Team:Glendale CC AZ/tests

From 2013.igem.org

(Difference between revisions)
Line 1: Line 1:
-
[[File:GCCmini.png|120px|left|alt text]]
 
-
[[File:Gel.png|120px|left|alt text]]
 
-
 
-
{|align="center"
 
-
|[[File:Gcclogo.gif|center|]]
 
-
|align="center"|
 
-
|}
 
-
 
-
<!-- ie9.js (fixes all Internet Explorer browsers older than ie9) -->
 
-
<!--[if lt IE 9]>
 
-
<script src="http://ie7-js.googlecode.com/svn/version/2.1(beta4)/IE9.js"></script>
 
-
<![endif]-->
 
-
 
-
 
-
 
<html>
<html>
-
 
<head>
<head>
-
<style>
+
<meta charset="UTF-8">
-
body
+
<title>Untitled Document</title>
-
{
+
<style type="text/css">* {
-
background:#ffffff url('img_tree.png') no-repeat right top;
+
  margin: 0;
-
margin-right:200px;
+
  padding: 0;
 +
  border: 0;
 +
  outline: none;
 +
  text-decoration: none;
 +
  list-style: none;
 +
  font-weight: 200;
 +
  -webkit-font-smoothing: antialiased;
 +
  line-height: 1em;
 +
  font-family: segoe ui, helvetica neue, helvetica, arial, sans-serif;
 +
  box-sizing: border-box;
 +
  -moz-box-sizing: border-box;
}
}
-
</style>
 
-
</head>
 
-
<html>
+
/*--Formatting to remove and alter wiki css defaults--*/
-
<head>
+
#contentSub, .firstHeading, #footer-box, #catlinks, #search-controls { display:none;}
-
<style>
+
#top-section {padding-top: 0px; border: none;}
 +
#globalWrapper, #content { width: 100%; background-color: #fff; border: none; margin: 0; padding: 0; position: relative;}
 +
html, body { height: 100%; background: #fff;}
 +
body {font: 10px normal Verdana, Arial, Helvetica, sans-serif;  color: #333; background: #f0f0f0;}
 +
#newcontainer { /*-- Footer specific edits http://ryanfait.com/resources/footer-stick-to-bottom-of-page/ --*/
 +
width:975px;
 +
margin-left: auto;
 +
margin-right: auto;
 +
position:relative;
 +
min-height: 100%;
 +
height: auto !important;
 +
height: 100%;
 +
border: 0px solid #000;
 +
margin: 0 auto -250px;
 +
color: #333;
 +
}
 +
/* Defaults */
 +
body {
 +
  font-size: 16px;
 +
  background: #e5e5e5;
 +
}
-
#contentSub, #search-controls, #footer-box, .firstHeading, #catlinks, #p-logo {
+
h1 {
-
    display:none;}
+
  font-size: 180%;
-
#top-section {
+
  color: #fff;
-
    border: none;
+
}
-
    height: 0px;}
+
-
#content {
+
-
    border: none;}
+
 +
h2 {
 +
  font-size: 160%;
 +
  color: #666;
 +
  padding-bottom: 15px;
 +
}
-
/* Removes "teams" from the menubar */
+
h3 {
-
#menubar > ul > li:last-child {
+
  font-size: 120%;
-
    display: none;}
+
  color: #ccc;
-
/* Resizes the menubar to fik the links (default is 400px) */
+
  padding-bottom: 15px;
-
#menubar {
+
}
-
    width: auto;}
+
 +
img {
 +
  display: block;
 +
width: 100%;
 +
}
-
body {
+
p, a, span, li {
-
    margin: 10px 0 0 0;
+
  font-size: 90%;
-
    padding: 0;}
+
  line-height: 25px;
-
#top-section {
+
  color: #666;
-
    width: 965px;
+
}
-
    height: 0;
+
-
    margin: 0 auto;
+
-
    padding: 0;
+
-
    border: none;}
+
-
#menubar {
+
-
    font-size: 65%;
+
-
    top: -14px;}
+
-
.left-menu:hover {
+
-
    background-color: transparent;}
+
-
#menubar li a {
+
-
    background-color: transparent;}
+
-
#menubar:hover {
+
-
    color: white;}
+
-
#menubar li a {
+
-
    color: transparent;}
+
-
#menubar:hover li a {
+
-
    color: white;}
+
 +
p a, li a, h1 a, p span {
 +
  color: inherit;
 +
  font-size: inherit;
 +
}
 +
p a {
 +
  color: #48C7C1;
 +
}
 +
p a:hover {
 +
  text-decoration: underline;
 +
}
 +
p.tags {
 +
  padding-top: 10px;
 +
}
 +
p.tags a {
 +
  padding: 0px 5px 0px 5px;
 +
  background: #222;
 +
  margin-right: 5px;
 +
  border-radius: 2px;
 +
  color: #999;
 +
  display: inline-block;
 +
  cursor: pointer;
 +
  margin-top: 5px;
 +
}
 +
.wrapper {
 +
  width: 960px;
 +
  margin: 0px auto;
 +
  overflow: hidden;
 +
  word-wrap: break-word;
 +
}
 +
/* Buttons */
 +
.button {
 +
  background: #48C7C1;
 +
  border-radius: 2px;
 +
  padding: 15px 0px 15px 0px;
 +
  width: 100%;
 +
  color: #fff;
 +
  font-size: 90%;
 +
  text-shadow: 0px 1px 2px rgba(0, 0, 0, 0.1);
 +
  cursor: pointer;
 +
  font-weight: 400;
 +
}
-
body {
 
