http://2013.igem.org/wiki/index.php?title=Team:EPF_Lausanne/Safety&feed=atom&action=historyTeam:EPF Lausanne/Safety - Revision history2024-03-28T20:32:36ZRevision history for this page on the wikiMediaWiki 1.16.5http://2013.igem.org/wiki/index.php?title=Team:EPF_Lausanne/Safety&diff=316636&oldid=prevLeabernier: /* Risk assessment and safety: TAXI.COLI: smart drug delivery */2013-10-05T03:37:27Z<p><span class="autocomment">Risk assessment and safety: TAXI.COLI: smart drug delivery</span></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>As you might know iGEM is not only about working in a lab, pipetting in lab coats and doing cool stuff with bacteria. iGEM is also about asking yourself the good questions when it comes to the implications that our project could have on our peers and on our environment. The team decided that evaluating our project with a risk assessment was a good way <del class="diffchange diffchange-inline">of bringing </del>into focus what could be the impacts of our project on co-workers, peers and on nature. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>As you might know iGEM is not only about working in a lab, pipetting in lab coats and doing cool stuff with bacteria. iGEM is also about asking yourself the good questions when it comes to the implications that our project could have on our peers and on our environment. The team decided that evaluating our project with a risk assessment was a good way <ins class="diffchange diffchange-inline">to bring </ins>into focus what could be the impacts of our project on co-workers, peers and on nature. </div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Overview: Our project consists mainly <del class="diffchange diffchange-inline">of </del>engineering E.coli in order to transport nanoparticles filled with drugs to a specific site of action where the particles would be digested by <del class="diffchange diffchange-inline">a release of </del>gelatinase, thus allowing the drugs to enter this particular site where its action is required. An application of such a device could be <del class="diffchange diffchange-inline">atreatment </del>of colon cancer. E.coli being already a part of our gut flora, we thought it could be a good chassis to begin the experiments with. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Overview: Our project consists mainly <ins class="diffchange diffchange-inline">in </ins>engineering E.coli in order to transport nanoparticles filled with drugs to a specific site of action where the particles would be digested by <ins class="diffchange diffchange-inline">released </ins>gelatinase, thus allowing the drugs to enter this particular site where its action is required. An application of such a device could be <ins class="diffchange diffchange-inline">treatment </ins>of colon cancer. E.coli being already a part of our gut flora, we thought it could be a good chassis to begin the experiments with. </div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:Team-EPF-Lausanne-gallery22.jpeg|thumb|center|500px|Figure 1: safety in our labs]] </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:Team-EPF-Lausanne-gallery22.jpeg|thumb|center|500px|Figure 1: safety in our labs]] </div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''a. Risks to the safety and health of team members or others working in the lab?'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''a. Risks to the safety and health of team members or others working in the lab?'''</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The biological materials <del class="diffchange diffchange-inline">we’re </del>working with in the lab are thought to be as safe as possible for all the team and co-workers. Indeed, our plan since the beginning of the iGEM project is to build a project which couldn’t raise any particular safety issues for us, the students, but also for our co-workers in the lab. We are particularly interested in designing a project that relies on simple ideas but which is still innovative, original and challenging. Besides, our team followed some specific biosafety lectures given by Stéphane Karlen our school safety coordinator<del class="diffchange diffchange-inline">. We were particularly careful on wearing permanently our lab coats and gloves in the lab. Indeed working with RG1 biological material doesn’t mean that no precautions should be taken</del>. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The biological materials <ins class="diffchange diffchange-inline">we are </ins>working with in the lab are thought to be as safe as possible for all the team and co-workers. Indeed, our plan since the beginning of the iGEM project is to build a project which couldn’t raise any particular safety issues for us, the students, but also for our co-workers in the lab. We are particularly interested in designing a project that relies on simple ideas<ins class="diffchange diffchange-inline">, </ins>but which is still innovative, original and challenging. Besides, our team followed some specific biosafety lectures given by Stéphane Karlen<ins class="diffchange diffchange-inline">, </ins>our school safety coordinator.</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''b. Risks to the safety and health of the general public, if released by design or by accident?'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''b. Risks to the safety and health of the general public, if released by design or by accident?'''</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">Risk considerations are different regarding what part of the population could be affected. For example </del>in <del class="diffchange diffchange-inline">our labs the populations is mainly young and do not have daily contact with young kids, babies or elder</del>. <del class="diffchange diffchange-inline">The general public is not the same population than in </del>a <del class="diffchange diffchange-inline">lab</del>. <del class="diffchange diffchange-inline">This is why we took special care in leaving our lab coats in </del>the <del class="diffchange diffchange-inline">lab, </del>not <del class="diffchange diffchange-inline">touching the doorknob with gloves and always closing the doors</del>. Furthermore the lab is a confined environment and it is organized in a way that no biological material could get out. Let’s imagine some biological material could get out of the lab<del class="diffchange diffchange-inline">, it </del>still <del class="diffchange diffchange-inline">could </del>not cause any arm to the general population since the quantities of bacteria <del class="diffchange diffchange-inline">we’re </del>working with are very small and also E.