Team:Groningen/4 July 2013

From 2013.igem.org

(Difference between revisions)
Line 7: Line 7:
Ran a gel with the 8 PCR products and the purified promoter from yesterday (as a check).
Ran a gel with the 8 PCR products and the purified promoter from yesterday (as a check).
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<br/>Well 1 2 3 4 5 6 7 8 9 10
+
<table>
-
<br/>promoter Marker 1 1. 2 2. 3 3. 4 4.
+
<tr>
-
 
+
<th>Well</th>
 +
<th>1</th>
 +
<th>2</th>
 +
<th>3</th>
 +
<th>4</th>
 +
<th>5</th>
 +
<th>6</th>
 +
<th>7</th>
 +
<th>8</th>
 +
<th>9</th>
 +
<th> 10</th>
 +
</tr>
 +
<tr>
 +
<td></td>
 +
<td>promoter</td>
 +
<td>Marker</td>
 +
<td>1</td>
 +
<td>1.</td>
 +
<td>2</td>
 +
<td>2.</td>
 +
<td>3</td>
 +
<td>3.</td>
 +
<td>4</td>
 +
<td>4.</td>
 +
</tr>
 +
</table>
1. = duplo of sample 1
1. = duplo of sample 1

Revision as of 12:37, 4 July 2013

Sander and Inne


For the silk it is decided to try a PCR with an annealing temperature of 85 and 90 degrees Celsius. we followed the usual protocol aside from the standard diluted template DNA but also tried undiluted template DNA 1, (ul in 50 ul mix for both of then) .
1 2 3 4 5 6
98 98 85/90 72 72 4
20:00 0:30 0:25 0:27 10:00 forever

Ran a gel with the 8 PCR products and the purified promoter from yesterday (as a check).

Well 1 2 3 4 5 6 7 8 9 10
promoter Marker 1 1. 2 2. 3 3. 4 4.

1. = duplo of sample 1

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