-
    margin: 10px 0 0 0;
 
-
    padding: 0;}
 
-
#top-section {
 
-
    width: 965px;
 
-
    height: 0;
 
-
    margin: 0 auto;
 
-
    padding: 0;
 
-
    border: none;}
 
-
#menubar {
 
-
    font-size: 65%;
 
-
    top: -14px;}
 
-
.left-menu:hover {
 
-
    background-color: transparent;}
 
-
#menubar li a {
 
-
    background-color: transparent;}
 
-
#menubar:hover {
 
-
    color: white;}
 
-
#menubar li a {
 
-
    color: transparent;}
 
-
#menubar:hover li a {
 
-
    color: #000000 ;}
 
 +
/* Wrapper Sections */
 +
.wrapper.container {
 +
  background: #fff;
 +
  padding: 30px;
 +
  box-shadow: 0px 2px 5px rgba(0, 0, 0, 0.1);
 +
  margin-bottom: 30px;
 +
  border-radius: 2px;
 +
}
 +
.wrapper.footer {
 +
  background: #222;
 +
  padding: 30px;
 +
  border-radius: 2px 2px 0px 0px;
 +
}
 +
.wrapper.footer ul li a {
 +
  color: #999;
 +
}
 +
/* Columns */
 +
.col {
 +
  float: left;
 +
}
 +
.col.quarter {
 +
  width: 25%;
 +
}
 +
.col.threeQuarter {
 +
  width: 75%;
 +
}
 +
.col.border {
 +
  min-height: 200px;
 +
  border-right: 1px solid #ddd;
 +
  padding-right: 30px;
 +
}
 +
.col.padding {
 +
  padding-left: 30px;
 +
}
 +
/* Sections */
 +
.section {
 +
  padding-bottom: 15px;
 +
  margin-bottom: 15px;
 +
  border-bottom: 1px solid #ddd;
 +
}
-
#cssmenu ul,
+
.section.last {
-
#cssmenu li,
+
  border: 0;
-
#cssmenu span,
+
-
#cssmenu a {
+
   margin: 0;
   margin: 0;
   padding: 0;
   padding: 0;
-
  position: relative;
 
