Team:Evry/Primers
From 2013.igem.org
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<table width="100%" cellpadding="10" cellspacing="0" align='center' border="1"> | <table width="100%" cellpadding="10" cellspacing="0" align='center' border="1"> | ||
- | <tr> | + | <tr> |
- | <th align="center"> | + | <th align="center"> |
- | <b>CODE</b> | + | <b>CODE</b> |
- | </th> | + | </th> |
- | <th class="colonne2" align="center" id="col2"> | + | <th class="colonne2" align="center" id="col2"> |
- | <div style="width: 200px;"><b>NAME</b></div> | + | <div style="width: 200px;"><b>NAME</b></div> |
- | </th> | + | </th> |
- | <th align="center"> | + | <th align="center"> |
- | <b>LENGTH</b> | + | <b>LENGTH</b> |
- | </th> | + | </th> |
- | <th align="center"> | + | <th align="center"> |
- | <b>ANNEALING<br/>TEMPERATURE</b> | + | <b>ANNEALING<br/>TEMPERATURE</b> |
- | </th> | + | </th> |
- | <th align="center" width="200px"> | + | <th align="center" width="200px"> |
- | <div style="width:"200px";"<b>SEQUENCE </b></div> | + | <div style="width:"200px";"<b>SEQUENCE </b></div> |
- | </th> | + | </th> |
- | </tr> | + | </tr> |
- | <tr> | + | <tr> |
- | <td align="center"> | + | <td align="center"> |
- | <p> | + | <p> |
- | P001 | + | P001 |
- | </p> | + | </p> |
- | </td> | + | </td> |
- | <td align="center"> | + | <td align="center"> |
- | <p> | + | <p> |
- | Primer Fur<br/>(Forward - GoldenBrick sequence) | + | Primer Fur<br/>(Forward - GoldenBrick sequence) |
- | </p> | + | </p> |
- | </td> | + | </td> |
- | <td align="center"> | + | <td align="center"> |
- | <p> | + | <p> |
- | 41 nt | + | 41 nt |
- | </p> | + | </p> |
- | </td> | + | </td> |
- | <td align="center"> | + | <td align="center"> |
- | <p> | + | <p> |
- | 74°C | + | 74°C |
- | </p> | + | </p> |
- | </td> | + | </td> |
- | <td> | + | <td> |
- | <p> | + | <p> |
- | CGTGAATTCGGTCTCTCTAGATGACTGATAACAATACCGCC | + | CGTGAATTCGGTCTCTCTAGATGACTGATAACAATACCGCC |
- | </p> | + | </p> |
- | </td> | + | </td> |
- | </tr> | + | </tr> |
- | <tr> | + | <tr> |
- | <td align="center"> | + | <td align="center"> |
- | <p> | + | <p> |
- | P002 | + | P002 |
- | </p> | + | </p> |
- | </td> | + | </td> |
- | <td align="center"> | + | <td align="center"> |
- | <p> | + | <p> |
- | Primer Fur<br/>(Reverse - GoldenBrick sequence) | + | Primer Fur<br/>(Reverse - GoldenBrick sequence) |
- | </p> | + | </p> |
- | </td> | + | </td> |
- | <td align="center"> | + | <td align="center"> |
- | <p> | + | <p> |
- | 48 nt | + | 48 nt |
- | </p> | + | </p> |
- | </td> | + | </td> |
- | <td align="center"> | + | <td align="center"> |
- | <p> | + | <p> |
- | 77°C | + | 77°C |
- | </p> | + | </p> |
- | </td> | + | </td> |
- | <td> | + | <td> |
- | <p> | + | <p> |
- | GGACTGCAGACTAGTGGTCTCTGTCTTCTTATTATTTGCCTTCGTGCG | + | GGACTGCAGACTAGTGGTCTCTGTCTTCTTATTATTTGCCTTCGTGCG |
- | </p> | + | </p> |
- | </td> | + | </td> |
- | </tr> | + | </tr> |
- | <tr> | + | <tr> |
- | <td align="center"> | + | <td align="center"> |
- | <p> | + | <p> |
- | P005 | + | P005 |
- | </p> | + | </p> |
- | </td> | + | </td> |
- | <td align="center"> | + | <td align="center"> |
- | <p> | + | <p> |
- | RBS B0034<br/>(Forward - GoldenBrick sequence) | + | RBS B0034<br/>(Forward - GoldenBrick sequence) |
- | </p> | + | </p> |
- | </td> | + | </td> |
- | <td align="center"> | + | <td align="center"> |
- | <p> | + | <p> |
- | 47 nt | + | 47 nt |
- | </p> | + | </p> |
- | </td> | + | </td> |
- | <td align="center"> | + | <td align="center"> |
- | <p> | + | <p> |
- | 75°C | + | 75°C |
- | </p> | + | </p> |
- | </td> | + | </td> |
- | <td> | + | <td> |
- | <p> | + | <p> |
- | GAATTCGGTCTCTTGCTAAAGAGGAGAAATACTAGTGAGACCCTGCA | + | GAATTCGGTCTCTTGCTAAAGAGGAGAAATACTAGTGAGACCCTGCA |
- | </p> | + | </p> |
- | </td> | + | </td> |
- | </tr> | + | </tr> |
- | <tr> | + | <tr> |
- | <td align="center"> | + | <td align="center"> |
- | <p> | + | <p> |
- | P006 | + | P006 |
- | </p> | + | </p> |
- | </td> | + | </td> |
- | <td align="center"> | + | <td align="center"> |
- | <p> | + | <p> |
- | RBS B0034<br/>(Reverse - GoldenBrick sequence) | + | RBS B0034<br/>(Reverse - GoldenBrick sequence) |
- | </p> | + | </p> |
- | </td> | + | </td> |
- | <td align="center"> | + | <td align="center"> |
- | <p> | + | <p> |
- | 47 nt | + | 47 nt |
- | </p> | + | </p> |
- | </td> | + | </td> |
- | <td align="center"> | + | <td align="center"> |
- | <p> | + | <p> |
- | 75°C | + | 75°C |
- | </p> | + | </p> |
- | </td> | + | </td> |
- | <td> | + | <td> |
- | <p> | + | <p> |
- | TGCAGGGTCTCACTAGTATTTCTCCTCTTTAGCAAGAGACCGAATTC | + | TGCAGGGTCTCACTAGTATTTCTCCTCTTTAGCAAGAGACCGAATTC |
- | </p> | + | </p> |
- | </td> | + | </td> |
- | </tr> | + | </tr> |
- | <tr> | + | <tr> |
- | <td align="center"> | + | <td align="center"> |
- | <p> | + | <p> |
- | P007 | + | P007 |
- | </p> | + | </p> |
- | </td> | + | </td> |
- | <td align="center"> | + | <td align="center"> |
- | <p> | + | <p> |
- | aceB Natural Fur promoter<br/>(Forward - GoldenBrick sequence) | + | aceB Natural Fur promoter<br/>(Forward - GoldenBrick sequence) |
- | </p> | + | </p> |
- | </td> | + | </td> |
- | <td align="center"> | + | <td align="center"> |
- | <p> | + | <p> |
- | 42 nt | + | 42 nt |
- | </p> | + | </p> |
- | </td> | + | </td> |
- | <td align="center"> | + | <td align="center"> |
- | <p> | + | <p> |
- | 76°C | + | 76°C |
- | </p> | + | </p> |
- | </td> | + | </td> |
- | <td> | + | <td> |
- | <p> | + | <p> |
- | GAATTCGGTCTCAGCCTCTAGAGATCTACGGCACATGAATCC | + | GAATTCGGTCTCAGCCTCTAGAGATCTACGGCACATGAATCC |
- | </p> | + | </p> |
- | </td> | + | </td> |
- | </tr> | + | </tr> |
<tr> | <tr> |
Revision as of 12:37, 6 August 2013
Primers
CODE |
NAME
|
LENGTH |
ANNEALING TEMPERATURE |
SEQUENCE
|
---|---|---|---|---|
P001 |
Primer Fur |
41 nt |
74°C |
CGTGAATTCGGTCTCTCTAGATGACTGATAACAATACCGCC |
P002 |
Primer Fur |
48 nt |
77°C |
GGACTGCAGACTAGTGGTCTCTGTCTTCTTATTATTTGCCTTCGTGCG |
P005 |
RBS B0034 |
47 nt |
75°C |
GAATTCGGTCTCTTGCTAAAGAGGAGAAATACTAGTGAGACCCTGCA |
P006 |
RBS B0034 |
47 nt |
75°C |
TGCAGGGTCTCACTAGTATTTCTCCTCTTTAGCAAGAGACCGAATTC |
P007 |
aceB Natural Fur promoter |
42 nt |
76°C |
GAATTCGGTCTCAGCCTCTAGAGATCTACGGCACATGAATCC |
P008 |
ace B Natural Fur promoter |
32 nt |
78°C |
TGCAGGGTCTCACTAGTACGTGCAGCTCCTCG |
P009 |
Ent C Natural Fur promoter |
49 nt |
73°C |
GAATTCGGTCTCAGCCTCTAGAGAAATCAGCTTCCTGTTATTAATAAGG |
P010 |
Ent C Natural Fur promoter |
32 nt |
64°C |
ACTAGTAATCATCCTCCACAAAATGATAAAGG |
P011 |
Fec A Natural Fur promoter |
50 nt |
73°C |
GAATTCGGTCTCAGCCTCTAGAGGGAAAATAATTCTTATTTCGATTGTCC |
P012 |
Fec A Natural Fur promoter |
41 nt |
74°C |
CTGCAGGGTCTCACTAGTAACCTTCCCCATCATCATTTTTG |
P013 |
Fec C Natural Fur promoter |
42 nt |
77°C |
GAATTCGGTCTCAGCCTCTAGAGGAACAGCAATTCAACCTGC |
P014 |
Fec C Natural Fur promoter |
36 nt |
75°C |
CTGCAGGGTCTCACTAGTATTCACAACGGTAAGCGG |
P015 |
Andersen's promoter J23100 |
70 nt |
84°C |
GAATTCGGTCTCAGCCTCTAGAGTTGACGGCTAGCTCAGTCCTAGGTACAGTGCT |
P016 |
Andersen's promoter J23100 |
70 nt |
84°C |
