Team:Groningen/Labwork/30 July 2013

From 2013.igem.org

(Difference between revisions)
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<h2>Mirjam</h2>
<h2>Mirjam</h2>
Started gel extraction for the restricted backbone+promoter, RFP and eYFP. RFP and eYFP are dissolved in 30&micro;l elution buffer, to get a higher concentration
Started gel extraction for the restricted backbone+promoter, RFP and eYFP. RFP and eYFP are dissolved in 30&micro;l elution buffer, to get a higher concentration
-
<br>Run a 0.8% agarose gel to examine the restriction.
+
<br>Run a 0.8% agarose gel to examine the restriction. Gel revealed only a very small band for RFP.
<br>Did a nanodrop on the samples to examine their concentration. Concentration of the backbone is 0.8 ng/&micro;l, RFP 6.4 ng/&micro;l and eYFP 2.4 ng/&micro;l.
<br>Did a nanodrop on the samples to examine their concentration. Concentration of the backbone is 0.8 ng/&micro;l, RFP 6.4 ng/&micro;l and eYFP 2.4 ng/&micro;l.

Revision as of 07:40, 30 July 2013

Mirjam

Started gel extraction for the restricted backbone+promoter, RFP and eYFP. RFP and eYFP are dissolved in 30µl elution buffer, to get a higher concentration
Run a 0.8% agarose gel to examine the restriction. Gel revealed only a very small band for RFP.
Did a nanodrop on the samples to examine their concentration. Concentration of the backbone is 0.8 ng/µl, RFP 6.4 ng/µl and eYFP 2.4 ng/µl.