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From 2013.igem.org

Mirjam
PCR made for silk without strep-tags.
Gel run for 22 min 90V.
Gel reveals a small band at 900bp. That is why a new 1.5% agarosegel is run for 30 min at 90V.
Cut wrong bands from gel. So a new PCR is made.

Inne

spotted transmuted cells and put them in a 37 C shaker (at 11:35) Cells need to grow over night.

15:00 PCR in order to get more silk gene to cut out of gel(last one was cut to low)