Team:Groningen/Labwork/27 July 2013

From 2013.igem.org

(Difference between revisions)
Line 26: Line 26:
<br>Only the plates transformed with the modified Munich backbone show colonies which are all white at a glance. None of the colonies grown on LB with IPTG are red.
<br>Only the plates transformed with the modified Munich backbone show colonies which are all white at a glance. None of the colonies grown on LB with IPTG are red.
<br>
<br>
-
<br>Colony PCR is performed on 8 colonies using an annealing temperature of 55&deg;C in order to double check the integration of the modified Munich backbone plasmid in the Bacillus Subtilis genome.
+
<br>Colony PCR is performed on 8 colonies using an annealing temperature of 46&deg;C in order to double check the integration of the modified Munich backbone plasmid in the Bacillus Subtilis genome.
</div>
</div>

Revision as of 13:51, 29 July 2013

Claudio


Only the plates transformed with the modified Munich backbone show colonies which are all white at a glance. None of the colonies grown on LB with IPTG are red.

Colony PCR is performed on 8 colonies using an annealing temperature of 46°C in order to double check the integration of the modified Munich backbone plasmid in the Bacillus Subtilis genome.