CGGTCTCATCACGCTAGCACTGTACCTAGGACTGAGCTAGCCGTCAACTCTAGAG |
P017 |
Fur Binding Site N°1 |
45 nt |
72°C |
CGGTCTCTGTGAATTATTGATAACTATTTGGATGCTAGAGACCAC |
P018 |
Fur Binding Site N°1 |
45 nt |
72°C |
GTGGTCTCTAGCATCCAAATAGTTATCAATAATTCACAGAGACCG |
P019 |
Fur Binding Site N°2 |
47 nt |
75°C |
CGGTCTCTGTGAGATAACTATTTGCATTTGCAGATGCTAGAGACCAC |
P020 |
Fur Binding Site N°2 |
47 nt |
75°C |
GTGGTCTCTAGCATCTGCAAATGCAAATAGTTATCTCACAGAGACCG |
P021 |
Fep A Natural Fur promoter |
41 nt |
76°C |
GAATTCGGTCTCAGCCTCTAGAGTTCGTCATTCAGACGCTG |
P022 |
Fep A Natural Fur promoter |
42 nt |
73°C |
CTGCAGGGTCTCACTAGTATGTTTTATTCCTGCATTTTTGCC |
P023 |
Fes Natural Fur promoter |
40 nt |
77°C |
GAATTCGGTCTCAGCCTCTAGAGGCCACGAATTGCAACTG |
P024 |
Fes Natural Fur promoter |
37 nt |
75°C |
CTGCAGGGTCTCACTAGTATTCGTCATTCAGACGCTG |
P025 |
Sdh C Natural Fur promoter |
45 nt |
75°C |
GAATTCGGTCTCAGCCTCTAGAGATCACTGTTCAGGATAAAACCC |
P026 |
Sdh C Natural Fur promoter |
36 nt |
76°C |
CTGCAGGGTCTCACTAGTAGAATAACGCCCACATGC |
P027 |
ybi L Natural Fur promoter |
50 nt |
76°C |
GAATTCGGTCTCAGCCTCTAGAGCACCTAATTTGCCCATGATGATATCTC |
P028 |
ybi L Natural Fur promoter |
50 nt |
72°C |
CTGCAGGGTCTCACTAGTAGTGATGGCTTCTGATAAAGATAAATTTATAG |
P029 |
ync E Natural Fur promoter |
40 nt |
78°C |
GAATTCGGTCTCAGCCTCTAGAGACGGGGGAAAGGACAAG |
P030 |
ync E Natural Fur promoter |
39 nt |
75°C |
CTGCAGGGTCTCACTAGTAGACGACTCCCTTTGATGAAC |
P031 |
Fur Binding Site N°3 |
47 nt |
75°C |
CGGTCTCTGTGACAAATGCAAATAGTTATCCAGATGCTAGAGACCAC |
P032 |
Fur Binding Site N°3 |
47 nt |
75°C |
GTGGTCTCTAGCATCTGGATAACTATTTGCATTTGTCACAGAGACCG |
P033 |
Fur Binding Site N°4 |
47 nt |
72°C |
CGGTCTCTGTGACAAATAGTTATCAATAATCAGATGCTAGAGACCAC |
P034 |
Fur Binding Site N°4 |
47 nt |
72°C |
GTGGTCTCTAGCATCTGATTATTGATAACTATTTGTCACAGAGACCG |
P035 |
Fur Binding Site N°5 |
47 nt |
70°C |
CGGTCTCTGTGAGTAAATTAATATTATTTACAGATGCTAGAGACCAC |
P036 |
Fur Binding Site N°5 |
47 nt |
70°C |
GTGGTCTCTAGCATCTGTAAATAATATTAATTTACTCACAGAGACCG |
P037 |
Fur Binding Site N°6 |
47 nt |
74°C |
CGGTCTCTGTGAAATAATGCTTCTCATTTTCAGATGCTAGAGACCAC |
P038 |
Fur Binding Site N°6 |
47 nt |
74°C |
GTGGTCTCTAGCATCTGAAAATGAGAAGCATTATTTCACAGAGACCG |
P039 |
Fur Binding Site N°7 |
47 nt |
71°C |
CGGTCTCTGTGAATAAATGATAATCATTATCAGATGCTAGAGACCAC |
P040 |
Fur Binding Site N°7 |
47 nt |
71°C |
GTGGTCTCTAGCATCTGATAATGATTATCATTTATTCACAGAGACCG |