}
}
-
#cssmenu {
+
 
-
   height: 49px;
+
.section.price p {
-
  border-radius: 5px 5px 0 0;
+
   text-align: center;
-
  -moz-border-radius: 5px 5px 0 0;
+
-
  -webkit-border-radius: 5px 5px 0 0;
+
-
  background: #141414;
+
-
  background:url(data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAAEAAAAxCAIAAACUDVRzAAAAA3NCSVQICAjb4U/gAAAALElEQVQImWMwMrJi+v//PxMDw3+m//8ZoPR/qBgDEhuXGLoeYswhXg8R5gAAdVpfoJ3dB5oAAAAASUVORK5CYII=) 100% 100%;
+
-
  background: -moz-linear-gradient(top, #32323a 0%, #141414 100%);
+
-
  background: -webkit-gradient(linear, left top, left bottom, color-stop(0%, #32323a), color-stop(100%, #141414));
+
-
  background: -webkit-linear-gradient(top, #32323a 0%, #141414 100%);
+
-
  background: -o-linear-gradient(top, #32323a 0%, #141414 100%);
+
-
  background: -ms-linear-gradient(top, #32323a 0%, #141414 100%);
+
-
  background: linear-gradient(to bottom, #32323a 0%, #141414 100%);
+
-
  border-bottom: 2px solid #e00f16;
+
-
}
+
-
#cssmenu:after,
+
-
#cssmenu ul:after {
+
-
  content: '';
+
   display: block;
   display: block;
-
   clear: both;
+
   font-size: 120%;
}
}
-
#cssmenu a {
+
 
-
  background: #141414;
+
/* Gallery */
-
  background:url(data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAAEAAAAxCAIAAACUDVRzAAAAA3NCSVQICAjb4U/gAAAALElEQVQImWMwMrJi+v//PxMDw3+m//8ZoPR/qBgDEhuXGLoeYswhXg8R5gAAdVpfoJ3dB5oAAAAASUVORK5CYII=) 100% 100%;
+
.section.gallery {
-
  background: -moz-linear-gradient(top, #32323a 0%, #141414 100%);
+
   overflow: hidden;
-
   background: -webkit-gradient(linear, left top, left bottom, color-stop(0%, #32323a), color-stop(100%, #141414));
+
   padding: 0px 0px 0px 15px;
-
  background: -webkit-linear-gradient(top, #32323a 0%, #141414 100%);
+
-
  background: -o-linear-gradient(top, #32323a 0%, #141414 100%);
+
-
  background: -ms-linear-gradient(top, #32323a 0%, #141414 100%);
+
-
  background: linear-gradient(to bottom, #32323a 0%, #141414 100%);
+
-
  color: #ffffff;
+
-
  display: inline-block;
+
-
  font-family: Helvetica, Arial, Verdana, sans-serif;
+
-
  font-size: 12px;
+
-
  line-height: 49px;
+
-
   padding: 0 20px;
+
-
  text-decoration: none;
+
}
}
-
#cssmenu ul {
+
 
-
  list-style: none;
+
.gallery img {
-
}
+
-
#cssmenu > ul {
+
   float: left;
   float: left;
 +
  width: 33.33%;
 +
  padding: 0px 15px 15px 0px;
 +
  cursor: pointer;
}
}
-
#cssmenu > ul > li {
+
 
