Team:Groningen/Labwork/26 September 2013

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Revision as of 17:07, 26 September 2013 by Inne (Talk | contribs)

Mirjam

Checked the plates with MotB+S9+S5 and FliZ+S3+S5. These plates showed only red colonies.
Made a restriction digestion of EstA, S3 and S9 with BamHI. Made a ligation between EstA and the S3 and S9 subunit.

Claudio & Sander

First we now realize that iodine kills Bacillus subtilis.
6 colonies from each Bacillus subtilis transformation plate (BBa_K1085014-BBa_K1085018 and BBa_K1085014-BBa_K1085022) were inoculated in LB + Cm and were streaked out on LB agar + Cm + starch.

Inne

Ran a 0.8% agarose gel to check the results from the colony PCR, 12 samples were tested. mixture 5uL sample + 5uL 2x loading dye. gel ran at 90 Volts for 35 minutes.
Gel composition:
Gel 2
Ladder 1 2 3 4 5 6 empty empty/td> empty empty

































Ladder 7 8 9 10 11 12 empty empty empty empty