"
Team:Groningen/Labwork/18 July 2013
From 2013.igem.org
(Difference between revisions)
| Line 1: | Line 1: | ||
'''Sander''' | '''Sander''' | ||
<br/>prepared 20 tubes for use in -80 c Glyserol stock | <br/>prepared 20 tubes for use in -80 c Glyserol stock | ||
| + | |||
| + | '''Inne''' | ||
| + | Cells with BBa_k818000, inoculated overnight, were successfully grown. | ||
| + | Both negative controls were clear, and the rest of the samples was turbid. | ||
| + | Did a miniprep of the samples 1 & 2 (the ones with Amp). | ||
| + | Afterwards concentration of DNA was determined via nanodrop. | ||
| + | |||
| + | Results: sample 1 60.1 ng/uL, sample 2 31.5 ng/uL | ||
| + | |||
| + | During the miniprep the filtered LB medium was poured back into the culture (so cells could grow again). | ||
| + | A -80 C stock was made with 1 mL culture (sample 1). | ||
Revision as of 09:21, 18 July 2013
Sander
prepared 20 tubes for use in -80 c Glyserol stock
Inne Cells with BBa_k818000, inoculated overnight, were successfully grown. Both negative controls were clear, and the rest of the samples was turbid. Did a miniprep of the samples 1 & 2 (the ones with Amp). Afterwards concentration of DNA was determined via nanodrop.
Results: sample 1 60.1 ng/uL, sample 2 31.5 ng/uL
During the miniprep the filtered LB medium was poured back into the culture (so cells could grow again). A -80 C stock was made with 1 mL culture (sample 1).
