P001

Primer Fur (Forward - GoldenBrick sequence)

--- bp

---°C

CGTGAATTCGGTCTCTCTAGATGACTGATAACAATACCGCC

P002

Primer Fur (Reverse - GoldenBrick sequence)

--- bp

---°C

GGACTGCAGACTAGTGGTCTCTGTCTTCTTATTATTTGCCTTCGTGCG

P005

RBS B0034 (Forward - GoldenBrick sequence)

--- bp

---°C

GAATTCGGTCTCTTGCTAAAGAGGAGAAATACTAGTGAGACCCTGCA

P006

RBS B0034 (Reverse - GoldenBrick sequence)

--- bp

---°C

TGCAGGGTCTCACTAGTATTTCTCCTCTTTAGCAAGAGACCGAATTC

P007

aceB Natural Fur promoter (Forward - GoldenBrick sequence)

--- bp

---°C

GAATTCGGTCTCAGCCTCTAGAGATCTACGGCACATGAATCC

P008

ace B Natural Fur promoter (Reverse - GoldenBrick sequence)

--- bp

---°C

TGCAGGGTCTCACTAGTACGTGCAGCTCCTCG

P009

Ent C Natural Fur promoter (Forward - GoldenBrick sequence)

--- bp

---°C

GAATTCGGTCTCAGCCTCTAGAGAAATCAGCTTCCTGTTATTAATAAGG

P010

Ent C Natural Fur promoter (Reverse - GoldenBrick sequence)

--- bp

---°C

ACTAGTAATCATCCTCCACAAAATGATAAAGG

P011

Fec A Natural Fur promoter (Forward - GoldenBrick sequence)

--- bp

---°C

GAATTCGGTCTCAGCCTCTAGAGGGAAAATAATTCTTATTTCGATTGTCC

P012

Fec A Natural Fur promoter (Reverse - GoldenBrick sequence)

--- bp

---°C

CTGCAGGGTCTCACTAGTAACCTTCCCCATCATCATTTTTG

P013

Fec C Natural Fur promoter (Forward - GoldenBrick sequence)

--- bp

---°C

GAATTCGGTCTCAGCCTCTAGAGGAACAGCAATTCAACCTGC

P014

Fec C Natural Fur promoter (Reverse - GoldenBrick sequence)

--- bp

---°C

CTGCAGGGTCTCACTAGTATTCACAACGGTAAGCGG

P015

Andersen's promoter J23100 (Forward - GoldenBrick sequence)

--- bp

---°C

GAATTCGGTCTCAGCCTCTAGAGTTGACGGCTAGCTCAGTCCTAGGTACAGTGCTAGCGTGATGAGACCG

P016

Andersen's promoter J23100 (Reverse - GoldenBrick sequence)

--- bp

---°C

CGGTCTCATCACGCTAGCACTGTACCTAGGACTGAGCTAGCCGTCAACTCTAGAGGCTGAGACCGAATTC

P017

Fur Binding Site N°1 (Foward - GoldenBrick sequence)

--- bp

---°C

CGGTCTCTGTGAATTATTGATAACTATTTGGATGCTAGAGACCAC

P018

Fur Binding Site N°1 (Reverse - GoldenBrick sequence)

--- bp

---°C

GTGGTCTCTAGCATCCAAATAGTTATCAATAATTCACAGAGACCG

P019

Fur Binding Site N°2 (Foward - GoldenBrick sequence)

--- bp

---°C

CGGTCTCTGTGAGATAACTATTTGCATTTGCAGATGCTAGAGACCAC

P020

Fur Binding Site N°2 (Reverse - GoldenBrick sequence)

--- bp

---°C

GTGGTCTCTAGCATCTGCAAATGCAAATAGTTATCTCACAGAGACCG

P021

Fep A Natural Fur promoter (Foward - GoldenBrick sequence)

--- bp

---°C

GAATTCGGTCTCAGCCTCTAGAGTTCGTCATTCAGACGCTG

P022

Fep A Natural Fur promoter (Reverse - GoldenBrick sequence)

--- bp

---°C

CTGCAGGGTCTCACTAGTATGTTTTATTCCTGCATTTTTGCC

P023

Fes Natural Fur promoter (Foward - GoldenBrick sequence)

--- bp

---°C

GAATTCGGTCTCAGCCTCTAGAGGCCACGAATTGCAACTG

P024

Fes Natural Fur promoter (Reverse - GoldenBrick sequence)

--- bp

---°C

CTGCAGGGTCTCACTAGTATTCGTCATTCAGACGCTG

P025

Sdh C Natural Fur promoter (Foward - GoldenBrick sequence)

