Team:Groningen/Labwork/3 August 2013
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On the 2x SG plate, which was dried over night at 37°C, 2µl of overnight <i>Bacillus Subtilis</i> culture is spotted. | On the 2x SG plate, which was dried over night at 37°C, 2µl of overnight <i>Bacillus Subtilis</i> culture is spotted. | ||
<br> | <br> | ||
- | <br>The plates (plated yesterday) show colonies. A few colonies are picked and streaked out on LB + Amp + IPTG ( | + | <br>The plates (plated yesterday) show colonies. A few colonies are picked and streaked out on LB + Amp + IPTG (100µM). |
<br> | <br> | ||
- | <br>The plasmid (isolated by <b>Sander</b>) that seems to contain the desired construct is transformed in <i>E. Coli</i> DH5α and plated on LB + Amp + IPTG ( | + | <br>The plasmid (isolated by <b>Sander</b>) that seems to contain the desired construct is transformed in <i>E. Coli</i> DH5α and plated on LB + Amp + IPTG (100µM). |
</div> | </div> |
Revision as of 13:38, 3 August 2013
Claudio
On the 2x SG plate, which was dried over night at 37°C, 2µl of overnight Bacillus Subtilis culture is spotted.The plates (plated yesterday) show colonies. A few colonies are picked and streaked out on LB + Amp + IPTG (100µM).
The plasmid (isolated by Sander) that seems to contain the desired construct is transformed in E. Coli DH5α and plated on LB + Amp + IPTG (100µM).