Team:Groningen/Labwork/1 August 2013

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<h2>Mirjam</h2>
<h2>Mirjam</h2>
Plates for the transformation of &Delta;Des, &Delta;CheY and &Delta;Des/&Delta;CheY did not show any colonies. The plates for eYFP showed some colonies on the plates with the transformation to <i>E.coli</i>. The plates with RFP and the ligation of Pdes/Chey did not show any colonies. Also the plates with a transformation of GFP240 and GFP0840 showed colonies.
Plates for the transformation of &Delta;Des, &Delta;CheY and &Delta;Des/&Delta;CheY did not show any colonies. The plates for eYFP showed some colonies on the plates with the transformation to <i>E.coli</i>. The plates with RFP and the ligation of Pdes/Chey did not show any colonies. Also the plates with a transformation of GFP240 and GFP0840 showed colonies.
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<br>Inoculated the colonies into LB with ampicillin to perform a miniprep later on
+
<br>Inoculated the colonies into LB with ampicillin to perform a miniprep later on.
-
<br>Made a new restriction digestion for RFP, eYFP and BBa_k823823 + LacI with EcoRI and PstI.
+
<br>Made a new restriction digestion for RFP, eYFP and BBa_k823823 + LacI with EcoRI and PstI. Did a gel purification to obtain the purified fragment.
 +
<br>Inoculated <i>B.subtilis</i>cells for transformation.
 +
 
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Revision as of 07:36, 1 August 2013

Mirjam

Plates for the transformation of ΔDes, ΔCheY and ΔDes/ΔCheY did not show any colonies. The plates for eYFP showed some colonies on the plates with the transformation to E.coli. The plates with RFP and the ligation of Pdes/Chey did not show any colonies. Also the plates with a transformation of GFP240 and GFP0840 showed colonies.
Inoculated the colonies into LB with ampicillin to perform a miniprep later on.
Made a new restriction digestion for RFP, eYFP and BBa_k823823 + LacI with EcoRI and PstI. Did a gel purification to obtain the purified fragment.
Inoculated B.subtiliscells for transformation.