Team:Groningen/Labwork/26 September 2013

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<h2>Mirjam</h2>
 
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<h2>Mirjam</h2>
<h2>Mirjam</h2>
Checked the plates with MotB+S9+S5 and FliZ+S3+S5. These plates showed only red colonies.
Checked the plates with MotB+S9+S5 and FliZ+S3+S5. These plates showed only red colonies.
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<br>Made a ligation between EstA and the S3 and S9 subunit.  
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<br>Made a restriction digestion of EstA, S3 and S9 with BamHI. Made a ligation between EstA and the S3 and S9 subunit.
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<h2>Claudio & Sander</h2>
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First we now realize that iodine kills <i>Bacillus subtilis</i>.
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<br>6 colonies from each <i>Bacillus subtilis</i> transformation plate (BBa_K1085014-BBa_K1085018 and BBa_K1085014-BBa_K1085022) were inoculated in LB + Cm and were streaked out on LB agar + Cm + starch.
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<br>
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<h2>Inne</h2>
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Ran a 0.8% agarose gel to check the results from the colony PCR, 12 samples were tested. mixture 5uL sample + 5uL 2x loading dye. gel ran at 90 Volts for 35 minutes.
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<br>
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Gel composition:
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<br>Gel 2
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<table border = "1">
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<td>Ladder</td>
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<td>1</td>
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<td>2</td>
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<td>3</td>
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<td>4</td>
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<td>5</td>
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<td>6</td>
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</tr>
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<tr>
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<td rowspan = "3"><br><br><br> </td>
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<td rowspan = "3"><br><br><br> </td>
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<td rowspan = "3"><br><br><br> </td>
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<td rowspan = "3"><br><br><br> </td>
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<td rowspan = "3"><br><br><br> </td>
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<td rowspan = "3"><br><br><br> </td>
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<td rowspan = "3"><br><br><br> </td>
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</tr>
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<tr>
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</tr>
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<tr>
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</tr>
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<tr>
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<td>Ladder</td>
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<td>7</td>
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<td>8</td>
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<td>9</td>
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<td>10</td>
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<td>11</td>
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<td>12</td>
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</tr>
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Latest revision as of 17:09, 26 September 2013

Mirjam

Checked the plates with MotB+S9+S5 and FliZ+S3+S5. These plates showed only red colonies.
Made a restriction digestion of EstA, S3 and S9 with BamHI. Made a ligation between EstA and the S3 and S9 subunit.

Claudio & Sander

First we now realize that iodine kills Bacillus subtilis.
6 colonies from each Bacillus subtilis transformation plate (BBa_K1085014-BBa_K1085018 and BBa_K1085014-BBa_K1085022) were inoculated in LB + Cm and were streaked out on LB agar + Cm + starch.

Inne

Ran a 0.8% agarose gel to check the results from the colony PCR, 12 samples were tested. mixture 5uL sample + 5uL 2x loading dye. gel ran at 90 Volts for 35 minutes.
Gel composition:
Gel 2
Ladder 1 2 3 4 5 6





















Ladder 7 8 9 10 11 12