P041 |
Fur Binding Site N°8 |
47 nt |
73°C |
CGGTCTCTGTGAGAAAATAATTCTTATTTCCAGATGCTAGAGACCAC |
P042 |
Fur Binding Site N°8 |
47 nt |
73°C |
GTGGTCTCTAGCATCTGGAAATAAGAATTATTTTCTCACAGAGACCG |
P043 |
Fur Binding Site N°9 |
47 nt |
73°C |
CGGTCTCTGTGAAGCACTTATTATTATTTTCAGATGCTAGAGACCAC |
P044 |
Fur Binding Site N°9 |
47 nt |
73°C |
GTGGTCTCTAGCATCTGAAAATAATAATAAGTGCTTCACAGAGACCG |
P045 |
Fur Binding Site N°10 |
47 nt |
75°C |
CGGTCTCTGTGAGATAATTGTTATCGTTTGCAGATGCTAGAGACCAC |
P046 |
Fur Binding Site N°10 |
47 nt |
75°C |
GTGGTCTCTAGCATCTGCAAACGATAACAATTATCTCACAGAGACCG |
P047 |
Fur Binding Site N°11 |
47 nt |
74°C |
CGGTCTCTGTGAGATAATGCTTATCAAAATCAGATGCTAGAGACCAC |
P048 |
Fur Binding Site N°11 |
47 nt |
74°C |
GTGGTCTCTAGCATCTGATTTTGATAAGCATTATCTCACAGAGACCG |
P049 |
Fur Binding Site N°12 |
47 nt |
73°C |
CGGTCTCTGTGACAAAATTATTATCACTTTCAGATGCTAGAGACCAC |
P050 |
Fur Binding Site N°12 |
47 nt |
73°C |
GTGGTCTCTAGCATCTGAAAGTGATAATAATTTTGTCACAGAGACCG |
P051 |
Fur Binding Site N°13 |
47 nt |
75°C |
CGGTCTCTGTGAAATAATGATTACCATTCCCAGATGCTAGAGACCAC |
P052 |
Fur Binding Site N°13 |
47 nt |
75°C |
GTGGTCTCTAGCATCTGGGAATGGTAATCATTATTTCACAGAGACCG |
P053 |
Fur Binding Site N°14 |
47 nt |
73°C |
CGGTCTCTGTGAGAAATTGTTTTTGATTTTCAGATGCTAGAGACCAC |
P054 |
Fur Binding Site N°14 |
47 nt |
73°C |
GTGGTCTCTAGCATCTGAAAATCAAAAACAATTTCTCACAGAGACCG |
P055 |
Fur Binding Site N°15 |
47 nt |
73°C |
CGGTCTCTGTGAGTTAATTGTAATGATTTTCAGATGCTAGAGACCAC |
P056 |
Fur Binding Site N°15 |
47 nt |
73°C |
GTGGTCTCTAGCATCTGAAAATCATTACAATTAACTCACAGAGACCG |
P057 |
Andersen's promoter J23101 |
70 nt |
81°C |
GAATTCGGTCTCAGCCTCTAGAGTTTACAGCTAGCTCAGTCCTAGGTATTATGCT |
P058 |
Andersen's promoter J23101 |
70 nt |
81°C |
CGGTCTCATCACGCTAGCATAATACCTAGGACTGAGCTAGCTGTAAACTCTAGAG |
P059 |
Andersen's promoter J23102 |
70 nt |
83°C |
GAATTCGGTCTCAGCCTCTAGAGTTGACAGCTAGCTCAGTCCTAGGTACTGTGCT |
P060 |
Andersen's promoter J23102 |
70 nt |
83°C |
CGGTCTCATCACGCTAGCACAGTACCTAGGACTGAGCTAGCTGTCAACTCTAGAG |
P061 |
Andersen's promoter J23104 |
70 nt |
82°C |
GAATTCGGTCTCAGCCTCTAGAGTTGACAGCTAGCTCAGTCCTAGGTATTGTGCT |
P062 |
Andersen's promoter J23104 |
70 nt |
82°C |
CGGTCTCATCACGCTAGCACAATACCTAGGACTGAGCTAGCTGTCAACTCTAGAG |
P063 |
VR primer iGEM standard |
20 nt |
63°C |
ATTACCGCCTTTGAGTGAGC |
P064 |
VF2 primer iGEM standard |
20 nt |
64°C |
TGCCACCTGACGTCTAAGAA |