-
  float: left;
+
/* Media */
-
}
+
@media all and (max-width: 960px) and (min-width: 800px) {
-
#cssmenu > ul > li:hover:after {
+
   .wrapper {
-
  content: '';
+
    width: auto;
-
  display: block;
+
   }
-
  width: 0;
+
-
  height: 0;
+
-
  position: absolute;
+
-
  left: 50%;
+
-
  bottom: 0;
+
-
  border-left: 10px solid transparent;
+
-
  border-right: 10px solid transparent;
+
-
  border-bottom: 10px solid #e00f16;
+
-
  margin-left: -10px;
+
-
}
+
-
#cssmenu > ul > li:first-child > a {
+
-
  border-radius: 5px 0 0 0;
+
-
  -moz-border-radius: 5px 0 0 0;
+
-
  -webkit-border-radius: 5px 0 0 0;
+
-
}
+
-
#cssmenu > ul > li:last-child > a {
+
-
  border-radius: 0 5px 0 0;
+
-
  -moz-border-radius: 0 5px 0 0;
+
-
  -webkit-border-radius: 0 5px 0 0;
+
-
}
+
-
#cssmenu > ul > li.active > a {
+
-
  box-shadow: inset 0 0 3px #000000;
+
-
  -moz-box-shadow: inset 0 0 3px #000000;
+
-
  -webkit-box-shadow: inset 0 0 3px #000000;
+
-
  background: #070707;
+
-
  background:url(data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAAEAAAAxCAIAAACUDVRzAAAAA3NCSVQICAjb4U/gAAAALklEQVQImWNQU9Nh+v//PxMDw3+m//8ZkNj/mRgYIHxy5f//Z0BSi18e2TwS5QG4MGB54HL+mAAAAABJRU5ErkJggg==) 100% 100%;
+
-
  background: -moz-linear-gradient(top, #26262c 0%, #070707 100%);
+
-
  background: -webkit-gradient(linear, left top, left bottom, color-stop(0%, #26262c), color-stop(100%, #070707));
+
-
  background: -webkit-linear-gradient(top, #26262c 0%, #070707 100%);
+
-
  background: -o-linear-gradient(top, #26262c 0%, #070707 100%);
+
-
  background: -ms-linear-gradient(top, #26262c 0%, #070707 100%);
+
-
  background: linear-gradient(to bottom, #26262c 0%, #070707 100%);
+
-
}
+
-
#cssmenu > ul > li:hover > a {
+
-
   background: #070707;
+
-
  background:url(data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAAEAAAAxCAIAAACUDVRzAAAAA3NCSVQICAjb4U/gAAAALklEQVQImWNQU9Nh+v//PxMDw3+m//8ZkNj/mRgYIHxy5f//Z0BSi18e2TwS5QG4MGB54HL+mAAAAABJRU5ErkJggg==) 100% 100%;
+
-
  background: -moz-linear-gradient(top, #26262c 0%, #070707 100%);
+
-
  background: -webkit-gradient(linear, left top, left bottom, color-stop(0%, #26262c), color-stop(100%, #070707));
+
-
  background: -webkit-linear-gradient(top, #26262c 0%, #070707 100%);
+
-
  background: -o-linear-gradient(top, #26262c 0%, #070707 100%);
+
-
  background: -ms-linear-gradient(top, #26262c 0%, #070707 100%);
+
-
  background: linear-gradient(to bottom, #26262c 0%, #070707 100%);
+
-
  box-shadow: inset 0 0 3px #000000;
+
-
  -moz-box-shadow: inset 0 0 3px #000000;
+
-
  -webkit-box-shadow: inset 0 0 3px #000000;
+
-
}
+
-
#cssmenu .has-sub {
+
-
  z-index: 1;
+
-
}
+
-
#cssmenu .has-sub:hover > ul {
+
-
  display: block;
+
-
}
+
-
#cssmenu .has-sub ul {
+
-
  display: none;
+
-
  position: absolute;
+
-
  width: 200px;
+
-
   top: 100%;
+
-
  left: 0;
+
-
}
+
-
#cssmenu .has-sub ul li {
+
-
  *margin-bottom: -1px;
+
-
}
+
-
#cssmenu .has-sub ul li a {
+
-
  background: #e00f16;
+
-
  border-bottom: 1px dotted #ec6f73;
+
-
  filter: none;
+
-
  font-size: 11px;
+
-
  display: block;
+
-
  line-height: 120%;
+
-
  padding: 10px;
+
-
}
+
-
#cssmenu .has-sub ul li:hover a {
+
-
  background: #b00c11;
+
-
}
+
-
#cssmenu .has-sub .has-sub:hover > ul {
+
-
  display: block;
+
-
}
+
-
#cssmenu .has-sub .has-sub ul {
+
-
  display: none;
+
-
  position: absolute;
+
-
  left: 100%;
+
-
  top: 0;
+
-
}
+
-
#cssmenu .has-sub .has-sub ul li a {
+
-
  background: #b00c11;
+
-
  border-bottom: 1px dotted #d06d70;
+
-
}
+
-
#cssmenu .has-sub .has-sub ul li a:hover {
+
-
  background: #80090d;
+
}
}
 +
@media all and (max-width: 650px) {
 +
  .wrapper {
 +
    width: 650px;
 +
  }
 +
}<script>
 +
$(function(){
 +
  $('.gallery img').click(function(){
 +
    $('.itemImg').attr('src', $(this).attr('src'));
 +
  });
 +
})
 +
</script>
</style>
</style>
</head>
</head>
-
<link href="/menu_assets/styles.css" rel="stylesheet" type="text/css">
+
<body>
-
<link href="../Downloads/cssmenu-1/menu_assets/styles.css" rel="stylesheet" type="text/css">
+
 