--- bp

---°C

GAATTCGGTCTCAGCCTCTAGAGATCACTGTTCAGGATAAAACCC

P026

Sdh C Natural Fur promoter (Reverse - GoldenBrick sequence)

--- bp

---°C

CTGCAGGGTCTCACTAGTAGAATAACGCCCACATGC

P027

ybi L Natural Fur promoter (Forward - GoldenBrick sequence)

--- bp

---°C

GAATTCGGTCTCAGCCTCTAGAGCACCTAATTTGCCCATGATGATATCTC

P028

ybi L Natural Fur promoter (Reverse - GoldenBrick sequence)

--- bp

---°C

CTGCAGGGTCTCACTAGTAGTGATGGCTTCTGATAAAGATAAATTTATAG

P029

ync E Natural Fur promoter (Forward - GoldenBrick sequence)

--- bp

---°C

GAATTCGGTCTCAGCCTCTAGAGACGGGGGAAAGGACAAG

P030

ync E Natural Fur promoter (Reverse - GoldenBrick sequence)

--- bp

---°C

CTGCAGGGTCTCACTAGTAGACGACTCCCTTTGATGAAC

P031

Fur Binding Site N°3 (Forward - GoldenBrick sequence)

--- bp

---°C

CGGTCTCTGTGACAAATGCAAATAGTTATCCAGATGCTAGAGACCAC

P032

Fur Binding Site N°3 (Reverse - GoldenBrick sequence)

--- bp

---°C

GTGGTCTCTAGCATCTGGATAACTATTTGCATTTGTCACAGAGACCG

P033

Fur Binding Site N°4 (Forward - GoldenBrick sequence)

--- bp

---°C

CGGTCTCTGTGACAAATAGTTATCAATAATCAGATGCTAGAGACCAC

P034

Fur Binding Site N°4 (Reverse - GoldenBrick sequence)

--- bp

---°C

GTGGTCTCTAGCATCTGATTATTGATAACTATTTGTCACAGAGACCG

P035

Fur Binding Site N°5 (Forward - GoldenBrick sequence)

--- bp

---°C

CGGTCTCTGTGAGTAAATTAATATTATTTACAGATGCTAGAGACCAC

P036

Fur Binding Site N°5 (Reverse - GoldenBrick sequence)

--- bp

---°C

GTGGTCTCTAGCATCTGTAAATAATATTAATTTACTCACAGAGACCG

P037

Fur Binding Site N°6 (Forward - GoldenBrick sequence)

--- bp

---°C

CGGTCTCTGTGAAATAATGCTTCTCATTTTCAGATGCTAGAGACCAC

P038

Fur Binding Site N°6 (Reverse - GoldenBrick sequence)

--- bp

---°C

GTGGTCTCTAGCATCTGAAAATGAGAAGCATTATTTCACAGAGACCG

P039

Fur Binding Site N°7 (Forward - GoldenBrick sequence)

--- bp

---°C

CGGTCTCTGTGAATAAATGATAATCATTATCAGATGCTAGAGACCAC

P040

Fur Binding Site N°7 (Reverse - GoldenBrick sequence)

--- bp

---°C

GTGGTCTCTAGCATCTGATAATGATTATCATTTATTCACAGAGACCG

P041

Fur Binding Site N°8 (Forward - GoldenBrick sequence)

--- bp

---°C

CGGTCTCTGTGAGAAAATAATTCTTATTTCCAGATGCTAGAGACCAC

P042

Fur Binding Site N°8 (Reverse - GoldenBrick sequence)

--- bp

---°C

GTGGTCTCTAGCATCTGGAAATAAGAATTATTTTCTCACAGAGACCG

P043

Fur Binding Site N°9 (Forward - GoldenBrick sequence)

--- bp

---°C

CGGTCTCTGTGAAGCACTTATTATTATTTTCAGATGCTAGAGACCAC

P044

Fur Binding Site N°9 (Reverse - GoldenBrick sequence)

--- bp

---°C

GTGGTCTCTAGCATCTGAAAATAATAATAAGTGCTTCACAGAGACCG

P045

Fur Binding Site N°10 (Forward - GoldenBrick sequence)

--- bp

---°C

CGGTCTCTGTGAGATAATTGTTATCGTTTGCAGATGCTAGAGACCAC

P046

Fur Binding Site N°10 (Reverse - GoldenBrick sequence)

--- bp

---°C

GTGGTCTCTAGCATCTGCAAACGATAACAATTATCTCACAGAGACCG

P047

Fur Binding Site N°11 (Forward - GoldenBrick sequence)

