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Up until now we succeded • Producing Nanoparticles and loading them • Clone a pH sensitive promoter • Engineer a fusion protein between ice nucleation protein and Streptavidin But we weren’t able to characterize and assemble the parts completely.
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What we would have done with more time:
Nanoparticles
• • •
Cell surface display of Streptavidin
• Cloned a plasmid that encodes a fusion protein INP-Streptavidin-YFP and one INP-YFP-Streptavidin. Then we would have been able to use the exact same protocol we used to characterize the Biobrick BBa_K523013, to proof surface localization. • •
Sensing/Effector
• • •
Device
• Cloned one of our Biobricks in a backbone that contains another resistance than chloramphenicol to cotransfect bacteria with the Plasmids responsible for Sensing/Effector and the Plasmid used to connect the Nanoparticles. • •