P065 |
Ent A Biosynthesis of Enterobactin Siderophore |
74 nt |
81°C |
GAATTCGGTCTCTGTTCGGACCCATCTAGGTCCAGAGATAAGGAGGACAAACATG |
P066 |
Ent A Biosynthesis of Enterobactin Siderophore |
45 nt |
81°C |
GGACTGCAGACTAGTGGTCTCTTGGATCTTATGCCCCCAGCGTTG |
P067 |
Ent B Biosynthesis of Enterobactin Siderophore |
74 nt |
78°C |
GAATTCGGTCTCTGTTCACATACTAAACACATCAATCAAGGAGGTACTAGGATGG |
P068 |
Ent B Biosynthesis of Enterobactin Siderophore |
45 nt |
79°C |
GGACTGCAGACTAGTGGTCTCTTGGATCTTATTTCACCTCGCGGG |
P069 |
Ent C Biosynthesis of Enterobactin Siderophore |
68 nt |
80°C |
GAATTCGGTCTCTTCCAATACTAAGCAGTTACAAGGATAAGGAGGAGCAACATGG |
P070 |
Ent C Biosynthesis of Enterobactin Siderophore |
53 nt |
76°C |
GGACTGCAGACTAGTGGTCTCTGTTCTCTTAATGCAATCCAAAAACGTTCAAC |
P071 |
Ent D Biosynthesis of Enterobactin Siderophore |
70 nt |
83°C |
GAATTCGGTCTCTTCCACACCCCGAAGAACAGCAAAGGCGGACGAACAATGGTCG |
P072 |
Ent D Biosynthesis of Enterobactin Siderophore |
47 nt |
79°C |
GGACTGCAGACTAGTGGTCTCTGTTCTCTTAATCGTGTTGGCACAGC |
P073 |
Ent E Biosynthesis of Enterobactin Siderophore |
71 nt |
80°C |
GAATTCGGTCTCTGAACGAGCTAAAAGTAGCGCCCAATATAAGGAGGTCAAAATG |
P074 |
Ent E Biosynthesis of Enterobactin Siderophore |
43 nt |
81°C |
GGACTGCAGACTAGTGGTCTCTGTCTTCTCAGGCTGATGCGCG |
P075 |
Ent F Biosynthesis of Enterobactin Siderophore |
68 nt |
75°C |
GAATTCGGTCTCTGAACACATCTATATTTATCTTAAGGAGTACAATCATGAGCCA |
P076 |
Ent F Biosynthesis of Enterobactin Siderophore |
47 nt |
78°C |
GGACTGCAGACTAGTGGTCTCTGTCTTCTTACCTGTTTAGCGTTGCG |
P077 |
RBS sfGFP - Plasmid 3 |
65 nt |
76°C |
CGTGAATTCGGTCTCTGTTCTGAAAGAACTATATCTACAAAATCCATATGCGTAA |
P078 |
RBS sfGFP - Plasmid 3 |
58 nt |
75°C |
GGACTGCAGACTAGTGGTCTCTGTCTTCTTATTATTTGTACAGTTCATCCATACCATG |
P079 |
Promoteur Lac + Lac Operator |
93 nt |
82°C |
GAATTCGGTCTCAGCCTCTAGAGAATTGTGAGCGGATAACAATTGACATTGTGAG |
P080 |
Promoteur Lac + Lac Operator |
93 nt |
82°C |
GTGGTCTCTGAACTCTGTGCTCAGTATCTTGTTATCCGCTCACAATGTCAATTGT |
P081 |
RBS positive control - Plasmid 2 |
46 nt |
77°C |
CGGTCTCTGTGATGCTAAAGAGGAGAAATACTAGTGAGACCCTGCA |
P082 |
RBS positive control - Plasmid 2 |
46 nt |
77°C |
TGCAGGGTCTCACTAGTATTTCTCCTCTTTAGCATCACAGAGACCG |
P083 |
Lac I - Plasmid 1/2 |
40 nt |
72°C |
GAATTCGGTCTCTCTAGATGGTGAATGTGAAACCAGTAAC |
P084 |
Lac I - Plasmid 1/2 |
56 nt |
76°C |
GGACTGCAGACTAGTGGTCTCTGTCTTCTTATTAAGCTACTAAAGCGTAGTTTTCG |