we would have inquired which other proteins could be fused to INP and still be used to bind nanoparticles.<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Additionally, we would have inquired which other proteins could be fused to INP and still be used to bind nanoparticles.<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
</table>Leabernierhttp://2013.igem.org/wiki/index.php?title=Team:EPF_Lausanne/Next_steps&diff=310704&oldid=prevLeabernier: /* Sensing/Effector */2013-10-05T00:33:23Z<p><span class="autocomment">Sensing/Effector</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Sensing/Effector==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Sensing/Effector==</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>We <del class="diffchange diffchange-inline">succesfully </del>cloned a pH sensitive promoter, and <del class="diffchange diffchange-inline">proofed </del>its functionality, but with a bit more time we would have characterized it in more <del class="diffchange diffchange-inline">detail </del>and would have been able to find its optimal expression conditions. For example we would have repeated the plate reader experiments with a wider range of pH values and even with different buffers.<br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>We <ins class="diffchange diffchange-inline">successfully </ins>cloned a pH sensitive promoter, and <ins class="diffchange diffchange-inline">proved </ins>its functionality, but<ins class="diffchange diffchange-inline">, </ins>with a bit more time<ins class="diffchange diffchange-inline">, </ins>we would have characterized it in more <ins class="diffchange diffchange-inline">details </ins>and would have been able to find its optimal expression conditions. For example<ins class="diffchange diffchange-inline">, </ins>we would have repeated the plate reader experiments with a wider range of pH values and even with different buffers.<br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Furthermore we would have transformed another strain of E.coli (MG1655) from which the promoters (hya and cad) originate with the <del class="diffchange diffchange-inline"> </del>plasmid. This would assure that the necessary transcription factors and activators are present and can initiate transcription of the superfolded GFP.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Furthermore<ins class="diffchange diffchange-inline">, </ins>we would have transformed another strain of E.coli (MG1655) from which the promoters (hya and cad) originate with the plasmid. This would assure that the necessary transcription factors and activators are present and can initiate transcription of the superfolded GFP.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>In parallel we would have restarted the cloning of the hya and <del class="diffchange diffchange-inline">cadpromoters </del>with slight variations of sequences, to check for a better inducible system.<br><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>In parallel<ins class="diffchange diffchange-inline">, </ins>we would have restarted the cloning of the hya and <ins class="diffchange diffchange-inline">cad promoters </ins>with slight variations of sequences, to check for a better inducible system.<br><br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The effector part wasn't very <del class="diffchange diffchange-inline">succesful</del>, but we would repeat the purification assay of Gelatinase E and MMP2 to make sure that our negative results aren't due to <del class="diffchange diffchange-inline">human </del>error. <br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The effector part wasn't very <ins class="diffchange diffchange-inline">successful</ins>, but we would repeat the purification assay of Gelatinase E and MMP2 to make sure that our negative results aren't due to <ins class="diffchange diffchange-inline">manipulations </ins>error. <br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Additionally we would have designed new primers to have a fusion protein between the enzymes and GFP which would facilitate to <del class="diffchange diffchange-inline">proof </del>its production.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Additionally<ins class="diffchange diffchange-inline">, </ins>we would have designed new primers to have a fusion protein between the enzymes and GFP which would facilitate to <ins class="diffchange diffchange-inline">prove </ins>its production.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Eventually <del class="diffchange diffchange-inline"> </del>we would clone the sensing promoter and the effector enzymes together into one plasmid. Thus having a plasmid that produces the nanoparticle degrading enzyme when triggered by a pH change, as <del class="diffchange diffchange-inline">was </del>the original idea.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Eventually<ins class="diffchange diffchange-inline">, </ins>we would clone the sensing promoter and the effector enzymes together into one plasmid. Thus having a plasmid that produces the nanoparticle degrading enzyme when triggered by a pH change, as <ins class="diffchange diffchange-inline">in </ins>the original idea.</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Taxi.Coli==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Taxi.Coli==</div></td></tr>
