Team:Groningen/Labwork/18 July 2013

From 2013.igem.org

(Difference between revisions)
Line 8: Line 8:
<br>Afterwards concentration of DNA was determined via nanodrop.
<br>Afterwards concentration of DNA was determined via nanodrop.
-
<br>Results: sample 1 60.1 ng/uL, sample 2 31.5 ng/uL
+
<br>Results: sample 1 = 60.1 ng/uL, sample 2 = 31.8 ng/uL
<br>During the miniprep the filtered LB medium was poured back into the culture (so cells could grow again).
<br>During the miniprep the filtered LB medium was poured back into the culture (so cells could grow again).
<br>A -80 C stock was made with 1 mL culture (sample 1).
<br>A -80 C stock was made with 1 mL culture (sample 1).

Revision as of 09:24, 18 July 2013

Sander
prepared 20 tubes for use in -80 c Glyserol stock

Inne & Mirjam
Cells with BBa_k818000, inoculated overnight, were successfully grown.
Both negative controls were clear, and the rest of the samples was turbid.
Did a miniprep of the samples 1 & 2 (the ones with Amp).
Afterwards concentration of DNA was determined via nanodrop.


Results: sample 1 = 60.1 ng/uL, sample 2 = 31.8 ng/uL


During the miniprep the filtered LB medium was poured back into the culture (so cells could grow again).
A -80 C stock was made with 1 mL culture (sample 1).