-
<div id='cssmenu'>
+
 
-
<ul>
+
 
-
  <li class='active'><a href='https://2013.igem.org/Team:Glendale_CC_AZ'><span>Home</span></a></li>
+
 
-
  <li class='has-sub'><a href='https://2013.igem.org/Team:Glendale_CC_AZ/Team/'><span>Team</span></a>
+
 
-
      <ul>
+
 
-
        <li><a href='https://2013.igem.org/Team:Glendale_CC_AZ/Team/students'><span>Students</span></a></li>
+
 
-
        <li><a href='https://2013.igem.org/Team:Glendale_CC_AZ/Team/Instuctors'><span>Instructors</span></a></li>
+
 
-
        <li><a href='https://2013.igem.org/Team:Glendale_CC_AZ/Gallery'><span>Gallery</span></a></li>
+
<div class="wrapper container">
-
        <li class='last'><a href='https://igem.org/Team.cgi?year=2013&team_name=Glendale_CC_AZ'><span>Official Team Profile</span></a></li>
+
  <div class="col threeQuarter border">
-
      </ul>
+
    <div class="section">
-
  </li>
+
      <h2>Abstract</h2>
-
  <li class='has-sub'><a href='https://2013.igem.org/Team:Glendale_CC_AZ/Project'><span>Project</span></a>
+
      <img src="https://static.igem.org/mediawiki/igem.org/1/19/Tubes2GCC.JPG" draggable="false" class="itemImg" />
-
      <ul>
+
    </div>
-
        <li class='has-sub'><a href='https://2013.igem.org/Team:Glendale_CC_AZ/Project/Background'><span>Background</span></a>
+
   
-
            <ul>
+
    <div class="section gallery">
-
              <li><a href='https://2013.igem.org/Team:Glendale_CC_AZ/Project/Background/Overview'><span>Overview</span></a></li>
+
      <img src="https://static.igem.org/mediawiki/igem.org/5/59/ExperimentGCC.JPG" draggable="false" />
-
              <li><a href='https://2013.igem.org/Team:Glendale_CC_AZ/Project/Background/Overview/GrowingDeserts'><span>Growing Deserts</span></a></li>
+
      <img src="https://static.igem.org/mediawiki/igem.org/7/7e/Lab1GCC.JPG" draggable="false" />
-
              <li class='last'><a href='https://2013.igem.org/Team:Glendale_CC_AZ/Project/Background/Overview/ThreatenDeserts'><span>Threaten Deserts</span></a></li>
+
    </div>
-
            </ul>
+
   
-
        </li>
+
    <div class="section last">
-
        <li class='has-sub'><a href='https://2013.igem.org/Team:Glendale_CC_AZ/Project/Wetlab'><span>Wet Lab</span></a>
+
      <p>These are some pictures from the experiments performes during the Summer 2013</p>
-
            <ul>
+
    </div>
-
              <li><a href='https://2013.igem.org/Team:Glendale_CC_AZ/Wetlab/Overview'><span>Overview</span></a></li>
+
   
