Team:Groningen/20 June 2013

From 2013.igem.org

(Difference between revisions)
(Created page with "Started the restriction digest on biobrick BBa_K844016, <br>EcoRI<br/> <br>XbaI<br/> <br>SpeI<br/> <br>PstI<br/> <br>BfaI<br/> 10:59 put tubes in in 37 C incubator")
 
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Started the restriction digest on biobrick BBa_K844016,
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'''Mirjam, Inne and Erik'''
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<br/>Added 1 ul Serva/100 ml 1.5% agarose gel.
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<br/>Made a dilution for the Fermentas 1 kb GeneRuler: added 150 ul 6x loading buffer and 750 ul MQ water.
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<br/>Made a 2x dilution of the loading buffer.
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<br>EcoRI<br/>
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The restriction analysis of BBa_K844016 is done with EcoRI, XbaI, SpeI, PstI and BfaI. The following restriction solution is made:
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<br>XbaI<br/>
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<br/>7 ul MQ water
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<br>SpeI<br/>
+
<br/>1 ul 10x buffer
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<br>PstI<br/>
+
<br/>1 ul restriction enzyme
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<br>BfaI<br/>
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<br/>2 ul DNA solution
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10:59 put tubes in in 37 C incubator
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The incubation is done at 37 degrees Celsius for 2 hours.
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After incubation the restricted BBa_K844016, 2.5 ul 2x loading buffer + 3.5 ul restricted biobrick, is loaded on gel.
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<br/>The gel electrophorese is done for 25 minutes at 90V.
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The picture is coming soon.

Latest revision as of 11:12, 28 June 2013

Mirjam, Inne and Erik
Added 1 ul Serva/100 ml 1.5% agarose gel.
Made a dilution for the Fermentas 1 kb GeneRuler: added 150 ul 6x loading buffer and 750 ul MQ water.
Made a 2x dilution of the loading buffer.

The restriction analysis of BBa_K844016 is done with EcoRI, XbaI, SpeI, PstI and BfaI. The following restriction solution is made:
7 ul MQ water
1 ul 10x buffer
1 ul restriction enzyme
2 ul DNA solution

The incubation is done at 37 degrees Celsius for 2 hours.

After incubation the restricted BBa_K844016, 2.5 ul 2x loading buffer + 3.5 ul restricted biobrick, is loaded on gel.
The gel electrophorese is done for 25 minutes at 90V.

The picture is coming soon.