Team:Groningen/Labwork/3 September 2013

Claudio

PCR using as template S5 and primer S16-F along S16-R was performed. This new pair of primers was tested at two diffrent annealing temperature 60°C and 62°C, respectively.
The presence of the expected products was checked using gel electrophoresis (agarose 0.8%).
The PCR worked and the samples were purified.
The PCR product (S5) was digested with XbaI and PstI and purified.

pSB1C3-S1 and pSB1C3-S2 were digested with Spei and PstI.
The samples were checked using gel electrophoresis (agarose 0.8%).

Sander

made an inoculation of s1,2,5,6,7,8,11,12,13 an 14.