Team:Groningen/Labwork/23 August 2013

From 2013.igem.org

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<h2>Sander</h2>
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made a plasmid isolation of S1, S2 S5 and S6. placed in a box marked bio bricks in locations 1,2,3 and 4 respectively. also placed the submission backbone in to that box on the lowest row.
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Revision as of 11:57, 23 August 2013

Claudio

S15, S16 and BBa_K823023 were digested with EcoRI and BamHI.
S3, S4, S9, S10 and BBa_K823023 were digested with BamHI and PstI.

Sebas

The 6 inoculated hy_spank-GFPdsm colonies grew overnight, preformed a miniprep (conc: >100ng/µl). Did a digestion check
with XbaI*PstI, expected sizes were: 759, 7544.
All plasmid purification's had the same sizes around ~750 and 7000~8000:

Sander

made a plasmid isolation of S1, S2 S5 and S6. placed in a box marked bio bricks in locations 1,2,3 and 4 respectively. also placed the submission backbone in to that box on the lowest row.