-
              <li><a href='https://2013.igem.org/Team:Glendale_CC_AZ/Wetlab/Overview/Experiments'><span>Experiments</span></a></li>
+
    <div class="content-area">
-
              <li class='last'><a href='https://2013.igem.org/Team:Glendale_CC_AZ/Wetlab/Overview/Experiments/Results'><span>Results</span></a></li>
+
  <article>
-
            </ul>
+
    <h3>Previous Research</h3>
-
        </li>
+
    <h3>Deinococcus radiodurans</h3>
-
        <li><a href='https://2013.igem.org/Team:Glendale_CC_AZ/Human_Practices'><span>Human Practices</span></a></li>
+
    <p>Lorem ipsum dolor sit amet, consectetur adipiscing elit. Vivamus magna. Cras in mi at felis aliquet congue. Ut a est eget ligula molestie gravida. Curabitur massa. Donec eleifend, libero at sagittis mollis, tellus est malesuada tellus, at luctus turpis elit sit amet quam. Vivamus pretium ornare est.  
-
        <li><a href='https://2013.igem.org/Team:Glendale_CC_AZ/Safety'><span>Safety</span></a></li>
+
   
-
        <li class='last'><a href='https://2013.igem.org/Team:Glendale_CC_AZ/Achievments'><span>Achievements</span></a></li>
+
    <h3>PprI</h3>
-
      </ul>
+
   
-
  </li>
+
      <p> For the team’s Igem project, we wanted to increase E. coli’s desiccation resistance to help it survive in a desert environment. To do this, we planned to insert different genes from the <em>Deinococcus</em> species into the <em>E. coli</em> to increase its resistance to dessication. One of the genes our Igem group wanted to investigate was PprI. PprI is the transcriptional regulator involved in the expression of many of the DNA damage response proteins in  <em>Deinococcus radiodurans.</em> In 2012, the Osaka team transformed E. coli with PprI to determine its effects on hydrogen peroxide resistance. Because of this, a PprI part from this team was in the Igem parts database. This allowed us to order their PprI part from the Igem registry and perform our experiments with it.</p>
-
  <li class='has-sub'><a href='https://2013.igem.org/Team:Glendale_CC_AZ/Notebook'><span>Notebook</span></a>
+
   
-
       <ul>
+
    <h3>LeA</h3>
-
        <li class='last'><a href='https://2013.igem.org/Team:Glendale_CC_AZ/Notebook/Calendar'><span>Calendar of Events</span></a></li>
+
   
-
      </ul>
+
    <p>During our research for desiccation-resistance factors, we came across with the 2010 Valencia iGEM project. They demonstrated that late embryogenesis abundant proteins (LEA) from soy beans provided protection against extreme temperatures, when expressed in E. coli. Since high temperatures might lead to desiccation, we decided to explore LEA proteins in more detail. Previous studies have reported that LEA protein enhances the tolerance to various environmental stresses in organisms (Liu, Zheng, Zhang, Wang, & Li, 2010). They were first discovered in cottonseed and other plants vegetative tissues that were exposed to cold temperatures, drought, and high salinity (Liu et al., 2010).</p>  
-
  </li>
+
    <blockquote>
-
  <li class='active'><a href='https://2013.igem.org/Team:Glendale_CC_AZ/Protocols'><span>Protocols</span></a></li>
+
      <aside>"At first, the idea of plant proteins in <em>Deinococcus</em> sounded unconceivable to us. However, our curiosity paid off as we discovered that <em>Deinococcus radiodurans</em> had homolog LEA proteins which are hypothesized to be acquired via horizontal gene transfer (Makarova et al., 2001). Three of these D. radiodurans proteins are DR0105, DR1372 and DR1172 which show similarity to well-characterized and widespread desiccation induced LEA proteins in plants (Makarova et al., 2001)."<cite> -GCC's iGEM Team</cite></aside>   </blockquote>
-
  <li class='active'><a href='https://2013.igem.org/Team:Glendale_CC_AZ/Parts'><span>Submitted Parts</span></a></li>
+
     
-
  <li class='active has-sub last'><a href='https://2013.igem.org/Team:Glendale_CC_AZ/Attributions'><span>Attributions</span></a>
+
      LEA proteins are part of an extensive multi-gene family and are classified based on their sequences and expression patterns (Liu et al., 2010). Since Deinococcus was our organism of interest, we looked for studies that had reported homolog LEA proteins in this bacterium. </p>
-
       <ul>
+
    <h3>PprA</h3>
-
        <li class='active'><a href='https://2013.igem.org/Team:Glendale_CC_AZ/Attributions/Sponsors'><span>Sponsors</span></a></li>
+
   
