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Team:Groningen/Labwork/22 July 2013
From 2013.igem.org
(Difference between revisions)
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Made a new restriction digest for silk without strep tag. | Made a new restriction digest for silk without strep tag. | ||
| + | |||
| + | '''Inne''' | ||
| + | <br> ran Gel to check everything that was in the freezer before trowing it away. | ||
| + | <br> Gel was 1.5% and ran at 90V for 45 min | ||
| + | |||
| + | <br>Checked tubes: | ||
| + | <table border = "1"> | ||
| + | <tr> | ||
| + | <td>Slot</td> | ||
| + | <td>Description Lid</td> | ||
| + | <td>Description Side</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>1</td> | ||
| + | <td>1kb generuler</td> | ||
| + | <td>Fermentas 1kb DNA Ladder (and more)</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>2</td> | ||
| + | <td>1</td> | ||
| + | <td>Silk 4.1ng/uL Strep R-F</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>3</td> | ||
| + | <td>1</td> | ||
| + | <td>Silk 4.1ng/uL F-R</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>4</td> | ||
| + | <td>2</td> | ||
| + | <td>Silk 4.6ng/uL Strep R-F</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>5</td> | ||
| + | <td>2</td> | ||
| + | <td>Silk 4.3ng/uL Strep R-F</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>6</td> | ||
| + | <td>3</td> | ||
| + | <td>Silk 4.9ng/uL R-strepF</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>7</td> | ||
| + | <td>3</td> | ||
| + | <td>Silk 5.9 ng/uL R-strepF</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>8</td> | ||
| + | <td>Silk without strep tag</td> | ||
| + | <td>10-07-13 Silk without strep tag 5.1 ng/uL</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>9</td> | ||
| + | <td>2</td> | ||
| + | <td>16-07 Silk</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>10</td> | ||
| + | <td>3</td> | ||
| + | <td>16-07 Silk</td> | ||
| + | </tr> | ||
| + | </table> | ||
Revision as of 09:21, 22 July 2013
Mirjam
Made a PCR for silk 2 and 3 (strepF-R and F-strepR)in triplo
- 28 ul MQ
- 1.5 ul DMSO
- 10 ul GC buffer
- 1 ul of each dNTP
- 2.5 ul primer F
- 2.5 ul primer R
- 0.5 ul phusion
- 1 ul genomic DNA (BBa_...016)
Run a gel with the ligation product of silk and the PCR afterwards. Figured out why the ligation did not work.
Made a new restriction digest for silk without strep tag.
Inne
ran Gel to check everything that was in the freezer before trowing it away.
Gel was 1.5% and ran at 90V for 45 min
Checked tubes:
| Slot | Description Lid | Description Side |
| 1 | 1kb generuler | Fermentas 1kb DNA Ladder (and more) |
| 2 | 1 | Silk 4.1ng/uL Strep R-F |
| 3 | 1 | Silk 4.1ng/uL F-R |
| 4 | 2 | Silk 4.6ng/uL Strep R-F |
| 5 | 2 | Silk 4.3ng/uL Strep R-F |
| 6 | 3 | Silk 4.9ng/uL R-strepF |
| 7 | 3 | Silk 5.9 ng/uL R-strepF |
| 8 | Silk without strep tag | 10-07-13 Silk without strep tag 5.1 ng/uL |
| 9 | 2 | 16-07 Silk |
| 10 | 3 | 16-07 Silk |
