Team:Glendale CC AZ/Protocols/Ligation

From 2013.igem.org

Revision as of 07:55, 18 August 2013 by EstherJ (Talk | contribs)


Ligation Protocol

Protocol from: http://parts.igem.org/Help:Protocols/Ligation

Materials 

  -digested plasmid backbone
  -equimolar amount of EcoRI-HF SpeI digested fragment
  -equimolar amount of XbaI PstI digested fragment
  -T4 DNA ligase buffer
  -T4 DNA ligase
  -dH2O

Procedure

Retrieved from "http://2013.igem.org/Team:Glendale_CC_AZ/Protocols/Ligation"