Team:Groningen/27 June 2013


Revision as of 11:03, 27 June 2013 by Mirjam (Talk | contribs)

Today the PCR products of the signal sequences (FliZ, EstA, MotB and LytB) are purified using gel purification.

A PCR is done for some different silk constructs to start with. The following combinations are made:
1) signal sequence - N-terminal strep tag - silk
2) signal sequence - silk - C-terminal strep tag
3) signal sequence - silk - no strep tag
4) silk without tag

The following protocol is used: