Team:Groningen/Labwork/26 July 2013

Mirjam

Made a gel to check the gel purified products of Pdes, CheY and des DOWN. These products look fine. A check of the PCR products of CheY UP, spec and CheY DOWN revealed that the PCR for spec failed again. So a new PCR reaction for spec is made.

Gel purification is done for CheY UP, CheY DOWN and Tet.

New PCR reactions are made for all types of silk we want to create. This time the bp time is increased to 30 sec, to examine if this helps to obtain a higher concentration.

Did a restriction digestion with BamHI for Pdes and CheY. The restriction digestion for Pdes failed. So a new restriction digestion is made.

Did a restriction digestion for RFP and eYFP obtained from the biobrick plates.

Claudio

The Bacillus Subtilis plates incubated overnight show colonies.
One colony is picked and transformed with the plasmid LacI promoter in backbone BBa_k823823.
The transformants are plated on:

• LB agar + chloramphenicol
• LB agar + chloramphenicol + IPTG