<del class="diffchange diffchange-inline">Coli </del>K12 that we are using is not armful nor toxic and is a lab strain<del class="diffchange diffchange-inline">; Meaning </del>it <del class="diffchange diffchange-inline">can’t </del>survive for a long time out of the lab. Furthermore our labs are restricted to visits.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Our project would require some improvements </ins>in <ins class="diffchange diffchange-inline">order for Taxi</ins>.<ins class="diffchange diffchange-inline">Coli to be used as </ins>a <ins class="diffchange diffchange-inline">drug delivery system</ins>. <ins class="diffchange diffchange-inline">These improvements are detailed below. However, once they are made, </ins>the <ins class="diffchange diffchange-inline">designed release of this drug delivery system should </ins>not <ins class="diffchange diffchange-inline">bring any safety issue</ins>.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Furthermore the lab is a confined environment and it is organized in a way that no biological material could get out. Let’s imagine <ins class="diffchange diffchange-inline">that </ins>some biological material could get out of the lab<ins class="diffchange diffchange-inline">. It could </ins>still not cause any arm to the general population<ins class="diffchange diffchange-inline">, </ins>since the quantities of bacteria <ins class="diffchange diffchange-inline">we are </ins>working with are very small<ins class="diffchange diffchange-inline">, </ins>and also E.<ins class="diffchange diffchange-inline">coli </ins>K12 that we are using is not armful nor toxic and is a lab strain<ins class="diffchange diffchange-inline">, meaning </ins>it <ins class="diffchange diffchange-inline">cannot </ins>survive for a long time out of the lab. Furthermore<ins class="diffchange diffchange-inline">, </ins>our labs are restricted to visits.</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''c. Risks to the environment, if released by design or by accident?'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''c. Risks to the environment, if released by design or by accident?'''</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The main problem that exists regarding the environment is resistance to antibiotics. Bacterial conjugation is the horizontal transfer of genetic material by direct cell-to-cell contact or by a bridge-like connection between <del class="diffchange diffchange-inline">two </del>cells. This could happen when engineered bacteria containing plasmids resistant to antibiotics are released in the environment thus exchanging their genetic material with wild-type bacteria. Therefore providing antibiotic resistance to bacteria that originally are not resistant. A second potential risk is that the quantity of bacteria released in the environment may disrupt the environmental balance. However we are working with really small quantities of bacteria so the second potential risk is not likely to happen. Still for the antibiotic resistance some precautions <del class="diffchange diffchange-inline">are necessary </del>to be undertaken. For example, our entire waste is first inactivated and then burned to prevent any release in the environment by our own waste treatment company in our university. <del class="diffchange diffchange-inline"> </del>All <del class="diffchange diffchange-inline">our used </del>material is autoclaved and sterilized. <del class="diffchange diffchange-inline"> </del>Furthermore we are working with E.coli DH5alpha (K12), which is a lab strain that wouldn’t survive in the environment.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The main problem that exists regarding the environment is resistance to antibiotics. Bacterial conjugation is the horizontal transfer of genetic material by direct cell-to-cell contact or by a bridge-like connection between cells. This could happen when engineered bacteria containing plasmids resistant to antibiotics are released in the environment<ins class="diffchange diffchange-inline">, </ins>thus exchanging their genetic material with wild-type bacteria. Therefore<ins class="diffchange diffchange-inline">, </ins>providing antibiotic resistance to bacteria that originally are not resistant. A second potential risk is that the quantity of bacteria released in the environment may disrupt the environmental balance. However we are working with really small quantities of bacteria<ins class="diffchange diffchange-inline">, </ins>so <ins class="diffchange diffchange-inline">that </ins>the second potential risk is not likely to happen. Still<ins class="diffchange diffchange-inline">, </ins>for the antibiotic resistance<ins class="diffchange diffchange-inline">, </ins>some precautions <ins class="diffchange diffchange-inline">need </ins>to be undertaken. For example, our entire waste is first inactivated and then burned to prevent any release in the environment by our own waste treatment company in our university. All <ins class="diffchange diffchange-inline">the </ins>material <ins class="diffchange diffchange-inline">we use </ins>is autoclaved and sterilized. Furthermore<ins class="diffchange diffchange-inline">, </ins>we are working with E.coli DH5alpha (K12), which is a lab strain that wouldn’t survive in the environment.</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''d. Risks to security through malicious misuse by individuals, groups, or countries?'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''d. Risks to security through malicious misuse by individuals, groups, or countries?'''