Team:Groningen/Labwork/3 September 2013
From 2013.igem.org
(Difference between revisions)
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<br>pSB1C3-S1 and pSB1C3-S2 were digested with Spei and PstI. | <br>pSB1C3-S1 and pSB1C3-S2 were digested with Spei and PstI. | ||
<br>The samples were checked using gel electrophoresis (agarose 0.8%). | <br>The samples were checked using gel electrophoresis (agarose 0.8%). | ||
+ | |||
+ | <h2>Sander</h2> | ||
+ | made an inoculation of s1,2,5,6,7,8,11,12,13 an 14. | ||
</div> | </div> |
Revision as of 09:30, 4 September 2013
Claudio
PCR using as template S5 and primer S16-F along S16-R was performed. This new pair of primers was tested at two diffrent annealing temperature 60°C and 62°C, respectively.The presence of the expected products was checked using gel electrophoresis (agarose 0.8%).
The PCR worked and the samples were purified.
The PCR product (S5) was digested with XbaI and PstI and purified.
pSB1C3-S1 and pSB1C3-S2 were digested with Spei and PstI.
The samples were checked using gel electrophoresis (agarose 0.8%).