--- bp

---°C

CGGTCTCTGTGAGATAATGCTTATCAAAATCAGATGCTAGAGACCAC

P048

Fur Binding Site N°11 (Reverse - GoldenBrick sequence)

--- bp

---°C

GTGGTCTCTAGCATCTGATTTTGATAAGCATTATCTCACAGAGACCG

P049

Fur Binding Site N°12 (Forward - GoldenBrick sequence)

--- bp

---°C

CGGTCTCTGTGACAAAATTATTATCACTTTCAGATGCTAGAGACCAC

P050

Fur Binding Site N°12 (Reverse - GoldenBrick sequence)

--- bp

---°C

GTGGTCTCTAGCATCTGAAAGTGATAATAATTTTGTCACAGAGACCG

P051

Fur Binding Site N°13 (Forward - GoldenBrick sequence)

--- bp

---°C

CGGTCTCTGTGAAATAATGATTACCATTCCCAGATGCTAGAGACCAC

P052

Fur Binding Site N°13 (Reverse - GoldenBrick sequence)

--- bp

---°C

GTGGTCTCTAGCATCTGGGAATGGTAATCATTATTTCACAGAGACCG

P053

Fur Binding Site N°14 (Forward - GoldenBrick sequence)

--- bp

---°C

CGGTCTCTGTGAGAAATTGTTTTTGATTTTCAGATGCTAGAGACCAC

P054

Fur Binding Site N°14 (Reverse - GoldenBrick sequence)

--- bp

---°C

GTGGTCTCTAGCATCTGAAAATCAAAAACAATTTCTCACAGAGACCG

P055

Fur Binding Site N°15 (Forward - GoldenBrick sequence)

--- bp

---°C

CGGTCTCTGTGAGTTAATTGTAATGATTTTCAGATGCTAGAGACCAC

P056

Fur Binding Site N°15 (Reverse - GoldenBrick sequence)

--- bp

---°C

GTGGTCTCTAGCATCTGAAAATCATTACAATTAACTCACAGAGACCG

P057

Andersen's promoter J23101 (Forward - GoldenBrick sequence)

--- bp

---°C

GAATTCGGTCTCAGCCTCTAGAGTTTACAGCTAGCTCAGTCCTAGGTATTATGCTAGCGTGATGAGACCG

P058

Andersen's promoter J23101 (Reverse - GoldenBrick sequence)

--- bp

---°C

CGGTCTCATCACGCTAGCATAATACCTAGGACTGAGCTAGCTGTAAACTCTAGAGGCTGAGACCGAATTC

P059

Andersen's promoter J23102 (Forward - GoldenBrick sequence)

--- bp

---°C

GAATTCGGTCTCAGCCTCTAGAGTTGACAGCTAGCTCAGTCCTAGGTACTGTGCTAGCGTGATGAGACCG

P060

Andersen's promoter J23102 (Reverse - GoldenBrick sequence)

--- bp

---°C

CGGTCTCATCACGCTAGCACAGTACCTAGGACTGAGCTAGCTGTCAACTCTAGAGGCTGAGACCGAATTC

P061

Andersen's promoter J23104 (Forward - GoldenBrick sequence)

--- bp

---°C

GAATTCGGTCTCAGCCTCTAGAGTTGACAGCTAGCTCAGTCCTAGGTATTGTGCTAGCGTGATGAGACCG

P062

Andersen's promoter J23104 (Reverse - GoldenBrick sequence)

--- bp

---°C

CGGTCTCATCACGCTAGCACAATACCTAGGACTGAGCTAGCTGTCAACTCTAGAGGCTGAGACCGAATTC

P063

VR primer iGEM standard

--- bp

---°C

ATTACCGCCTTTGAGTGAGC

P064

VF2 primer iGEM standard

--- bp

---°C

TGCCACCTGACGTCTAAGAA

P065

Ent A Biosynthesis of Enterobactin Siderophore (Forward - GoldenBrick sequence)

--- bp

---°C

GAATTCGGTCTCTGTTCGGACCCATCTAGGTCCAGAGATAAGGAGGACAAACATGGATTTCAGCGGTAAAAATG

P066

Ent A Biosynthesis of Enterobactin Siderophore (Reverse - GoldenBrick sequence)

--- bp

---°C

GGACTGCAGACTAGTGGTCTCTTGGATCTTATGCCCCCAGCGTTG

P067

Ent B Biosynthesis of Enterobactin Siderophore (Forward - GoldenBrick sequence)