</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Our labs are under constant control thus preventing any misbehavior or <del class="diffchange diffchange-inline">intend </del>to cause arm. <del class="diffchange diffchange-inline"> </del>Cameras, security agents and co-workers are on constant vigilance. Lab access is restricted at night and after working hours. Our main concern when designing our iGEM project was safety. We wanted to stick generally to RG1 organisms showing that great things can be accomplished also being respectful <del class="diffchange diffchange-inline">of </del>the environment and people. Even in malicious hands our project couldn’t cause arm or cause a risk to the individuals, groups, countries or environment because it is designed to be safe since <del class="diffchange diffchange-inline">the beginning of </del>its creation.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Our labs are under constant control thus preventing any misbehavior or <ins class="diffchange diffchange-inline">attempt </ins>to cause arm. Cameras, security agents and co-workers are on constant vigilance. Lab access is restricted at night and after working hours. Our main concern when designing our iGEM project was safety. We wanted to stick generally to RG1 organisms showing that great things can be accomplished also being respectful <ins class="diffchange diffchange-inline">to </ins>the environment and <ins class="diffchange diffchange-inline">to </ins>people. Even in malicious hands<ins class="diffchange diffchange-inline">, </ins>our project couldn’t cause arm or cause a risk to the individuals, groups, countries or environment<ins class="diffchange diffchange-inline">, </ins>because it is designed to be safe since its creation.</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''2: If your project moved from a small-scale lab study to become widely used as a commercial/industrial product, what new risks might arise? (Consider the different categories of risks that are listed in parts a-d of the previous question). Also, what risks might arise if the knowledge you generate or the methods you develop became widely available?'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''2: If your project moved from a small-scale lab study to become widely used as a commercial/industrial product, what new risks might arise? (Consider the different categories of risks that are listed in parts a-d of the previous question). Also, what risks might arise if the knowledge you generate or the methods you develop became widely available?'''</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>If our project moved from a small case study to a commercial product <del class="diffchange diffchange-inline">maybe </del>the measures regarding the waste treatment should be even more stringent to <del class="diffchange diffchange-inline">be </del>sure a high quantity of antibiotic resistance bacteria is not released in the environment. Also when treating a high concentration of bacteria in the lab, the co-workers should be particularly cautious when handling them <del class="diffchange diffchange-inline">being careful not to inhale</del>. <del class="diffchange diffchange-inline"> </del>Although E.<del class="diffchange diffchange-inline">Coli </del>K12 is not risky for humans it is always better to be more careful when handling bigger quantities of biological material and even more cautious regarding the waste treatment. Although quantity shouldn’t have an impact on proper behavior in the lab. Working with small quantities of RG1 in BSL1 labs doesn’t mean one shouldn’t have an appropriate, cautious and respectful behavior towards co-workers, environment and general public. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>If our project moved from a small case study to a commercial product<ins class="diffchange diffchange-inline">, </ins>the measures regarding the waste treatment should be even more stringent to <ins class="diffchange diffchange-inline">make </ins>sure a high quantity of antibiotic resistance bacteria is not released in the environment. Also<ins class="diffchange diffchange-inline">, </ins>when treating a high concentration of bacteria in the lab, the co-workers should be particularly cautious when handling them. Although E.<ins class="diffchange diffchange-inline">coli </ins>K12 is not risky for humans<ins class="diffchange diffchange-inline">, </ins>it is always better to be more careful when handling bigger quantities of biological material and even more cautious regarding the waste treatment. Although quantity shouldn’t have an impact on proper behavior in the lab. Working with small quantities of RG1 in BSL1 labs doesn’t mean one shouldn’t have an appropriate, cautious and respectful behavior towards co-workers, environment and general public. </div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''3: Does your project include any design features to address safety risks?''' </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''3: Does your project include any design features to address safety risks?''' </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Our project <del class="diffchange diffchange-inline">at the moment </del>doesn’t contain any feature to address safety risks as we are working on a proof of principle project. However we suggest several possibilities to address safety risks considering these two different cases:</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Our project doesn’t contain any feature to address safety risks <ins class="diffchange diffchange-inline">yet, </ins>as we are working on a proof of principle project. However<ins class="diffchange diffchange-inline">, </ins>we suggest several possibilities to address safety risks considering these two different cases:</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-Assuming our project could become widely used as a commercial medical application.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-Assuming our project could become widely used as a commercial medical application.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-In case of accidental dissemination in the environment. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-In case of accidental dissemination in the environment. </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>As a design feature to prevent any arm our project could cause in dissemination, we imagined engineering our chassis with an artificial amino acid<del class="diffchange diffchange-inline">; </del>thus preventing it from surviving out of an environment that is not containing the artificial amino acid. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>As a design feature to prevent any arm our project could cause in dissemination, we imagined engineering our chassis with an artificial amino acid<ins class="diffchange diffchange-inline">, </ins>thus preventing it from surviving out of an environment that is not containing the artificial amino acid. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In case: </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In case: </div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>a) Our project becomes a medical application: the patient ingests pills containing <del class="diffchange diffchange-inline">an </del>artificial amino acid during the whole treatment with the engineered E.coli. When the treatment ends the patient stops ingesting the artificial amino acid thus not allowing the engineered E.<del class="diffchange diffchange-inline">Coli </del>to survive in the colon anymore. We can also think of a way of treating, inactivating or burning the biological waste. The better way would still be to confine the patient during the whole treatment in the hospital so the <del class="diffchange diffchange-inline">waist </del>could be directly treated by the hospital itself.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>a) Our project becomes a medical application: the patient ingests pills containing <ins class="diffchange diffchange-inline">the </ins>artificial amino acid during the whole treatment with the engineered E.coli. When the treatment ends<ins class="diffchange diffchange-inline">, </ins>the patient stops ingesting the artificial amino acid<ins class="diffchange diffchange-inline">, </ins>thus not allowing the engineered E.<ins class="diffchange diffchange-inline">coli </ins>to survive in the colon anymore. We can also think of a way of treating, inactivating or burning the biological waste<ins class="diffchange diffchange-inline">. Another option would be to implement an induced apoptosis mechanism</ins>. The better way would still be to confine the patient during the whole treatment in the hospital so the <ins class="diffchange diffchange-inline">waste </ins>could be directly treated by the hospital itself.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>b) Our engineered E.coli are disseminated by accident in the environment: The chassis we used for our project as a proof of principle chassis is not suitable for medical application or to survive in the environment since it is a lab strain named <del class="diffchange diffchange-inline">DH5alpha </del>of E.<del class="diffchange diffchange-inline">Coli </del>K12. This strain is not able to survive neither in the environment nor in the human body. Furthermore the same mechanism as before can be also applied to the environment. Let’s assume our artificial amino acid engineered E.Coli is disseminated in the environment. The bacteria won’t survive considering the artificial amino acids are not present in the environment. The <del class="diffchange diffchange-inline">engineered E.coli </del>would <del class="diffchange diffchange-inline">then die after a couple of days</del>. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>b) Our engineered E.coli are disseminated by accident in the environment: The chassis we used for our project as a proof of principle chassis is not suitable for medical application or to survive in the environment<ins class="diffchange diffchange-inline">, </ins>since it is a lab strain named <ins class="diffchange diffchange-inline">DH5-alpha </ins>of E.<ins class="diffchange diffchange-inline">coli </ins>K12. This strain is not able to survive neither in the environment nor in the human body. Furthermore<ins class="diffchange diffchange-inline">, </ins>the same mechanism as before can be also applied to the environment. Let’s assume our artificial amino acid engineered E.Coli is disseminated in the environment. The bacteria won’t survive considering the artificial amino acids are not present in the environment. The <ins class="diffchange diffchange-inline">option with an induced apoptosis mechanism </ins>would <ins class="diffchange diffchange-inline">also prevent spreading in the environment</ins>.</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Since this is only <del class="diffchange diffchange-inline">assumption </del>and suggestions concerning the features addressing safety risks<del class="diffchange diffchange-inline">; </del>it would be very important to <del class="diffchange diffchange-inline">have </del>in mind the safety assessment we explored above and the safety precautions we presented. <del class="diffchange diffchange-inline">Though it </del>would be interesting to test it maybe next year for iGEM 2014 competition?</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Since this is only <ins class="diffchange diffchange-inline">assumptions </ins>and suggestions concerning the features addressing safety risks<ins class="diffchange diffchange-inline">, </ins>it would be very important to <ins class="diffchange diffchange-inline">keep </ins>in mind the safety assessment we explored above and the safety precautions we presented. <ins class="diffchange diffchange-inline">It </ins>would be interesting to test it maybe next year for iGEM 2014 competition?</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
