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Team:Groningen/20 June 2013
From 2013.igem.org
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| - | Added 1 ul Serva/100 ml 1.5% agarose gel. | + | </b>Added 1 ul Serva/100 ml 1.5% agarose gel. |
| - | Made a dilution for the Fermentas 1 kb GeneRuler: added 150 ul 6x loading buffer and 750 ul MQ water. | + | </b>Made a dilution for the Fermentas 1 kb GeneRuler: added 150 ul 6x loading buffer and 750 ul MQ water. |
| - | Made a 2x dilution of the loading buffer. | + | </b>Made a 2x dilution of the loading buffer. |
The restriction analysis of BBa_K844016 is done with EcoRI, XbaI, SpeI, PstI and BfaI. The following restriction solution is made: | The restriction analysis of BBa_K844016 is done with EcoRI, XbaI, SpeI, PstI and BfaI. The following restriction solution is made: | ||
Revision as of 14:33, 21 June 2013
</b>Added 1 ul Serva/100 ml 1.5% agarose gel. </b>Made a dilution for the Fermentas 1 kb GeneRuler: added 150 ul 6x loading buffer and 750 ul MQ water. </b>Made a 2x dilution of the loading buffer.
The restriction analysis of BBa_K844016 is done with EcoRI, XbaI, SpeI, PstI and BfaI. The following restriction solution is made: 7 ul MQ water 1 ul 10x buffer 1 ul restriction enzyme 2 ul DNA solution
The incubation is done at 37 degrees Celsius for 2 hours.
After incubation the restricted BBa_K844016, 2.5 ul 2x loading buffer + 3.5 ul restricted biobrick, is loaded on gel. The gel electrophorese is done for 25 minutes at 90V.
The picture is coming soon.
