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Team:Groningen/Labwork/18 July 2013
From 2013.igem.org
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Revision as of 15:20, 18 July 2013
Sander
prepared 20 tubes for use in -80 c Glyserol stock.
created a restriction digest reaction for silk.
| Sample | componant | volume (ul) |
| silk1 | silk | 5 |
| BamHI buffer | 2 | |
| BamHI enzyme | 0,5 | |
| BSA | 0,5 |
MilliQ was added to reach a 20 ul volume.
placed in incubater at 37,2 C at 17:10
Inne & Mirjam
Cells with BBa_k818000, inoculated overnight, were successfully grown.
Both negative controls were clear, and the rest of the samples was turbid.
Did a miniprep of the samples 1 & 2 (the ones with Amp).
| # | composition | Bacteria |
| 1 | 4mL LB 8uL Amp | + |
| 2 | 4mL LB 8uL Amp | + |
| 3 | 4mL LB 8uL Amp (negative control) | - |
| 4 | 4mL LB | + |
| 5 | 4mL LB (negative control) | - |
Afterwards concentration of DNA was determined via nanodrop.
Results: sample 1 = 60.1 ng/uL, sample 2 = 31.8 ng/uL
During the miniprep the filtered LB medium was poured back into the culture (so cells could grow again).
A -80 C stock was made with 1 mL culture (sample 1). (stored in tray: iGEM 2012 A, compartment 70)
