Team:Groningen/Labwork/18 July 2013

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Revision as of 15:10, 18 July 2013 by SanderWallert (Talk | contribs)

Sander
prepared 20 tubes for use in -80 c Glyserol stock.
created a restriction digest reaction for silk.


Inne & Mirjam
Cells with BBa_k818000, inoculated overnight, were successfully grown.
Both negative controls were clear, and the rest of the samples was turbid.
Did a miniprep of the samples 1 & 2 (the ones with Amp).

# composition Bacteria
1 4mL LB 8uL Amp +
2 4mL LB 8uL Amp +
3 4mL LB 8uL Amp (negative control) -
4 4mL LB +
5 4mL LB (negative control) -


Afterwards concentration of DNA was determined via nanodrop.


Results: sample 1 = 60.1 ng/uL, sample 2 = 31.8 ng/uL


During the miniprep the filtered LB medium was poured back into the culture (so cells could grow again).
A -80 C stock was made with 1 mL culture (sample 1). (stored in tray: iGEM 2012 A, compartment 70)