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Team:Groningen/27 June 2013
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Revision as of 11:03, 27 June 2013
Today the PCR products of the signal sequences (FliZ, EstA, MotB and LytB) are purified using gel purification.
A PCR is done for some different silk constructs to start with. The following combinations are made:
1) signal sequence - N-terminal strep tag - silk
2) signal sequence - silk - C-terminal strep tag
3) signal sequence - silk - no strep tag
4) silk without tag
The following protocol is used:
