Team:Groningen/Labwork/27 July 2013
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<br>Only the plates transformed with the modified Munich backbone show colonies which are all white at a glance. None of the colonies grown on LB with IPTG are red. | <br>Only the plates transformed with the modified Munich backbone show colonies which are all white at a glance. None of the colonies grown on LB with IPTG are red. | ||
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- | <br>Colony PCR is performed on 8 colonies using an annealing temperature of | + | <br>Colony PCR is performed on 8 colonies using an annealing temperature of 46°C in order to double check the integration of the modified Munich backbone plasmid in the Bacillus Subtilis genome. |
</div> | </div> |
Revision as of 13:51, 29 July 2013
Claudio
Only the plates transformed with the modified Munich backbone show colonies which are all white at a glance. None of the colonies grown on LB with IPTG are red.
Colony PCR is performed on 8 colonies using an annealing temperature of 46°C in order to double check the integration of the modified Munich backbone plasmid in the Bacillus Subtilis genome.