-
        <li><a href='https://2013.igem.org/Team:Glendale_CC_AZ/Attributions/Acknowledgments'><span>Acknowledgments</span></a></li>
+
    <p>Pellentesque habitant morbi tristique senectus et netus et malesuada fames ac turpis egestas. Vestibulum tortor quam, feugiat vitae, ultricies eget, tempor sit amet, ante. Donec eu libero sit amet quam egestas semper. facilisis luctus, metus</p>
-
        <li class='last'><a href='https://2013.igem.org/Team:Glendale_CC_AZ/Attributions/Outreach'><span>Outreach</span></a></li>
+
   
-
       </ul>
+
    <p>Pellentesque habitant morbi tristique senectus et netus et malesuada fames ac turpis egestas. Vestibulum tortor quam, feugiat vitae, ultricies eget, tempor sit amet, ante. Donec eu libero sit amet quam egestas semper. facilisis luctus, metus</p>
-
  </li>
+
   
-
</ul>
+
 
 +
   
 +
    <h3>RecA</h3>
 +
    <p>Lorem ipsum dolor sit amet, consectetur adipiscing elit. Vivamus magna. Cras in mi at felis aliquet congue. Ut a est eget ligula molestie gravida. Curabitur massa. Donec eleifend, libero at sagittis mollis, tellus est malesuada tellus, at luctus turpis elit sit amet quam. Vivamus pretium ornare est.</p>
 +
   
 +
    <h3>PprM</h3>
 +
    <p>Lorem ipsum dolor sit amet, consectetur adipiscing elit. Vivamus magna. Cras in mi at felis aliquet congue. Ut a est eget ligula molestie gravida. Curabitur massa. Donec eleifend, libero at sagittis mollis, tellus est malesuada tellus, at luctus turpis elit sit amet quam. Vivamus pretium ornare est.</p>
 +
    <hr />
 +
    <h3>References</h3>
 +
   
 +
    <ul>
 +
      <li>Liu, Y., Zheng, Y., Zhang, Y., Wang, W., & Li, R. (2010, May 1). Soybean PM2 protein (LEA3) confers the tolerance of Escherichia coli and stabilization of enzyme activity under diverse stresses. Current Microbiology, 60(5), 373-378. doi:10.1007/s00284-009-9552-2
 +
 
 +
</li>
 +
       <li>MAKAROVA, K. S., ARAVIND, L., WOLF, Y. I., TATUSOV, R., MINTON, K. W., KOONIN, E. V., & DALY, M. J. (2001, March).
 +
Genome of the extremely radiation-resistant bacterium Deinococcus radiodurans viewed from the perspective of comparative genomics. MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 65(1), 44-79. doi:10.1128/MMBR.65.1.44–79.2001</li>
 +
    </ul>  
 +
  </article>
 +
</div><!--/.content-area-->
 +
   
 +
   
 +
   
 +
   
 +
   
 +
  </div>
 +
 
 +
  <div class="col quarter padding">
 +
    <div class="section">
 +
    <p>Cool equire a shoe that you can trust, like the Nike Air Max 1 Running Shoes. The legendary status of this classic running shoe is not all hype, as anyone who has worn these premium running kicks can attest. Get the support, comfort and style you need from these iconic running shoes.</p>
 +
      <p><a href="#">More products like this</a></p>
 +
       <p class="tags"><a href="#">Info</a><a href="#">More</a><a href="#">EX</a></p>
 +
     
 +
     
 +
     
 +
    </div>
 +
   
 +
 
 +
   
 +
    <div class="section last">
 +
       <input type="submit" value="Add to Basket" class="button" />
 +
    </div>
 +
  </div>
</div>
</div>
 +
 +
 +
 
 +
 +
</body>
</html>
</html>

Revision as of 21:34, 21 September 2013

Untitled Document

Abstract

These are some pictures from the experiments performes during the Summer 2013

Previous Research

Deinococcus radiodurans

Lorem ipsum dolor sit amet, consectetur adipiscing elit. Vivamus magna. Cras in mi at felis aliquet congue. Ut a est eget ligula molestie gravida. Curabitur massa. Donec eleifend, libero at sagittis mollis, tellus est malesuada tellus, at luctus turpis elit sit amet quam. Vivamus pretium ornare est.