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</table>Leabernierhttp://2013.igem.org/wiki/index.php?title=Team:EPF_Lausanne/Safety&diff=315976&oldid=prevGigiGoesGugu: /* Risk assessment and safety: TAXI.COLI: smart drug delivery */2013-10-05T03:18:02Z<p><span class="autocomment">Risk assessment and safety: TAXI.COLI: smart drug delivery</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''a. Risks to the safety and health of team members or others working in the lab?'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''a. Risks to the safety and health of team members or others working in the lab?'''</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The biological materials we’re working with in the lab are <del class="diffchange diffchange-inline">especially </del>thought to be as safe as possible for all the team and co-workers. Indeed, our plan since the beginning of the iGEM project is to build a project which couldn’t raise any particular safety issues for us, the students, but also for our co-workers in the lab. We are particularly interested in designing a project that relies on simple ideas but which is still innovative, original and challenging. Besides our team followed some specific biosafety lectures given by Stéphane Karlen our school safety coordinator. We were particularly careful on wearing permanently our lab coats and gloves in the lab. Indeed working with RG1 biological material doesn’t mean that no precautions should be taken. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The biological materials we’re working with in the lab are thought to be as safe as possible for all the team and co-workers. Indeed, our plan since the beginning of the iGEM project is to build a project which couldn’t raise any particular safety issues for us, the students, but also for our co-workers in the lab. We are particularly interested in designing a project that relies on simple ideas but which is still innovative, original and challenging. Besides<ins class="diffchange diffchange-inline">, </ins>our team followed some specific biosafety lectures given by Stéphane Karlen our school safety coordinator. We were particularly careful on wearing permanently our lab coats and gloves in the lab. Indeed working with RG1 biological material doesn’t mean that no precautions should be taken. </div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''b. Risks to the safety and health of the general public, if released by design or by accident?'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''b. Risks to the safety and health of the general public, if released by design or by accident?'''</div></td></tr>
</table>GigiGoesGuguhttp://2013.igem.org/wiki/index.php?title=Team:EPF_Lausanne/Safety&diff=315923&oldid=prevGigiGoesGugu: /* Risk assessment and safety: TAXI.COLI: smart drug delivery */2013-10-05T03:15:51Z<p><span class="autocomment">Risk assessment and safety: TAXI.COLI: smart drug delivery</span></p>
<table style="background-color: white; color:black;">
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>As you might know iGEM is not only about working in a lab, pipetting in lab coats and doing cool stuff with bacteria. iGEM is also about asking yourself the good questions when it comes to the implications that our project could have on our peers and on our environment. The team decided that evaluating our project with a risk assessment was a good way of bringing into focus what could be the impacts of our project on co-workers, peers and on nature. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>As you might know iGEM is not only about working in a lab, pipetting in lab coats and doing cool stuff with bacteria. iGEM is also about asking yourself the good questions when it comes to the implications that our project could have on our peers and on our environment. The team decided that evaluating our project with a risk assessment was a good way of bringing into focus what could be the impacts of our project on co-workers, peers and on nature. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Overview: Our project consists mainly <del class="diffchange diffchange-inline">on </del>engineering E.coli in order to transport nanoparticles filled with <del class="diffchange diffchange-inline">drug </del>to a specific site of action where the particles would be digested by a release of gelatinase, thus allowing the <del class="diffchange diffchange-inline">drug </del>to enter this particular site where its action is required. An application of such a device could be <del class="diffchange diffchange-inline">the treatment </del>of colon cancer. E.coli being already a part of our gut flora, we thought it could be a good chassis to begin the experiments with. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Overview: Our project consists mainly <ins class="diffchange diffchange-inline">of </ins>engineering E.coli in order to transport nanoparticles filled with <ins class="diffchange diffchange-inline">drugs </ins>to a specific site of action where the particles would be digested by a release of gelatinase, thus allowing the <ins class="diffchange diffchange-inline">drugs </ins>to enter this particular site where its action is required. An application of such a device could be <ins class="diffchange diffchange-inline">atreatment </ins>of colon cancer. E.coli being already a part of our gut flora, we thought it could be a good chassis to begin the experiments with. </div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:Team-EPF-Lausanne-gallery22.jpeg|thumb|center|500px|Figure 1: safety in our labs]] </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:Team-EPF-Lausanne-gallery22.jpeg|thumb|center|500px|Figure 1: safety in our labs]] </div></td></tr>
