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Team:Groningen/Labwork/17 July 2013
From 2013.igem.org
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<br>The ''E. coli'' cells with the BBa_k818000 that were to grow overnight didn't grow. | <br>The ''E. coli'' cells with the BBa_k818000 that were to grow overnight didn't grow. | ||
<br>The tubes that were supposed to be turbid were still clear, however the negative control was also clear so that one did succeed. | <br>The tubes that were supposed to be turbid were still clear, however the negative control was also clear so that one did succeed. | ||
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Revision as of 10:14, 17 July 2013
Mirjam
Check of the restriction done yesterday. This revealed that 5/7 colonies did contain the transformant. The cells of the fourth plate started to grow, so a mini prep analysis is performed. A restriction analysis is done with EcoRV.
A second confirmation of the constructs is done using PCR.
Inne
The E. coli cells with the BBa_k818000 that were to grow overnight didn't grow.
The tubes that were supposed to be turbid were still clear, however the negative control was also clear so that one did succeed.
