Team:Groningen/Labwork/23 July 2013

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Revision as of 09:16, 23 July 2013 by Inne (Talk | contribs)

Mirjam
Run a gel with the PCR products of silk without strep + signal sequence.
The PCR of silk without strep + signal sequence is successful. A new 1.5% agarose gel is made to do gel purification. Did the gel purification.

Run a gel with the purified PCR product of silk strepF-R and silk F-strepR.
The products are present in 2/3 samples for each silk type. These are combined and concentrated. Concentrations of 18.1 and 16.1 ng/ul are obtained. So a restriction digestion can be made.

Made a restriction digestion for the signal sequences.


Sander
Made a restriction digestion for silk strepF-R and silk F-strepR.

Inne
E. coli with the BBa_818000 backbone that had to grow overnight grew.

Sample Composition BBa_k818000 from growth
1 4 mL LB, 8 uL Amp iGEM 2012 Yes
2 4 mL LB, 8 uL Amp iGEM 2012 Yes
3 4 mL, LB 8 uL Amp (negative control) iGEM 2012 No
4 4 mL LB, 8 uL Amp, 4 uL IPTG iGEM 2012 Yes
5 4 mL LB, 8 uL Amp, 4 uL IPTG iGEM 2012 Yes
6 4 mL, 8uL Amp iGEM 2013 Yes
7 4 mL, 8 uL Amp, 8uL IPTG iGEM 2013 Yes
8 4 mL, 8 uL Amp (negative control) iGEM 2013 No

All suspensions were white. Decided to do a miniprep + digestion to see is plasmid was present. Samples 1 and 6 were chosen, 1 to check if renewed 2012 inoculation was a success and 6 to check if our stock had a the backbone. Upon down spinning the cells (first step of miniprep) a pinkish color was detected in the pellet of cell sample 6. This suggest that the BBa_k818000 backbone was in there after all. Sample 1 showed a less conclusive result. The cells weren't pure white, but also didn't display the same level of pinkness as sample 6.