</table>GigiGoesGuguhttp://2013.igem.org/wiki/index.php?title=Team:EPF_Lausanne/Safety&diff=303298&oldid=prevLeabernier: /* Risk assessment and safety: TAXI.COLI: smart drug delivery */2013-10-04T20:51:32Z<p><span class="autocomment">Risk assessment and safety: TAXI.COLI: smart drug delivery</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>As you might know iGEM is not only about working in a lab, pipetting in lab coats and doing cool stuff with bacteria. iGEM is also about asking yourself the good questions when it comes to the implications that our project could have on our peers and on our environment. The team decided that evaluating our project with a risk assessment was a good way of bringing into focus what could be the impacts of our project on co-workers, peers and on nature. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>As you might know iGEM is not only about working in a lab, pipetting in lab coats and doing cool stuff with bacteria. iGEM is also about asking yourself the good questions when it comes to the implications that our project could have on our peers and on our environment. The team decided that evaluating our project with a risk assessment was a good way of bringing into focus what could be the impacts of our project on co-workers, peers and on nature. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Overview: Our project consists mainly on engineering E.<del class="diffchange diffchange-inline">Coli </del>in order to transport nanoparticles filled with drug to a specific site of action where the particles would be digested by a release of gelatinase<del class="diffchange diffchange-inline">. Thus </del>allowing the drug to enter this particular site where <del class="diffchange diffchange-inline">it’s </del>action is required. An application of such a device could be the treatment of <del class="diffchange diffchange-inline">the </del>colon cancer. E.coli being already a part of our gut flora, we thought it could be a good chassis to begin the experiments with. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Overview: Our project consists mainly on engineering E.<ins class="diffchange diffchange-inline">coli </ins>in order to transport nanoparticles filled with drug to a specific site of action where the particles would be digested by a release of gelatinase<ins class="diffchange diffchange-inline">, thus </ins>allowing the drug to enter this particular site where <ins class="diffchange diffchange-inline">its </ins>action is required. An application of such a device could be the treatment of colon cancer. E.coli being already a part of our gut flora, we thought it could be a good chassis to begin the experiments with. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:Team-EPF-Lausanne-gallery22.jpeg|thumb|center|500px|Figure 1: safety in our labs]] </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:Team-EPF-Lausanne-gallery22.jpeg|thumb|center|500px|Figure 1: safety in our labs]] </div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''a. Risks to the safety and health of team members or others working in the lab?'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''a. Risks to the safety and health of team members or others working in the lab?'''</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The biological materials we’re working with in the lab are especially thought to be as safe as possible for all the team and co-workers. Indeed our plan since the beginning of the iGEM project is to build a project<del class="diffchange diffchange-inline">, </del>which couldn’t raise any particular safety issues for us the students but also for our co-workers in the lab. We are particularly interested in designing a project that relies on simple ideas but which is still innovative, original and challenging. Besides our team followed some specific biosafety lectures given by Stéphane Karlen our school safety coordinator. We were particularly careful on wearing permanently our lab coats and gloves in the lab. Indeed working with RG1 biological material doesn’t mean that no precautions should be taken. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The biological materials we’re working with in the lab are especially thought to be as safe as possible for all the team and co-workers. Indeed<ins class="diffchange diffchange-inline">, </ins>our plan since the beginning of the iGEM project is to build a project which couldn’t raise any particular safety issues for us<ins class="diffchange diffchange-inline">, </ins>the students<ins class="diffchange diffchange-inline">, </ins>but also for our co-workers in the lab. We are particularly interested in designing a project that relies on simple ideas but which is still innovative, original and challenging. Besides our team followed some specific biosafety lectures given by Stéphane Karlen our school safety coordinator. We were particularly careful on wearing permanently our lab coats and gloves in the lab. Indeed working with RG1 biological material doesn’t mean that no precautions should be taken. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''b. Risks to the safety and health of the general public, if released by design or by accident?'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''b. Risks to the safety and health of the general public, if released by design or by accident?'''</div></td></tr>
</table>Leabernierhttp://2013.igem.org/wiki/index.php?title=Team:EPF_Lausanne/Safety&diff=303173&oldid=prevLeabernier: /* Risk assessment and safety: TAXI.COLI: smart drug delivery */2013-10-04T20:47:32Z<p><span class="autocomment">Risk assessment and safety: TAXI.COLI: smart drug delivery</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Overview: Our project consists mainly on engineering E.Coli in order to transport nanoparticles filled with drug to a specific site of action where the particles would be digested by a release of gelatinase. Thus allowing the drug to enter this particular site where it’s action is required. An application of such a device could be the treatment of the colon cancer. E.coli being already a part of our gut flora, we thought it could be a good chassis to begin the experiments with. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Overview: Our project consists mainly on engineering E.Coli in order to transport nanoparticles filled with drug to a specific site of action where the particles would be digested by a release of gelatinase. Thus allowing the drug to enter this particular site where it’s action is required. An application of such a device could be the treatment of the colon cancer. E.coli being already a part of our gut flora, we thought it could be a good chassis to begin the experiments with. </div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:Team-EPF-Lausanne-gallery22.jpeg|thumb|center|500px|<del class="diffchange diffchange-inline">Figure1</del>: safety in our labs]] </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:Team-EPF-Lausanne-gallery22.jpeg|thumb|center|500px|<ins class="diffchange diffchange-inline">Figure 1</ins>: safety in our labs]] </div></td></tr>