--- bp

---°C

GAATTCGGTCTCTGTTCACATACTAAACACATCAATCAAGGAGGTACTAGGATGGCTATTCCAAAATTACAGGC

P068

Ent B Biosynthesis of Enterobactin Siderophore (Reverse - GoldenBrick sequence)

--- bp

---°C

GGACTGCAGACTAGTGGTCTCTTGGATCTTATTTCACCTCGCGGG

P069

Ent C Biosynthesis of Enterobactin Siderophore (Forward - GoldenBrick sequence)

--- bp

---°C

GAATTCGGTCTCTTCCAATACTAAGCAGTTACAAGGATAAGGAGGAGCAACATGGATACGTCACTGGC

P070

Ent C Biosynthesis of Enterobactin Siderophore (Reverse - GoldenBrick sequence)

--- bp

---°C

GGACTGCAGACTAGTGGTCTCTGTTCTCTTAATGCAATCCAAAAACGTTCAAC

P071

Ent D Biosynthesis of Enterobactin Siderophore (Forward - GoldenBrick sequence)

--- bp

---°C

GAATTCGGTCTCTTCCACACCCCGAAGAACAGCAAAGGCGGACGAACAATGGTCGATATGAAAACTACGC

P072

Ent D Biosynthesis of Enterobactin Siderophore (Reverse - GoldenBrick sequence)

--- bp

---°C

GGACTGCAGACTAGTGGTCTCTGTTCTCTTAATCGTGTTGGCACAGC

P073

Ent E Biosynthesis of Enterobactin Siderophore (Forward - GoldenBrick sequence)

--- bp

---°C

GAATTCGGTCTCTGAACGAGCTAAAAGTAGCGCCCAATATAAGGAGGTCAAAATGAGCATTCCATTCACCC

P074

Ent E Biosynthesis of Enterobactin Siderophore (Reverse - GoldenBrick sequence)

--- bp

---°C

GGACTGCAGACTAGTGGTCTCTGTCTTCTCAGGCTGATGCGCG

P075

Ent F Biosynthesis of Enterobactin Siderophore (Forward - GoldenBrick sequence)

--- bp

---°C

GAATTCGGTCTCTGAACACATCTATATTTATCTTAAGGAGTACAATCATGAGCCAGCATTTACCTTTG

P076

Ent F Biosynthesis of Enterobactin Siderophore (Reverse - GoldenBrick sequence)

--- bp

---°C

GGACTGCAGACTAGTGGTCTCTGTCTTCTTACCTGTTTAGCGTTGCG

P077

RBS sfGFP - Plasmid 3 (Forward - GoldenBrick sequence)

--- bp

---°C

CGTGAATTCGGTCTCTGTTCTGAAAGAACTATATCTACAAAATCCATATGCGTAAAGGCGAAGAG

P078

RBS sfGFP - Plasmid 3 (Reverse - GoldenBrick sequence)

--- bp

---°C

GGACTGCAGACTAGTGGTCTCTGTCTTCTTATTATTTGTACAGTTCATCCATACCATG

P079

Promoteur Lac + Lac Operator (Forward - GoldenBrick sequence)

--- bp

---°C

GAATTCGGTCTCAGCCTCTAGAGAATTGTGAGCGGATAACAATTGACATTGTGAGCGGATAACAAGATACTGAGCACAGAGTTCAGAGACCAC

P080

Promoteur Lac + Lac Operator (Reverse - GoldenBrick sequence)

--- bp

---°C

GTGGTCTCTGAACTCTGTGCTCAGTATCTTGTTATCCGCTCACAATGTCAATTGTTATCCGCTCACAATTCTCTAGAGGCTGAGACCGAATTC

P081

RBS positive control - Plasmid 2 (Forward - GoldenBrick sequence)

--- bp

---°C

CGGTCTCTGTGATGCTAAAGAGGAGAAATACTAGTGAGACCCTGCA

P082

RBS positive control - Plasmid 2 (Reverse - GoldenBrick sequence)

--- bp

---°C

TGCAGGGTCTCACTAGTATTTCTCCTCTTTAGCATCACAGAGACCG

P083

Lac I - Plasmid 1/2 (Forward - GoldenBrick sequence)

--- bp

---°C

GAATTCGGTCTCTCTAGATGGTGAATGTGAAACCAGTAAC

P084

Lac I - Plasmid 1/2 (Reverse - GoldenBrick sequence)

--- bp

---°C

GGACTGCAGACTAGTGGTCTCTGTCTTCTTATTAAGCTACTAAAGCGTAGTTTTCG