Team:Glendale CC AZ/Protocols/Ligation

From 2013.igem.org

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== Ligation Protocol ==
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Protocol from: http://parts.igem.org/Help:Protocols/Ligation
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== Materials ==
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  -digested plasmid backbone
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  -equimolar amount of EcoRI-HF SpeI digested fragment
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  -equimolar amount of XbaI PstI digested fragment
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  -T4 DNA ligase buffer
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  -T4 DNA ligase
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  -dH2O
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== Procedure ==

Revision as of 19:58, 11 August 2013


Ligation Protocol

Protocol from: http://parts.igem.org/Help:Protocols/Ligation

Materials 

  -digested plasmid backbone
  -equimolar amount of EcoRI-HF SpeI digested fragment
  -equimolar amount of XbaI PstI digested fragment
  -T4 DNA ligase buffer
  -T4 DNA ligase
  -dH2O

Procedure

Retrieved from "http://2013.igem.org/Team:Glendale_CC_AZ/Protocols/Ligation"