</table>Leabernierhttp://2013.igem.org/wiki/index.php?title=Team:EPF_Lausanne/Next_steps&diff=310609&oldid=prevLeabernier: /* Cell surface display of Streptavidin */2013-10-05T00:30:39Z<p><span class="autocomment">Cell surface display of Streptavidin</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Cell surface display of Streptavidin==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Cell surface display of Streptavidin==</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>We weren't able to <del class="diffchange diffchange-inline">proof </del>that the fusion protein between Inp and Streptavidin was exported to the outer membrane. The next step would be to clone a plasmid that encodes a fusion protein between Inp,streptavidin and YFP. Then we would be able to follow the same immunofluorescent staining protocol we <del class="diffchange diffchange-inline">succesfully </del>used to <del class="diffchange diffchange-inline">characterized </del>the Biobrick BBa_K523013. We actually started this cloning strategy, but didn't have enough time to optimize the PCRs.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>We weren't able to <ins class="diffchange diffchange-inline">proove </ins>that the fusion protein between Inp and Streptavidin was exported to the outer membrane. The next step would be to clone a plasmid that encodes a fusion protein between Inp, streptavidin and YFP. Then<ins class="diffchange diffchange-inline">, </ins>we would be able to follow the same immunofluorescent staining protocol we <ins class="diffchange diffchange-inline">successfully </ins>used to <ins class="diffchange diffchange-inline">characterize </ins>the Biobrick BBa_K523013. We actually started this cloning strategy, but didn't have enough time to optimize the PCRs.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Additionally we would have <del class="diffchange diffchange-inline">inquiered </del>which other proteins could be fused to INP and still be used to bind nanoparticles.<br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Additionally<ins class="diffchange diffchange-inline">, </ins>we would have <ins class="diffchange diffchange-inline">inquired </ins>which other proteins could be fused to INP and still be used to bind nanoparticles.<br></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Sensing/Effector==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Sensing/Effector==</div></td></tr>
</table>Leabernierhttp://2013.igem.org/wiki/index.php?title=Team:EPF_Lausanne/Next_steps&diff=310559&oldid=prevLeabernier: /* Nanoparticles */2013-10-05T00:29:26Z<p><span class="autocomment">Nanoparticles</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Nanoparticles==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Nanoparticles==</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>This part of our project is rather advanced, but there are still some assays that we would have loved to make. For example to test if and how fast the nanoparticles are digested by GelatinaseE and <del class="diffchange diffchange-inline">Matrixmetalloprotease </del>2 (MMP2) we would have performed a <del class="diffchange diffchange-inline">fluorescent </del>release assay using a plate reader. First we would have used commercial enzymes<del class="diffchange diffchange-inline">, </del>to <del class="diffchange diffchange-inline">try if </del>the assay works and maybe adjust the protocol, later with the enzymes produced by the E.coli transformed with the effector plasmid.<br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>This part of our project is rather advanced, but there are still some assays that we would have loved to make. For example<ins class="diffchange diffchange-inline">, </ins>to test if and how fast the nanoparticles are digested by GelatinaseE and <ins class="diffchange diffchange-inline">Matrix metalloprotease </ins>2 (MMP2)<ins class="diffchange diffchange-inline">, </ins>we would have performed a <ins class="diffchange diffchange-inline">fluorescence </ins>release assay using a plate reader. First<ins class="diffchange diffchange-inline">, </ins>we would have used commercial enzymes to <ins class="diffchange diffchange-inline">ckeck that </ins>the assay works and maybe adjust the protocol, later with the enzymes produced by the E.coli transformed with the effector plasmid.<br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Eventually we would have also tried to load the nanoparticles with an actual drug.<br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Eventually<ins class="diffchange diffchange-inline">, </ins>we would have also tried to load the nanoparticles with an actual drug.<br></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Cell surface display of Streptavidin==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Cell surface display of Streptavidin==</div></td></tr>
</table>Leabernier