Team:Groningen/Labwork/3 August 2013

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On the 2x SG plate, which was dried over night at 37&deg;C, 2&micro;l of overnight <i>Bacillus Subtilis</i> culture is spotted.
On the 2x SG plate, which was dried over night at 37&deg;C, 2&micro;l of overnight <i>Bacillus Subtilis</i> culture is spotted.
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<br>The plates (plated yesterday) show colonies. A few colonies are picked and streaked out on LB + Amp + IPTG (1mM).
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<br>The plates (plated yesterday) show colonies. A few colonies are picked and streaked out on LB + Amp + IPTG (100&micro;M).
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<br>
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<br>The plasmid (isolated by <b>Sander</b>) that seems to contain the desired construct is transformed in <i>E. Coli</i> DH5&alpha; and plated on LB + Amp + IPTG (1mM).
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<br>The plasmid (isolated by <b>Sander</b>) that seems to contain the desired construct is transformed in <i>E. Coli</i> DH5&alpha; and plated on LB + Amp + IPTG (100&micro;M).
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Revision as of 13:38, 3 August 2013

Claudio

On the 2x SG plate, which was dried over night at 37°C, 2µl of overnight Bacillus Subtilis culture is spotted.

The plates (plated yesterday) show colonies. A few colonies are picked and streaked out on LB + Amp + IPTG (100µM).

The plasmid (isolated by Sander) that seems to contain the desired construct is transformed in E. Coli DH5α and plated on LB + Amp + IPTG (100µM).