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</table>Leabernierhttp://2013.igem.org/wiki/index.php?title=Team:EPF_Lausanne/Safety&diff=300579&oldid=prevCbroenni at 19:10, 4 October 20132013-10-04T19:10:49Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''e. Risk Group of our chassis organism.'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''e. Risk Group of our chassis organism.'''</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><<del class="diffchange diffchange-inline">b</del>></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><<ins class="diffchange diffchange-inline">br</ins>></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>RG1</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>RG1</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>For more information and contact about biosafety and our project in EPFL: </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">''</ins>For more information and contact about biosafety and our project in EPFL: </div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Stéphane.Karlen@epfl.ch</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Stéphane.Karlen@epfl.ch</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Charlotte.Broennimann@epfl.ch</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Charlotte.Broennimann@epfl.ch</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:EPF-Lausanne-safe1.jpeg|thumb|center|500px|Figure1: safety in our labs]] </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:EPF-Lausanne-safe1.jpeg|thumb|center|500px|Figure1: safety in our labs]] </div></td></tr>
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</table>Cbroennihttp://2013.igem.org/wiki/index.php?title=Team:EPF_Lausanne/Safety&diff=300506&oldid=prevCbroenni at 19:08, 4 October 20132013-10-04T19:08:26Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:EPF-Lausanne-safe1.jpeg|thumb|center|500px|Figure1: safety in our labs]] </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:EPF-Lausanne-safe1.jpeg|thumb|center|500px|Figure1: safety in our labs]] </div></td></tr>
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</table>Cbroennihttp://2013.igem.org/wiki/index.php?title=Team:EPF_Lausanne/Safety&diff=300451&oldid=prevCbroenni at 19:06, 4 October 20132013-10-04T19:06:24Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The main problem that exists regarding the environment is resistance to antibiotics. Bacterial conjugation is the horizontal transfer of genetic material by direct cell-to-cell contact or by a bridge-like connection between two cells. This could happen when engineered bacteria containing plasmids resistant to antibiotics are released in the environment thus exchanging their genetic material with wild-type bacteria. Therefore providing antibiotic resistance to bacteria that originally are not resistant. A second potential risk is that the quantity of bacteria released in the environment may disrupt the environmental balance. However we are working with really small quantities of bacteria so the second potential risk is not likely to happen. Still for the antibiotic resistance some precautions are necessary to be undertaken. For example, our entire waste is first inactivated and then burned to prevent any release in the environment by our own waste treatment company in our university. All our used material is autoclaved and sterilized. Furthermore we are working with E.coli DH5alpha (K12), which is a lab strain that wouldn’t survive in the environment.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The main problem that exists regarding the environment is resistance to antibiotics. Bacterial conjugation is the horizontal transfer of genetic material by direct cell-to-cell contact or by a bridge-like connection between two cells. This could happen when engineered bacteria containing plasmids resistant to antibiotics are released in the environment thus exchanging their genetic material with wild-type bacteria. Therefore providing antibiotic resistance to bacteria that originally are not resistant. A second potential risk is that the quantity of bacteria released in the environment may disrupt the environmental balance. However we are working with really small quantities of bacteria so the second potential risk is not likely to happen. Still for the antibiotic resistance some precautions are necessary to be undertaken. For example, our entire waste is first inactivated and then burned to prevent any release in the environment by our own waste treatment company in our university. All our used material is autoclaved and sterilized. Furthermore we are working with E.coli DH5alpha (K12), which is a lab strain that wouldn’t survive in the environment.</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''d. Risks to security through malicious misuse by individuals, groups, or countries?'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''d. Risks to security through malicious misuse by individuals, groups, or countries?'''</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Our labs are under constant control thus preventing any misbehavior or intend to cause arm. Cameras, security agents and co-workers are on constant vigilance. Lab access is restricted at night and after working hours. Our main concern when designing our iGEM project was safety. We wanted to stick generally to RG1 organisms showing that great things can be accomplished also being respectful of the environment and people. Even in malicious hands our project couldn’t cause arm or cause a risk to the individuals, groups, countries or environment because it is designed to be safe since the beginning of its creation.