PprI

For the team’s Igem project, we wanted to increase E. coli’s desiccation resistance to help it survive in a desert environment. To do this, we planned to insert different genes from the Deinococcus species into the E. coli to increase its resistance to dessication. One of the genes our Igem group wanted to investigate was PprI. PprI is the transcriptional regulator involved in the expression of many of the DNA damage response proteins in Deinococcus radiodurans. In 2012, the Osaka team transformed E. coli with PprI to determine its effects on hydrogen peroxide resistance. Because of this, a PprI part from this team was in the Igem parts database. This allowed us to order their PprI part from the Igem registry and perform our experiments with it.

LeA

During our research for desiccation-resistance factors, we came across with the 2010 Valencia iGEM project. They demonstrated that late embryogenesis abundant proteins (LEA) from soy beans provided protection against extreme temperatures, when expressed in E. coli. Since high temperatures might lead to desiccation, we decided to explore LEA proteins in more detail. Previous studies have reported that LEA protein enhances the tolerance to various environmental stresses in organisms (Liu, Zheng, Zhang, Wang, & Li, 2010). They were first discovered in cottonseed and other plants vegetative tissues that were exposed to cold temperatures, drought, and high salinity (Liu et al., 2010).

LEA proteins are part of an extensive multi-gene family and are classified based on their sequences and expression patterns (Liu et al., 2010). Since Deinococcus was our organism of interest, we looked for studies that had reported homolog LEA proteins in this bacterium.

PprA

Pellentesque habitant morbi tristique senectus et netus et malesuada fames ac turpis egestas. Vestibulum tortor quam, feugiat vitae, ultricies eget, tempor sit amet, ante. Donec eu libero sit amet quam egestas semper. facilisis luctus, metus

Pellentesque habitant morbi tristique senectus et netus et malesuada fames ac turpis egestas. Vestibulum tortor quam, feugiat vitae, ultricies eget, tempor sit amet, ante. Donec eu libero sit amet quam egestas semper. facilisis luctus, metus

RecA

Lorem ipsum dolor sit amet, consectetur adipiscing elit. Vivamus magna. Cras in mi at felis aliquet congue. Ut a est eget ligula molestie gravida. Curabitur massa. Donec eleifend, libero at sagittis mollis, tellus est malesuada tellus, at luctus turpis elit sit amet quam. Vivamus pretium ornare est.

PprM

Lorem ipsum dolor sit amet, consectetur adipiscing elit. Vivamus magna. Cras in mi at felis aliquet congue. Ut a est eget ligula molestie gravida. Curabitur massa. Donec eleifend, libero at sagittis mollis, tellus est malesuada tellus, at luctus turpis elit sit amet quam. Vivamus pretium ornare est.

References

  • Liu, Y., Zheng, Y., Zhang, Y., Wang, W., & Li, R. (2010, May 1). Soybean PM2 protein (LEA3) confers the tolerance of Escherichia coli and stabilization of enzyme activity under diverse stresses. Current Microbiology, 60(5), 373-378. doi:10.1007/s00284-009-9552-2
  • MAKAROVA, K. S., ARAVIND, L., WOLF, Y. I., TATUSOV, R., MINTON, K. W., KOONIN, E. V., & DALY, M. J. (2001, March). Genome of the extremely radiation-resistant bacterium Deinococcus radiodurans viewed from the perspective of comparative genomics. MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 65(1), 44-79. doi:10.1128/MMBR.65.1.44–79.2001

Cool equire a shoe that you can trust, like the Nike Air Max 1 Running Shoes. The legendary status of this classic running shoe is not all hype, as anyone who has worn these premium running kicks can attest. Get the support, comfort and style you need from these iconic running shoes.

More products like this

InfoMoreEX

Retrieved from "http://2013.igem.org/Team:Glendale_CC_AZ/tests"