Team:Groningen/27 June 2013

From 2013.igem.org

Revision as of 10:59, 27 June 2013 by Mirjam (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Today the PCR products of the signal sequences (FliZ, EstA, MotB and LytB) are purified using gel purification.

A PCR is done for some different silk constructs to start with. The following combinations are made:
1) signal sequence - N-terminal strep tag - silk
2) signal sequence - silk - C-terminal strep tag
3) signal sequence - silk - no strep tag
4) silk without tag