Team:Groningen/Lab/experiments/Biofilm
From 2013.igem.org
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<h1>Biofilm Growth on different materials</h1> | <h1>Biofilm Growth on different materials</h1> | ||
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+ | To find out if the <i>Bacillus subtilis</i> strain used in this project can form a biofilm on material often used as implants, an experiment was set up. | ||
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- | + | The suggested implant material was placed in a petri dish with Sgg medium. The material is partially submerged in the medium and inoculated with the appropriate <B.subtilis</i> strain. After incubation the plate is left in a 27°C environment for 5 days. After the incubation time, the total biofilm growth on the material of interest is measured. | |
- | + | <br>Two different implant materials are tested: titanium and plastic. After five days of growth on titanium, there is 3 mm biofilm, starting at the medium growing up on titanium (Figure 1). No biofilm formation was possible on plastic (Figure 2). | |
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- | <font size="1">Figure 1: Titanium plate with 3 mm | + | <font size="1">Figure 1: Titanium plate with 3 mm of biofilm production.</font> |
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- | <font size="1">Figure 2: | + | <font size="1">Figure 2: No biofilm production was seen on plastic.</font> |
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Latest revision as of 23:43, 4 October 2013
Biofilm Growth on different materials
To find out if the Bacillus subtilis strain used in this project can form a biofilm on material often used as implants, an experiment was set up.
The suggested implant material was placed in a petri dish with Sgg medium. The material is partially submerged in the medium and inoculated with the appropriate
Two different implant materials are tested: titanium and plastic. After five days of growth on titanium, there is 3 mm biofilm, starting at the medium growing up on titanium (Figure 1). No biofilm formation was possible on plastic (Figure 2).
Figure 1: Titanium plate with 3 mm of biofilm production. |
Figure 2: No biofilm production was seen on plastic. |