Team:Groningen/Labwork/24 July 2013

From 2013.igem.org

Mirjam
Run the samples of the PCR made 23-07 over a 1.5% agarose gel. The samples show the presence of the expected bands for 3 out of 4 signal sequences. So it is tried to obtain these samples by Gel extraction.

Made a restriction digestion of the rest of the samples of silk 2 and 3 (strepF-R and F-strepR) to obtain a higher concentration.

The primers that are ordered for heat motility are found and diluted. The first PCR is made to obtain Pdes.

Inne
Made a -80 stock of the Munich BBa_k823023 backbone + our IPTG promoter.

Made a miniprep of the inoculated cells with the k077557 biobrick that Chicago requested.
Concentration of the final sample is 21.1 ng/uL.
Sample was stored in -20 freezer.