http://2013.igem.org/wiki/index.php?title=Team:GeorgiaTech/Agar_Plates_and_Media_Protocol&feed=atom&action=historyTeam:GeorgiaTech/Agar Plates and Media Protocol - Revision history2024-03-29T11:23:54ZRevision history for this page on the wikiMediaWiki 1.16.5http://2013.igem.org/wiki/index.php?title=Team:GeorgiaTech/Agar_Plates_and_Media_Protocol&diff=219486&oldid=prevTilak94 at 20:14, 27 September 20132013-09-27T20:14:46Z<p></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 20:14, 27 September 2013</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Add (35 g/L of LB [[Agar]]) or (25 g/L [[LB Broth|LB broth]] and 15 g/L [[Agar]]) to a flask with distilled water</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Add (35 g/L of LB [[Agar]]) or (25 g/L [[LB Broth|LB broth]] and 15 g/L [[Agar]]) to a flask with distilled water</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*Dissolve and autoclave for <del class="diffchange diffchange-inline">30 mins (Basement lab [[Autoclave Machine|autoclave machine]]) or </del>1 hour <del class="diffchange diffchange-inline">( Barker lab [[Autoclave Machine|autoclave machine]])</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*Dissolve and autoclave for 1 hour <ins class="diffchange diffchange-inline">on fluid cycle</ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Antibiotics should be added at the correct concentration and volume when the [[agar]] has cooled to around 50°C or cool enough for hands to be touching the exterior of the flask.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Antibiotics should be added at the correct concentration and volume when the [[agar]] has cooled to around 50°C or cool enough for hands to be touching the exterior of the flask.</div></td></tr>
</table>Tilak94http://2013.igem.org/wiki/index.php?title=Team:GeorgiaTech/Agar_Plates_and_Media_Protocol&diff=218685&oldid=prevTilak94: Created page with "*Add (35 g/L of LB Agar) or (25 g/L LB broth and 15 g/L Agar) to a flask with distilled water *Dissolve and autoclave for 30 mins (Basement lab [[Autoclave ..."2013-09-27T19:54:13Z<p>Created page with "*Add (35 g/L of LB <a href="/wiki/index.php?title=Agar&action=edit&redlink=1" class="new" title="Agar (page does not exist)">Agar</a>) or (25 g/L <a href="/wiki/index.php?title=LB_Broth&action=edit&redlink=1" class="new" title="LB Broth (page does not exist)">LB broth</a> and 15 g/L <a href="/wiki/index.php?title=Agar&action=edit&redlink=1" class="new" title="Agar (page does not exist)">Agar</a>) to a flask with distilled water *Dissolve and autoclave for 30 mins (Basement lab [[Autoclave ..."</p>
<p><b>New page</b></p><div>*Add (35 g/L of LB [[Agar]]) or (25 g/L [[LB Broth|LB broth]] and 15 g/L [[Agar]]) to a flask with distilled water<br />
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*Dissolve and autoclave for 30 mins (Basement lab [[Autoclave Machine|autoclave machine]]) or 1 hour ( Barker lab [[Autoclave Machine|autoclave machine]])<br />
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*Antibiotics should be added at the correct concentration and volume when the [[agar]] has cooled to around 50°C or cool enough for hands to be touching the exterior of the flask.<br />
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*'''Concentrations of antibiotics:''' <br />
**Chloramphenicol 34 µg/µL - Blue <br />
**Kanamycin 50 µg/µL - Green<br />
**Ampicillin 100 µg/µL - Red<br />
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*For every mL of LB [[agar]] solution, add 1 µL of antibiotics. <br />
**Since I was using 300 mL of water, I added .3 mL of kanamycin<br />
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*The [[plates]] are poured and cooled, then marked with a marker to signify the antibiotic.<br />
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*[[Agar]] stabs are from the same protocol, just poured into tubes and capped.<br />
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*Media solution is made from [[LB Broth|LB broth]] (25 g/L), autoclaved, and antibiotics added to it. <br />
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*From the toothpick used to create an [[agar]] stab, the toothpick is placed into the media solution to start the culture.</div>Tilak94