Team:Groningen/Labwork/30 July 2013

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<br>Run a 0.8% agarose gel to examine the restriction. Gel revealed only a very small band for RFP.
<br>Run a 0.8% agarose gel to examine the restriction. Gel revealed only a very small band for RFP.
<br>Did a nanodrop on the samples to examine their concentration. Concentration of the backbone is 0.8 ng/&micro;l, RFP 6.4 ng/&micro;l and eYFP 2.4 ng/&micro;l.
<br>Did a nanodrop on the samples to examine their concentration. Concentration of the backbone is 0.8 ng/&micro;l, RFP 6.4 ng/&micro;l and eYFP 2.4 ng/&micro;l.
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<br>Did a new restriction digestion for BBa_k823823 + LacI with EcoRI and PstI, using 500 and 250ng.
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<h2>Chaline & Mirjam</h2>
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Did a restriction digestion for eYFP with EcoRI and PstI using 200ng.
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Revision as of 07:55, 30 July 2013

Mirjam

Started gel extraction for the restricted backbone+promoter, RFP and eYFP. RFP and eYFP are dissolved in 30µl elution buffer, to get a higher concentration
Run a 0.8% agarose gel to examine the restriction. Gel revealed only a very small band for RFP.
Did a nanodrop on the samples to examine their concentration. Concentration of the backbone is 0.8 ng/µl, RFP 6.4 ng/µl and eYFP 2.4 ng/µl.
Did a new restriction digestion for BBa_k823823 + LacI with EcoRI and PstI, using 500 and 250ng.

Chaline & Mirjam

Did a restriction digestion for eYFP with EcoRI and PstI using 200ng.