Team:Grenoble-EMSE-LSU/Project/Monitoring/Cell2Machine

From 2013.igem.org

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<p>Before measuring with the photodiode, we need to know if the photodiode works as indicated in the datasheet. The photodiode was plug on a 5V stabilized power supply. For the same amount of light, we measure the frequency at the output of the photodiode for a pulse train or a square wave (50% duty cycle). According to the datasheet, when using a pulse train the linear relation between the frequency and the irrandiance is given by 1kHz=1µW/cm². When using a square wave (50% duty cycle) it is 1kHz=2µW/cm². This is what we can see on the figure #.
<p>Before measuring with the photodiode, we need to know if the photodiode works as indicated in the datasheet. The photodiode was plug on a 5V stabilized power supply. For the same amount of light, we measure the frequency at the output of the photodiode for a pulse train or a square wave (50% duty cycle). According to the datasheet, when using a pulse train the linear relation between the frequency and the irrandiance is given by 1kHz=1µW/cm². When using a square wave (50% duty cycle) it is 1kHz=2µW/cm². This is what we can see on the figure #.
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                                        <img href="https://static.igem.org/mediawiki/2013/5/51/Oscilloscope.png">  
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<img href="https://static.igem.org/mediawiki/2013/5/51/Oscilloscope.png"/>  
                                         <p>Since this frequency will be calculated by the Arduino controller, it may cause some trouble to the program to use a pulse train because the duration of the pulse is always 500ns and can be missed by the controller. The square wave (50% duty cycle) seems to be a better solution because of the 50% duty cycle. It means that the pulse duration depend on the frequency. Its duration is equal to 1/2f and since the light intensity we want to measure will be low this type of signal can be easily detected by Arduino.</p>
                                         <p>Since this frequency will be calculated by the Arduino controller, it may cause some trouble to the program to use a pulse train because the duration of the pulse is always 500ns and can be missed by the controller. The square wave (50% duty cycle) seems to be a better solution because of the 50% duty cycle. It means that the pulse duration depend on the frequency. Its duration is equal to 1/2f and since the light intensity we want to measure will be low this type of signal can be easily detected by Arduino.</p>
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Revision as of 14:39, 2 September 2013

Grenoble-EMSE-LSU, iGEM


Grenoble-EMSE-LSU, iGEM

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