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Our labs are under constant control thus preventing any misbehavior or intend to cause arm. Cameras, security agents and co-workers are on constant vigilance. Lab access is restricted at night and after working hours. Our main concern when designing our iGEM project was safety. We wanted to stick generally to RG1 organisms showing that great things can be accomplished also being respectful of the environment and people. Even in malicious hands our project couldn’t cause arm or cause a risk to the individuals, groups, countries or environment because it is designed to be safe since the beginning of its creation.</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''2: If your project moved from a small-scale lab study to become widely used as a commercial/industrial product, what new risks might arise? (Consider the different categories of risks that are listed in parts a-d of the previous question). Also, what risks might arise if the knowledge you generate or the methods you develop became widely available?'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''2: If your project moved from a small-scale lab study to become widely used as a commercial/industrial product, what new risks might arise? (Consider the different categories of risks that are listed in parts a-d of the previous question). Also, what risks might arise if the knowledge you generate or the methods you develop became widely available?'''</div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">'''4: What safety training have you received?''' </ins></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">All students at EPFL received specific lectures on biosafety and proper behavior in the lab. All members of the iGEM team followed these lectures and filled the safety form properly. </ins></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">All students at EPFL received specific lectures on biosafety and proper behavior in the lab. All members of the iGEM team followed these lectures and filled the safety form properly. </del></div></td><td colspan="2"> </td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''5: Under what biosafety provisions will / do you work?'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''5: Under what biosafety provisions will / do you work?'''</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>http://www.admin.ch/opc/fr/classified-compilation/19994946/index.html</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>http://www.admin.ch/opc/fr/classified-compilation/19994946/index.html</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''d. Biosafety Level rating of our lab according to WHO Biosafety Manual'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''d. Biosafety Level rating of our lab according to WHO Biosafety Manual'''</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BLS 1</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>BLS 1</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''e. Risk Group of our chassis organism.'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''e. Risk Group of our chassis organism.'''</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>RG1</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>RG1</div></td></tr>
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</table>Cbroennihttp://2013.igem.org/wiki/index.php?title=Team:EPF_Lausanne/Safety&diff=300398&oldid=prevCbroenni at 19:03, 4 October 20132013-10-04T19:03:59Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Overview: Our project consists mainly on engineering E.Coli in order to transport nanoparticles filled with drug to a specific site of action where the particles would be digested by a release of gelatinase. Thus allowing the drug to enter this particular site where it’s action is required. An application of such a device could be the treatment of the colon cancer. E.coli being already a part of our gut flora, we thought it could be a good chassis to begin the experiments with. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Overview: Our project consists mainly on engineering E.Coli in order to transport nanoparticles filled with drug to a specific site of action where the particles would be digested by a release of gelatinase. Thus allowing the drug to enter this particular site where it’s action is required. An application of such a device could be the treatment of the colon cancer. E.coli being already a part of our gut flora, we thought it could be a good chassis to begin the experiments with. </div></td></tr>
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</table>Cbroennihttp://2013.igem.org/wiki/index.php?title=Team:EPF_Lausanne/Safety&diff=300295&oldid=prevCbroenni at 18:59, 4 October 20132013-10-04T18:59:08Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Overview: Our project consists mainly on engineering E.Coli in order to transport nanoparticles filled with drug to a specific site of action where the particles would be digested by a release of gelatinase. Thus allowing the drug to enter this particular site where it’s action is required. An application of such a device could be the treatment of the colon cancer. E.coli being already a part of our gut flora, we thought it could be a good chassis to begin the experiments with. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Overview: Our project consists mainly on engineering E.Coli in order to transport nanoparticles filled with drug to a specific site of action where the particles would be digested by a release of gelatinase. Thus allowing the drug to enter this particular site where it’s action is required. An application of such a device could be the treatment of the colon cancer. E.coli being already a part of our gut flora, we thought it could be a good chassis to begin the experiments with. </div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''1: Do the biological materials used in your lab work pose any of the following risks? Please describe'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''1: Do the biological materials used in your lab work pose any of the following risks? Please describe'''</div></td></tr>
</table>Cbroenni