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Team:Groningen/Labwork/24 July 2013
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(Created page with "'''Mirjam''' <br/>Run the samples of the PCR made 23-07 over a 1.5% agarose gel. Made a restriction digestion of the rest of the samples of silk 2 and 3 (strepF-R and F-strepR) ...") |
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Made a restriction digestion of the rest of the samples of silk 2 and 3 (strepF-R and F-strepR) to obtain a higher concentration. | Made a restriction digestion of the rest of the samples of silk 2 and 3 (strepF-R and F-strepR) to obtain a higher concentration. | ||
| + | |||
| + | '''Inne''' | ||
| + | <br>Made a -80 stock of the Munich BBa_k823023 backbone + our IPTG promoter. | ||
| + | |||
| + | <br>Made a miniprep of the inoculated cells with the k077ss7 biobrick that Chicago requested. | ||
| + | <br>Concentration of the final sample is 21.1 ng/uL. | ||
Revision as of 08:59, 24 July 2013
Mirjam
Run the samples of the PCR made 23-07 over a 1.5% agarose gel.
Made a restriction digestion of the rest of the samples of silk 2 and 3 (strepF-R and F-strepR) to obtain a higher concentration.
Inne
Made a -80 stock of the Munich BBa_k823023 backbone + our IPTG promoter.
Made a miniprep of the inoculated cells with the k077ss7 biobrick that Chicago requested.
Concentration of the final sample is 21.1 ng/uL.
