Team:Groningen/Labwork/20 August 2013

From 2013.igem.org

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<br>
<br>
<br>The synthetic DNA sequences are prepared for use. They are diluted in 20&micro;l TE buffer.
<br>The synthetic DNA sequences are prepared for use. They are diluted in 20&micro;l TE buffer.
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 +
<h2>Inne</h2>
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The 0.7% agar plates that were to dry in the 37C incubator have dried. They were inoculated with liquid culture or from colonies on plates. The resulting in the following plates:
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<table border="1">
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<tr>
 +
<td>#</td>
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<td>strain</td>
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<td>inoculated with</td>
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<tr/>
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<tr>
 +
<td>1</td>
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<td>wt</td>
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<td>tooth pick from colony</td>
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<tr/>
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<td>2</td>
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<td>wt</td>
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<td>tooth pick from colony</td>
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<tr/>
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<td>3</td>
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<td>Delta cheY</td>
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<td>tooth pick from colony</td>
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<tr/>
 +
<td>4</td>
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<td>Delta cheY</td>
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<td>tooth pick from colony</td>
 +
<tr/>
 +
<td>5</td>
 +
<td>wt</td>
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<td>2,5 uL overnight culture</td>
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<tr/>
 +
<td>6</td>
 +
<td>wt</td>
 +
<td>2,5 uL overnight culture</td>
 +
<tr/>
 +
<td>7</td>
 +
<td>Delta cheY</td>
 +
<td>2,5 uL overnight culture</td>
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<tr/>
 +
<td>8</td>
 +
<td>Delta cheY</td>
 +
<td>2,5 uL overnight culture</td>
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<tr/>
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<td>9</td>
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<td>Negative control</td>
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<td>empty toothpick</td>
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<tr/>
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</table>
</div>
</div>

Revision as of 21:20, 20 August 2013

Claudio

BBa_J04450 is transformed to E. Coli DH5α on LB + Cm.
This BioBrick has to aims: the part codes for an RFP, which is necessary for submitting new backbone to the registry and the backbone which must used to submit new part to the registry.

The synthetic DNA sequences are prepared for use. They are diluted in 20µl TE buffer.

Inne

The 0.7% agar plates that were to dry in the 37C incubator have dried. They were inoculated with liquid culture or from colonies on plates. The resulting in the following plates:
# strain inoculated with
1 wt tooth pick from colony
2 wt tooth pick from colony
3 Delta cheY tooth pick from colony
4 Delta cheY tooth pick from colony
5 wt 2,5 uL overnight culture
6 wt 2,5 uL overnight culture
7 Delta cheY 2,5 uL overnight culture
8 Delta cheY 2,5 uL overnight culture
9 Negative control empty toothpick