Team:Groningen/Lab/experiments/Biofilm
From 2013.igem.org
(Difference between revisions)
(Created page with "<html> <h1>Biofilm Growth on different materials</h1> <p> To find out if the <i>Bacillus subtilis</i> strain used can form a biofilm on material often used in implants, an experi...") |
|||
(4 intermediate revisions not shown) | |||
Line 1: | Line 1: | ||
<html> | <html> | ||
+ | |||
+ | <!-- | ||
+ | |||
+ | INSTRUCTIONS: If you want to edit the content of this page please go to: https://2013.igem.org/Team:Groningen/Lab/experiments | ||
+ | DO NOT EDIT THIS PAGE | ||
+ | |||
+ | --> | ||
+ | |||
+ | |||
+ | |||
+ | <head> | ||
+ | <meta http-equiv="X-UA-Compatible" content="IE=EDGE" charset="utf-8" /> | ||
+ | <link rel="stylesheet" href="https://2013.igem.org/Team:Groningen/CSS/DefaultPage?action=raw&ctype=text/css" type="text/css" /> | ||
+ | </head> | ||
+ | <body> | ||
+ | |||
+ | </html> {{:Team:Groningen/Templates/DefaultHeader}}<html> | ||
+ | |||
+ | <div class="colmask threecol"> | ||
+ | |||
+ | <span class="navigation"> | ||
+ | </html>{{:Team:Groningen/Templates/Navigationbar}}<html> | ||
+ | </span> | ||
+ | |||
+ | <span class="widgets"> | ||
+ | </html>{{:Team:Groningen/Templates/facebook}}<html> | ||
+ | </span> | ||
+ | |||
+ | <div class="mainContent"> | ||
<h1>Biofilm Growth on different materials</h1> | <h1>Biofilm Growth on different materials</h1> | ||
+ | |||
+ | To find out if the <i>Bacillus subtilis</i> strain used in this project can form a biofilm on material often used as implants, an experiment was set up. | ||
+ | |||
<p> | <p> | ||
- | + | The suggested implant material was placed in a petri dish with Sgg medium. The material is partially submerged in the medium and inoculated with the appropriate <B.subtilis</i> strain. After incubation the plate is left in a 27°C environment for 5 days. After the incubation time, the total biofilm growth on the material of interest is measured. | |
- | + | <br>Two different implant materials are tested: titanium and plastic. After five days of growth on titanium, there is 3 mm biofilm, starting at the medium growing up on titanium (Figure 1). No biofilm formation was possible on plastic (Figure 2). | |
- | + | ||
- | + | ||
- | + | ||
- | After | + | |
</p> | </p> | ||
- | + | <br> | |
<div align="left"> <!--you can use left/right or center to align the image--> | <div align="left"> <!--you can use left/right or center to align the image--> | ||
<table id="layout" width=30%> <!--change the percentage to determine the size of your image--> | <table id="layout" width=30%> <!--change the percentage to determine the size of your image--> | ||
Line 20: | Line 49: | ||
<tr> | <tr> | ||
<td> | <td> | ||
- | <font size="1">Figure 1: Titanium plate with 3 mm | + | <font size="1">Figure 1: Titanium plate with 3 mm of biofilm production.</font> |
</td> | </td> | ||
</tr> | </tr> | ||
Line 37: | Line 66: | ||
<tr> | <tr> | ||
<td> | <td> | ||
- | <font size="1">Figure 2: | + | <font size="1">Figure 2: No biofilm production was seen on plastic.</font> |
</td> | </td> | ||
</tr> | </tr> | ||
Line 47: | Line 76: | ||
<p/> | <p/> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | </div> | ||
+ | |||
+ | </html> {{:Team:Groningen/Templates/DefaultFooter}}<html> | ||
+ | |||
+ | |||
+ | </body> | ||
</html> | </html> |
Latest revision as of 23:43, 4 October 2013
Biofilm Growth on different materials
To find out if the Bacillus subtilis strain used in this project can form a biofilm on material often used as implants, an experiment was set up.
The suggested implant material was placed in a petri dish with Sgg medium. The material is partially submerged in the medium and inoculated with the appropriate
Two different implant materials are tested: titanium and plastic. After five days of growth on titanium, there is 3 mm biofilm, starting at the medium growing up on titanium (Figure 1). No biofilm formation was possible on plastic (Figure 2).
Figure 1: Titanium plate with 3 mm of biofilm production. |
Figure 2: No biofilm production was seen on plastic. |