Team:Groningen/Labwork/18 July 2013

From 2013.igem.org

(Difference between revisions)
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<br>Both negative controls were clear, and the rest of the samples was turbid.
<br>Both negative controls were clear, and the rest of the samples was turbid.
<br>Did a miniprep of the samples 1 & 2 (the ones with Amp).
<br>Did a miniprep of the samples 1 & 2 (the ones with Amp).
 +
<table border="1">
 +
<tr>
 +
<td>#</td>
 +
<td>composition</td>
 +
<td>Bacteria</td>
 +
</tr>
 +
<tr>
 +
<td>1</td>
 +
<td>4mL LB 8uL Amp</td>
 +
<td>+</td>
 +
</tr>
 +
<tr>
 +
<td>2</td>
 +
<td>4mL LB 8uL Amp</td>
 +
<td>+</td>
 +
</tr>
 +
<tr>
 +
<td>3</td>
 +
<td>4mL LB 8uL Amp (negative control)</td>
 +
<td>-</td>
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</tr>
 +
<tr>
 +
<td>4</td>
 +
<td>4mL LB</td>
 +
<td>+</td>
 +
</tr>
 +
<tr>
 +
<td>5</td>
 +
<td>4mL LB (negative control)</td>
 +
<td>-</td>
 +
</tr>
 +
</table>
 +
<br>Afterwards concentration of DNA was determined via nanodrop.
<br>Afterwards concentration of DNA was determined via nanodrop.

Revision as of 11:17, 18 July 2013

Sander
prepared 20 tubes for use in -80 c Glyserol stock

Inne & Mirjam
Cells with BBa_k818000, inoculated overnight, were successfully grown.
Both negative controls were clear, and the rest of the samples was turbid.
Did a miniprep of the samples 1 & 2 (the ones with Amp).

# composition Bacteria
1 4mL LB 8uL Amp +
2 4mL LB 8uL Amp +
3 4mL LB 8uL Amp (negative control) -
4 4mL LB +
5 4mL LB (negative control) -


Afterwards concentration of DNA was determined via nanodrop.


Results: sample 1 = 60.1 ng/uL, sample 2 = 31.8 ng/uL


During the miniprep the filtered LB medium was poured back into the culture (so cells could grow again).
A -80 C stock was made with 1 mL culture (sample 1). (stored in tray: iGEM 2012 A, compartment 70)

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