Team:Groningen/Labwork/20 August 2013

From 2013.igem.org

(Difference between revisions)
Line 40: Line 40:
<td>1</td>
<td>1</td>
<td>wt</td>
<td>wt</td>
-
<td>tooth pick from colony</td>
+
<td>toothpick from colony</td>
<tr/>
<tr/>
<td>2</td>
<td>2</td>
<td>wt</td>
<td>wt</td>
-
<td>tooth pick from colony</td>
+
<td>toothpick from colony</td>
<tr/>
<tr/>
<td>3</td>
<td>3</td>
<td>Delta cheY</td>
<td>Delta cheY</td>
-
<td>tooth pick from colony</td>
+
<td>toothpick from colony</td>
<tr/>
<tr/>
<td>4</td>
<td>4</td>
<td>Delta cheY</td>
<td>Delta cheY</td>
-
<td>tooth pick from colony</td>
+
<td>toothpick from colony</td>
<tr/>
<tr/>
<td>5</td>
<td>5</td>

Revision as of 21:21, 20 August 2013

Claudio

BBa_J04450 is transformed to E. Coli DH5α on LB + Cm.
This BioBrick has to aims: the part codes for an RFP, which is necessary for submitting new backbone to the registry and the backbone which must used to submit new part to the registry.

The synthetic DNA sequences are prepared for use. They are diluted in 20µl TE buffer.

Inne

The 0.7% agar plates that were to dry in the 37C incubator have dried. They were inoculated with liquid culture or from colonies on plates. The resulting in the following plates:
# strain inoculated with
1 wt toothpick from colony
2 wt toothpick from colony
3 Delta cheY toothpick from colony
4 Delta cheY toothpick from colony
5 wt 2,5 uL overnight culture
6 wt 2,5 uL overnight culture
7 Delta cheY 2,5 uL overnight culture
8 Delta cheY 2,5 uL overnight culture
9 Negative control empty toothpick