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Team:Groningen/4 July 2013
From 2013.igem.org
Sander and Inne
For the silk it is decided to try a PCR with an annealing temperature of 85 and 90 degrees Celsius. we followed the usual protocol aside from the standard diluted template DNA but also tried undiluted template DNA 1, (ul in 50 ul mix for both of then) .
1 2 3 4 5 6
98 98 85/90 72 72 4
20:00 0:30 0:25 0:27 10:00 forever
Ran a gel with the 8 PCR products and the purified promoter from yesterday (as a check).
| Well | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
|---|---|---|---|---|---|---|---|---|---|---|
| promoter | Marker | 1 | 1. | 2 | 2. | 3 | 3. | 4 | 4. |
1. = duplo of sample 1
Ran the same gel again for 22 min on 100 volt to get a better picture
(picture was take about an hour and a half after run finished because of our meeting)
