Team:Glendale CC AZ/Protocols/PCR

From 2013.igem.org

(Difference between revisions)
(Materials)
Line 11: Line 11:
   -2µl Primer 1
   -2µl Primer 1
   -2µl Primer 2
   -2µl Primer 2
-
   -1.5µl/ 3µl MgCl2
+
   -1.5µl MgCl2
   -5µl PCR buffer
   -5µl PCR buffer
   -0.5µl Taq Polymerase
   -0.5µl Taq Polymerase
== Procedure ==
== Procedure ==

Revision as of 22:08, 25 August 2013


Polymerase Chain Reaction Protocol

Materials

  -35.5µl dH2O
  -2µl dNTPs
  -2µl Primer 1
  -2µl Primer 2
  -1.5µl MgCl2
  -5µl PCR buffer
  -0.5µl Taq Polymerase

Procedure